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This invention relates to a bacterium that degrades chlorobenzene pollutants and its applications. The bacterium is a *Pseudomonas putida* strain, named *Pseudomonas putida* BS-1, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 2024441 on March 11, 2024. Compared with existing technologies, the strain provided by this invention can completely degrade a complex pollutant mixture of dichlorobenzene, chlorobenzene, and benzene within 48 hours. It can survive for a long time and maintain its degradation activity under actual pollution conditions of complex pollution and low temperatures. Furthermore, the functional genes related to the degradation of chlorobenzene pollutants are located on a plasmid, which helps the indigenous bacterium acquire the relevant functions for degrading chlorobenzene pollutants. This strain can be applied to in-situ bioremediation of groundwater with complex chlorobenzene pollution in chemical industrial parks, effectively overcoming the technical shortcomings of traditional reduction dechlorination remediation technologies, which often result in incomplete reduction of products, and achieving green, sustainable, and thorough purification without secondary pollutants.

The invention relates to muscula seeking antioxidant peptide as well as a preparation method and application thereof, and belongs to the technical field of biology. The preparation method comprises the following steps: preparing enzymatic hydrolysate by using neutral protease as a raw material, carrying out ultrafiltration and gel chromatography separation and purification on the enzymatic hydrolysate, and then carrying out liquid chromatography-tandem mass spectrometry to obtain three antioxidant peptides, and the amino acid sequences of the three antioxidant peptides are KAGIALNDNFVKL, NQPGGQGNNPGQGAPN and LFSYSDTHRH. The preparation method is simple, and the prepared antioxidant peptide has a good scavenging effect on DPPH free radicals and ABTS free radicals. In cell antioxidant research, the content of malondialdehyde MDA in cells can be reduced to different degrees, the activity of SOD antioxidant enzyme can be improved, and the content of cytokines in oxidative damage cells can be effectively inhibited.

The invention discloses a sulfoxide-substituted p-menthane compound as well as a preparation method and application thereof. The compound has a structure as shown in a formula I, and can be prepared by hydrosulfide and oxidation reaction of 1-methyl-4-(1-methyl ethyl)-cyclohexene and mercaptans with different substituent groups. The method disclosed by the invention is simple and convenient to operate and mild in condition, and the prepared sulfoxide-substituted p-menthane compound has excellent herbicidal activity and can be used for preventing and treating agricultural weeds. # imgabs0 #