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Application of imperatorin in preparing medicament for preventing and treating hepatitis or liver injury

A technology for parsley and chemical liver injury, which is applied in the field of medicine and achieves the effects of high safety, low price and wide sources

Active Publication Date: 2010-12-08
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

], but so far there has been no report about the effect of parsleyin B on preventing and treating viral hepatitis or liver damage

Method used

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  • Application of imperatorin in preparing medicament for preventing and treating hepatitis or liver injury
  • Application of imperatorin in preparing medicament for preventing and treating hepatitis or liver injury
  • Application of imperatorin in preparing medicament for preventing and treating hepatitis or liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1 Preparation of parsleydin B

[0010] Baizhi crude drug decoction pieces (Shanghai Yangpu Traditional Chinese Medicine Decoction Pieces Factory) are crushed and passed through a 120-mesh sieve. Take 1kg of powder and extract 2 times with 5L 95% ethanol under reflux for 2 hours each time. Combine the extracts, concentrate under reduced pressure, recover ethanol, and separate while hot Water layer. After the water layer is left to solidify, it is dissolved with 100ml of 40°C absolute ethanol and left to crystallize. The obtained crystals were recrystallized three times with 10 ml of 95% ethanol at 40°C, and purified with a macroporous adsorption resin to obtain 9.6 mg of parsleyin B. Detected by HPLC, the purity of parsleyin B was 93.2%.

Embodiment 2

[0011] Example 2 The effect of parsleyin B on the expression of hepatitis B virus HBsAg and HBeAg

[0012] 1. Experimental materials

[0013] DMEM medium: product of Gibco, USA, batch number 13200-035;

[0014] Calf serum: product of Gibco, USA, batch number 16000-036;

[0015] HBsAg detection kit: product of Beijing Kewei Clinical Diagnostic Reagent Biotechnology Co., Ltd., batch number: 200706003;

[0016] HBeAg detection kit: Beijing Kewei Clinical Diagnostic Reagent Biotechnology Co., Ltd. product, batch number: 200708008;

[0017] HepG 2.2.15 cells, provided by the Central Laboratory of Shanghai Changhai Hospital, are established by co-transfection of cloned diabody HBV DNA and anti-G418 resistance plasmid into human hepatocarcinoma cell line HepG2 cells, capable of transcribing and translating HBV Gene, and can secrete HBsAg and HBeAg stably into the culture supernatant for a long time. It is currently recognized and commonly used at home and abroad as a cell model for evaluating ...

Embodiment 3

[0034] Example 3 Protective effect of parsleyin B on immune liver injury

[0035] 1. Experimental materials

[0036] BCG lyophilized powder (BCG), purchased from the National Institute for the Control of Pharmaceutical and Biological Products, formulated with physiological saline to prepare a solution of BCG with a concentration of 25mg / mL;

[0037] Lipopolysaccharide (LPS), purchased from SIGMA company, was formulated into a solution with a lipopolysaccharide concentration of 75μg / mL with normal saline;

[0038] Aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) kits, all purchased from Nanjing Jiancheng Institute of Bioengineering;

[0039] Parsleyin B was formulated into suspensions with concentrations of 5, 10, and 20 mg / mL with 0.25% CMC-Na;

[0040] Fifty male ICR mice, weighing 18-25g, were provided by the Experimental Animal Center of the Second Military Medical University. The animals were fed with pellet feed and freely ingested food and wat...

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Abstract

The invention relates to the technical field of medicament, providing a novel application of coumarin compound imperatorin in preparing a medicament for preventing and treating virus hepatitis and resisting immunological liver injury or chemical liver injury. Pharmacological tests prove that the imperatorin has stronger activity of inhibiting hepatitis B surface antigen (HbsAg) and hepatitis B virus e antigen (HbeAg) and resisting the immunological liver injury or the chemical liver injury, so the imperatorin can be used for preparing the medicament for preventing and treating the virus hepatitis and resisting the immunological liver injury or the chemical liver injury. The imperatorin has the advantages of convenient preparation, wide sources, low price and high safety. The invention provides the novel application of the imperatorin, and provides a new medicinal source for preventing and treating the virus hepatitis and resisting the immunological liver injury or the chemical liver injury.

Description

Technical field [0001] The invention relates to the technical field of medicine, and is a new application of the coumarin compound parsleyin B for preparing drugs for preventing and treating viral hepatitis and anti-immunity or anti-chemical liver injury. Background technique [0002] Coumarin compounds are lactone compounds formed by the dehydration of cis-hydroxycinnamic acid. Such compounds are widely distributed in Umbelliferae, Rutaceae, Asteraceae, Leguminosae, and Solanaceae plants, such as Cnidium monnieri, Doraemon, Chinese medicines such as Baizhi, Qianhu, Qinpi, Yinchen, Psoralen, and Xusuizi all contain such ingredients [Luo Xiongming, Qi Shuhua, Yin Hao, Li Minyi, Gao Chenghai, Zhang Si. Research progress on linear furanocoumarin compounds and their pharmacological activities. Research and development of natural products. 2009;21(B05):230-235]. Parsleyin B is one of the coumarin compounds, also known as imperatorin. Its chemical structure is as follows: [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P31/20A61P1/16
Inventor 张黎刘厚佳芮耀诚辛海量李铁军马建丽霍炎王晓黎高申王卓卫立辛王晶赵娜萍潘勇华
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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