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Efficient petroleum hydrocarbon degrading bacterium as well as screening method and application thereof

A technology for petroleum hydrocarbon degrading bacteria and a screening method, which is applied in the field of high-efficiency petroleum hydrocarbon degrading bacteria and its screening, can solve problems such as efficient degradation of difficult petroleum hydrocarbon compounds, and achieves the effects of less negative impact and good development and utilization prospects.

Pending Publication Date: 2021-11-12
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to provide a high-efficiency petroleum hydrocarbon degrading bacteria and its screening method and application to solve the problems that existing bacterial strains are difficult to achieve efficient degradation of petroleum hydrocarbon compounds in a wide range of temperature, pH range and salinity.

Method used

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  • Efficient petroleum hydrocarbon degrading bacterium as well as screening method and application thereof
  • Efficient petroleum hydrocarbon degrading bacterium as well as screening method and application thereof
  • Efficient petroleum hydrocarbon degrading bacterium as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Morphological characteristics Strain

[0053] Single colonies were streaked onto Luria-Bertani solid medium, plates were inverted in the incubator, the culture 28 ℃ 2 days rule colony morphology, were cream-colored, circular colonies were dry, rough surface, opaque, see figure 1 . Strain rod-shaped morphology, see figure 2 .

Embodiment 2

[0055] Screening and Identification of Strains

[0056] Take contaminated soil (sample No. D7, ground 1.7m) mounted in a brown bottle from a depot Shanghai transported back to the laboratory strain screening.

[0057] (1) The basic inorganic salt medium, trace elements and crude mixture was mixed to obtain a liquid medium; wherein the inorganic base in the inorganic salt medium composition contains: 0.2g / L NH 4 Cl, 7.95g / L NaCl, 0.77g / LMgCl 2 · 6H2O, 1.05g / L MgSO 4 · 7h 2 O, 0.076g / L CaCl 2 , 0.22g / L KCl, 0.01g / L NaHCO 3 , 0.026g / L NaBr, 0.25g / L K 2 HPO 4 . Solute component of the trace elements mixture comprises: 0.15g / LZnSO 4 · 7h 2 O, 0.26g / L MnSO 4 · H 2 O, 0.03g / L CoCl 2 · 6h 2 O, 4.5g / L FeSO 4 · 7h 2 O, 0.02g / LNiCl 2 · 6h 2 O, 0.01g / L CuCl 2 , 0.1g / L Na 2 MOO 4 · 2h 2 O, 0.06g / L H 3 BO 3 . The crude oil has a density of 0.8g / cm3, a final concentration of 800 mg / L. Add a volume ratio of inorganic salt medium, trace element solution, crude...

Embodiment 3

[0063] Optimization of petroleum hydrocarbon degradation effect in water body

[0064] After the expansion of Rhodococcus Sp .OBD-3 to 0.8 g / L petroleum hydrocarbons, 8000 r / min was centrifuged for 5 min, and a 1% inorganic salt medium was used to raise the bacteria twice. Connected to 50 ml inorganic salt culture medium in 250ml triangular flask, initial OD 600 0.2. The bacterial liquid was placed in 180 R / min, and the oscillating culture was shaken under the prevention and culture, and the residual concentration of the petroleum hydrocarbons was determined using GC.

[0065] Different substrate concentrations

[0066] Degradation conditions: temperature 28 ° C, pH 6.6, yeast powder 50 mg / L, total salinity 1%; crude oil: 0.5 g / l, 1.0 g / l, 1.5 g / l, 2.0 g / L;

[0067] the result shows: Figure 4 Different substrate concentrations provided in the present invention on petroleum hydrocarbon degradation rate effect of petroleococcus sp. OBD-3 of petroleum hydrocarbon decomp...

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Abstract

The invention relates to an efficient petroleum hydrocarbon degrading bacterium as well as a screening method and application thereof, the petroleum hydrocarbon degrading bacterium is Rhodococcus sp.OBD-3, the Rhodococcus is gram-positive bacterium, the strain form is rod-shaped, the colony form on a Luria-Bertani solid culture medium is regular, cheese-colored and round, and the colony surface is dry, rough and non-transparent. The bacterium can be applied to degradation of organic compounds; and it is identified that when the efficient petroleum hydrocarbon degrading bacterium Rhodococcus sp.OBD-3 is cultured in an aerobic and dark mode at the temperature of 28 DEG C, the pH value of 8.0 and the total salinity of 1%, crude oil with the concentration of 1 g / L can be degraded, the degradation rate within 3 days is 75.8%, and the degradation rate within 7 days is 89.1%. The rhodococcus can efficiently degrade petroleum hydrocarbon under the conditions of a wide temperature range (15-37 DEG C), a pH range (pH 6.0-9.0) and a salinity range (the total salinity is 1%-7%), has small negative influence on the environment, can be applied to biological remediation of general soil or water environment, saline-alkali soil and offshore petroleum pollution, and has good development and utilization prospects.

Description

Technical field [0001] The present invention belongs to the technical field of microbial degradation treatment, relates to a petroleum hydrocarbon degrading bacteria and efficient screening methods and applications. Background technique [0002] With my country's rapid economic development, increasing demand for oil, the oil industry developed rapidly, but the oil pollution is becoming increasingly serious. Oil spill and oil spills occur in oil extraction, refining, processing, transportation and use, soil and water caused serious pollution. Oil pollution affects the permeability of the soil, destroy soil structure, soil affect the growth of microorganisms and plants and reduce the use value of the soil. Oil will form a film on the water surface, thereby reducing the dissolved oxygen in water, serious damage to aquatic ecosystems. On the other hand, the oil contains mostly alkanes, alkanes, cycloalkanes, aromatic hydrocarbons, can enter the body through the food chain and endange...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/32
CPCC12N1/20C02F3/343C02F3/344B09C1/10C02F2101/32
Inventor 崔长征陈欣王辉张磊沈佳敏吕树光金嘉路张峰林匡飞刘勇弟
Owner EAST CHINA UNIV OF SCI & TECH
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