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Application and composition of organic solvent extract of ligia exotica

An organic solvent and extract technology, applied in the field of marine natural medicine, can solve the problem of unexplained analgesic and anti-inflammatory effects, and achieve the effects of significant analgesic and anti-inflammatory activities, no acute toxicity and high safety.

Pending Publication Date: 2020-09-29
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the material basis for the analgesic and anti-inflammatory effects of sea cockroaches has not yet been elucidated.

Method used

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  • Application and composition of organic solvent extract of ligia exotica
  • Application and composition of organic solvent extract of ligia exotica
  • Application and composition of organic solvent extract of ligia exotica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of ethanol extract of sea cockroaches: freshly caught sea cockroaches were weighed after removing impurities such as seaweed. At room temperature, according to the mass volume ratio (w / v, g / mL) 1:6.5, add ethanol with a volume concentration of 85% to the sea cockroach homogenate, soak and extract for 24 hours. After the first extraction, filter with 200-mesh sieve, concentrate the filtrate under reduced pressure at 45°C, remove the extraction solvent, and add the residue at a volume concentration of 85 Extract by soaking in % ethanol for 24 hours each time, and repeat the extraction twice. Then, the three filtrates were combined, and the combined filtrate was concentrated under reduced pressure with a rotary evaporator at 45° C. to finally obtain a black viscous extract, namely sea cockroach ethanol extract (hereinafter referred to as sea cockroach ethanol extract).

Embodiment 2

[0041] Take the sea cockroach alcohol extract extract in Example 1, add deionized water with a mass volume ratio of 1:2 (w / v, g / mL) and stir at room temperature. The above mixture was ultrasonically dispersed for 30 min at room temperature, so that the ethanol extract of sea cockroach was uniformly dispersed in the water phase. Then, the above mixture was transferred to a separatory funnel, and petroleum ether and ethyl acetate equal to the volume of the dispersion were added to the separatory funnel for extraction, and each solvent was extracted twice for 2 hours each time. Fully stand at room temperature for 6 hours after each extraction. Then, carefully transfer the petroleum ether extract and ethyl acetate extract respectively, combine the extracts under the same extraction solvent, concentrate under reduced pressure at 40°C to remove petroleum ether or ethyl acetate, and obtain the ethanol extract of sea cockroach Petroleum ether extract (PE), ethyl acetate extract (EE) ...

Embodiment 3

[0045] Take 50.0 g of dried sea cockroaches, pulverize them with a small pulverizer and pass through a 60-80 mesh sieve to obtain dry sea cockroach powders. Then, add petroleum ether, ethyl acetate, methanol, 95% ethanol by volume, 50% ethanol by volume, deionized The water was extracted three times each, and each extraction was 2h. After each extraction, the extracts under the same extraction solvent were combined, the extracts were filtered through a 200-mesh sieve, and the filtrate was centrifuged at 3000 rpm for 20 min at room temperature. Then, the extracts under the same extraction solvent were combined, and each extract was concentrated under reduced pressure using a rotary evaporator at 45 ° C to obtain petroleum ether extract (oily extract, 0.4 g), ethyl acetate extract ( Oily extract, 2.9g), methanol extract (solid powder, 11.1g), volume concentration 95% ethanol extract (solid powder, 0.3g), volume concentration 50% ethanol extract (solid powder, 7.7g), chamber Wa...

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Abstract

The invention relates to the field of marine natural medicines, in particular to application of an organic solvent extract of ligia exotica to preparation of analgesic and anti-inflammatory medicines,or analgesic and anti-inflammatory medicine compositions, or health-care products with analgesic and anti-inflammatory functions, or functional foods with analgesic and anti-inflammatory functions. Fresh bodies or dry bodies of ligia exotica are extracted by an organic solvent after being crushed, and then subjected to vacuum concentration to obtain an organic solvent extract of the ligia exotica. In-vitro tests show that the organic solvent extract of the ligia exotica has remarkable epoxidase (COX-2) inhibition activity. Cell tests and animal tests prove that the ligia exotica extract has astrong inhibition effect on nitric oxide (NO) secreted by macrophages RAW264.7, has a remarkable analgesic effect on experimental mice, and is free of acute toxicity and high in safety. Therefore, the organic solvent extract of the ligia exotica provided by the invention has the potential of being developed into the analgesic and anti-inflammatory medicines or medicine compositions, the health-care products and the functional foods.

Description

technical field [0001] The invention relates to the field of marine natural medicines, in particular to the use of sea cockroach organic solvent extracts in the preparation of analgesic and anti-inflammatory drugs, or analgesic and anti-inflammatory pharmaceutical compositions, or health products with analgesic and anti-inflammatory functions, or analgesic and anti-inflammatory drugs. Functional applications in functional foods. . Background technique [0002] According to the definition of the International Pain Society, pain is an unpleasant sensory and emotional experience associated with existing or potential tissue damage. Like breathing, pulse, blood pressure, and body temperature, pain is also listed as the "fifth vital sign." Pain is one of the major medical challenges facing mankind so far. Modern medicine believes that pain is not only a disease symptom, but pain itself is also a disease. Pain not only affects the quality of life of patients, but also brings hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/612A61P29/00A23L33/105
CPCA61K35/612A61P29/00A23L33/105A23V2002/00A23V2250/204
Inventor 岳洋张全斌王晶牛锡珍耿丽华
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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