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Method for preparing and purifying grifola frondosa polysaccharide with high antioxidant activity

A technology of Grifola frondosa polysaccharide and oxidation activity, applied in biochemical equipment and methods, microorganism-based methods, and adding compounds to stimulate growth, etc., can solve the problems of unsatisfactory application in the market, limited production of acidic polysaccharide components, etc. , to achieve the effect of improving in vitro antioxidant activity, safety and non-toxic stability, and improving reducing ability

Active Publication Date: 2021-08-06
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The efficacy of Grifola frondosa has been widely recognized, but the production of acidic polysaccharide components of Grifola frondosa limits the application of the market and cannot meet the growing demand of people

Method used

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  • Method for preparing and purifying grifola frondosa polysaccharide with high antioxidant activity
  • Method for preparing and purifying grifola frondosa polysaccharide with high antioxidant activity
  • Method for preparing and purifying grifola frondosa polysaccharide with high antioxidant activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1 (control group)

[0065] Grifola frondosa seed culture: cut the Grifola frondosa grown on the PDA plate into 1cm 2 Take 3 to 5 small pieces of different sizes and inoculate them into the seed medium in a 100mL / 250mL Erlenmeyer flask, culture at 160r / min and 28°C for 5 days.

[0066] Fermentation culture of Grifola frondosa: add 150mL fermentation medium to a 500mL Erlenmeyer flask, inoculate 15mL of seed liquid, culture at 160r / min, 28°C for 8 days.

[0067] The seed medium contains 200 g of potatoes, 20 g of glucose, 5 g of peptone, 2 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate heptahydrate, and vitamin B per L. 1 0.02g, pH natural. The fermentation medium contains 22g of glucose per L, 3g of peptone, 1.2g of potassium dihydrogen phosphate, 0.8g of magnesium sulfate heptahydrate, vitamin B 1 0.12g, pH natural.

[0068] Wherein the extraction method and assay method of Grifola frondosa polysaccharide are:

[0069] Take 1 mL of the abo...

Embodiment 2

[0071]The culture medium and culture method are the same as in Example 1, the difference is that on the 0-2d of the fermentation culture of Grifola frondosa, 0-0.2mmol / L of exogenous additive farnesol is added, and the fermentation liquid of Grifola frondosa after adding farnesol continues to ferment to 8d. After the fermentation is finished, the mycelia of Grifola frondosa is obtained and the polysaccharides in the fermentation broth are extracted. Wherein, the extraction method and assay method of the polysaccharide of Grifola frondosa fermented liquid are the same as Example 1, and the polysaccharide content of measured Grifola frondosa fermented liquid is (0.8371 ± 0.0532) g / L, which has increased by 66% relative to the control group. Such as figure 2 shown.

Embodiment 3

[0073] The culture medium and culture method are the same as in Example 1, the difference is that on the 0-2d of the fermentation culture of Grifola frondosa, 0.2-0.4mmol / L of exogenous additive farnesol is added, and the fermentation liquid of Grifola frondosa after adding farnesol continues to ferment to 8d. After the fermentation is finished, the mycelia of Grifola frondosa is obtained and the polysaccharides in the fermentation broth are extracted. Wherein, the extraction method and assay method of the polysaccharide of Grifola frondosa fermented liquid are the same as Example 1, and the polysaccharide content of measured Grifola frondosa fermented liquid is (1.0058 ± 0.0128) g / L, which has increased by 98% relative to the control group. Such as image 3 shown.

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Abstract

The invention discloses a method for preparing and purifying grifola frondosa polysaccharide with high in-vitro antioxidant activity. According to the method, farnesol is added at beginning of or in a process of liquid fermentation of grifola frondosa. According to the method, farnesol is added in the liquid fermentation process of grifola frondosa mycelia, so that polysaccharide yield of grifola frondosa fermentation liquor is promoted to be changed, yield of acidic polysaccharide components generated by grifola frondosa fermentation is remarkably increased, the polysaccharide yield of the grifola frondosa fermentation liquor is remarkably increased on the basis of not increasing an original fermentation period, the maximum increase amplitude reaches 149%, content of the separated and purified acidic polysaccharide is increased from 7.4% to 58.2%, the in-vitro antioxidant activity of the separated and purified acidic polysaccharide is remarkably improved compared with that of a control group, and the exogenous additive farnesol is safe, non-toxic and high in stability, so that a foundation is laid for industrial production of polysaccharide in the grifola frondosa fermentation liquor.

Description

technical field [0001] The invention belongs to the technical field of Grifola frondosa polysaccharide extraction, in particular to a preparation and purification method of Grifola frondosa polysaccharide with high antioxidant activity. Background technique [0002] Grifola frondosa polysaccharide is the main bioactive component of the fermentation liquid of Grifola frondosa. A large number of pharmacological and pharmacological studies have proved that the polysaccharides of Grifola frondosa fermented liquid have good effects in anti-tumor, anti-HIV virus, and prevention and treatment of diabetes, hypertension, hyperlipidemia, obesity, and Alzheimer's disease, and some aspects even surpass the existing ones. Clinical application of lentinan, versicolor polysaccharide and schizophyllan. In addition, Grifola frondosa polysaccharide has many active functions beneficial to the human body. It can improve the body's ability to tolerate hypoxia, enhance the body's immunity, scav...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12N1/38C12N1/14C08B37/00C12R1/645
CPCC12P19/04C12N1/38C12N1/14C08B37/0003
Inventor 韩培培王晓丽陈旎李健张乐乐贾士儒钟成谭之磊
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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