A kind of skipjack antioxidant compound and preparation method thereof

An anti-oxidation and compound technology, applied in cosmetic preparations, anti-toxic agents, cosmetics and other directions, can solve the problems of rare research and application, large sample volume, high cost, etc., and achieves simple preparation process, easy industrialization, and easy preparation. The effect of absorption and digestion

Active Publication Date: 2020-07-03
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The amount of sample required for in vivo experiments is large, costly, and takes a long time, so few studies use it
However, the results of in vitro experiments can only be used for preliminary screening, and only those with antioxidant activity in vivo experiments have the possibility of further development and utilization.

Method used

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  • A kind of skipjack antioxidant compound and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of embodiment 1 bonito antioxidant complex

[0025] Take 2.5kg of bonito meat after removing viscera, gills, cleaning, cutting and mincing, add water according to the ratio of material to liquid (weight ratio) 1:4, mix well, adjust the pH to 7.5 with sodium hydroxide solution, and adjust the Pour the pH material into the enzymatic hydrolysis tank, turn on the power, start the heating and stirring switch, after the temperature rises to 50°C, add 25 IU of trypsin per gram of bonito meat, enzymolyze at 50°C for 4 hours, and then heat up Incubate at 90°C for 10 minutes to inactivate the enzyme. After the enzymolysis solution is cooled, it is centrifugally filtered by a three-legged centrifuge to remove impurities and insoluble matter, and an inorganic tubular ceramic membrane with a membrane pore size of 200nm is used to perform circulating microfiltration on the centrifuged enzymolysis solution. When the volume of the residual solution is about At 1...

Embodiment 2

[0026] Example 2 In vivo antioxidant experiment and results of skipjack antioxidant complex

[0027] SPF grade NIH mice, 30 males, with an initial body weight of 25-30 g. According to body weight, the animals were randomly divided into 3 groups (10 animals in each group), which were blank control group, model control group, and bonito compound group.

[0028] The dosage of skipjack fish compound was 0.5g / kg body weight, and 0.2mL / 10g body weight was administered by intragastric administration, and the model control group and the blank control group were intragastrically administered an equal volume of ultrapure water. Once a day for 30 consecutive days. After the last gavage, the model control group and skipjack compound group were fasted for 16 hours (overnight), and then 50% ethanol 12mL / kg BW was given by gavage once, and they were killed by cervical dislocation 6 hours later, and their livers were taken; the blank control group Without processing, can not help but eat th...

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Abstract

The invention provides a preparation method of a skipjack oxidation resistant compound. The pH value of skipjack fresh slurry is adjusted to 7.0 to 8.0, after enzymolysis is conducted, enzymatic hydrolysate is centrifuged and filtered twice, and then the skipjack oxidation resistant compound is obtained. According to the two times of filtration, a ceramic membrane is used for filtration at the first time, and a nanofiltration membrane is used for filtration at the second time. The peptide constituent below 1000 Daltons in the skipjack oxidation resistant compound accounts for over 95% and is easy to absorb and digest; the reducing power is equivalent to that of 0.125 mg / mL of glutathione when the concentration is 5 mg / mL; and the IC50 value of scavenging activity of a 1,1-diphenyl-picrylhydrazyl free radical is 2.68 mg / mL, the IC50 value of scavenging activity of a superoxide anion free radical is 16.14 mg / mL, and the IC50 value of scavenging activity of a hydroxyl free radical is 21.04 mg / mL. The preparation process is simple in technology, and industrialization is easy. The product passes in vitro experiments and in vivo experiments, it is shown that the product has the good oxidation resistance effect, and the good development and use prospects are shown.

Description

technical field [0001] The invention relates to the technical field of preparation of marine biological products, in particular to a skipjack antioxidant compound and a preparation method thereof. Background technique [0002] In the past two decades, the research on active oxygen and free radicals has become the frontier and hot spot of modern life science, and the evaluation and screening of natural resources with strong antioxidant activity has become a new trend in biology, medicine and food science research. Natural antioxidants in organisms mainly include reduced glutathione and carnosine, but the content is very low. Finding exogenous antioxidants has always been the focus of research, and the research on antioxidant peptides is the most popular. Marine and freshwater aquatic resources have become an important source of obtaining and preparing active peptides. On the one hand, the peptide chains of aquatic proteins generally contain functional regions, that is, peptid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A23J3/34A23J3/04A23J1/04A61K38/01A61P39/06A61K8/64A61Q19/08
CPCA23J1/04A23J3/04A23J3/341A61K8/64A61K38/00C12P21/06
Inventor 周春霞杨萍洪鹏志刘唤明
Owner GUANGDONG OCEAN UNIVERSITY
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