Application of imperatorin in preparing medicament for preventing and treating hepatitis or liver injury
A technology for parsley and chemical liver injury, which is applied in the field of medicine and achieves the effects of high safety, low price and wide sources
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Embodiment 1
[0009] Example 1 Preparation of parsleydin B
[0010] Baizhi crude drug decoction pieces (Shanghai Yangpu Traditional Chinese Medicine Decoction Pieces Factory) are crushed and passed through a 120-mesh sieve. Take 1kg of powder and extract 2 times with 5L 95% ethanol under reflux for 2 hours each time. Combine the extracts, concentrate under reduced pressure, recover ethanol, and separate while hot Water layer. After the water layer is left to solidify, it is dissolved with 100ml of 40°C absolute ethanol and left to crystallize. The obtained crystals were recrystallized three times with 10 ml of 95% ethanol at 40°C, and purified with a macroporous adsorption resin to obtain 9.6 mg of parsleyin B. Detected by HPLC, the purity of parsleyin B was 93.2%.
Embodiment 2
[0011] Example 2 The effect of parsleyin B on the expression of hepatitis B virus HBsAg and HBeAg
[0012] 1. Experimental materials
[0013] DMEM medium: product of Gibco, USA, batch number 13200-035;
[0014] Calf serum: product of Gibco, USA, batch number 16000-036;
[0015] HBsAg detection kit: product of Beijing Kewei Clinical Diagnostic Reagent Biotechnology Co., Ltd., batch number: 200706003;
[0016] HBeAg detection kit: Beijing Kewei Clinical Diagnostic Reagent Biotechnology Co., Ltd. product, batch number: 200708008;
[0017] HepG 2.2.15 cells, provided by the Central Laboratory of Shanghai Changhai Hospital, are established by co-transfection of cloned diabody HBV DNA and anti-G418 resistance plasmid into human hepatocarcinoma cell line HepG2 cells, capable of transcribing and translating HBV Gene, and can secrete HBsAg and HBeAg stably into the culture supernatant for a long time. It is currently recognized and commonly used at home and abroad as a cell model for evaluating ...
Embodiment 3
[0034] Example 3 Protective effect of parsleyin B on immune liver injury
[0035] 1. Experimental materials
[0036] BCG lyophilized powder (BCG), purchased from the National Institute for the Control of Pharmaceutical and Biological Products, formulated with physiological saline to prepare a solution of BCG with a concentration of 25mg / mL;
[0037] Lipopolysaccharide (LPS), purchased from SIGMA company, was formulated into a solution with a lipopolysaccharide concentration of 75μg / mL with normal saline;
[0038] Aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) kits, all purchased from Nanjing Jiancheng Institute of Bioengineering;
[0039] Parsleyin B was formulated into suspensions with concentrations of 5, 10, and 20 mg / mL with 0.25% CMC-Na;
[0040] Fifty male ICR mice, weighing 18-25g, were provided by the Experimental Animal Center of the Second Military Medical University. The animals were fed with pellet feed and freely ingested food and wat...
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