Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of erodium brevifolium element in preparation of Rab5 protein inhibitor

A protein inhibitor, the technology of salicylic acid, which is applied in the field of medicine and achieves the effects of high safety, inhibition of tumor occurrence and development, and good prospects for development and utilization

Pending Publication Date: 2022-03-04
JINAN UNIVERSITY
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no article showing the relationship between geranitin and Rab5 protein, and there is no related patent application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of erodium brevifolium element in preparation of Rab5 protein inhibitor
  • Application of erodium brevifolium element in preparation of Rab5 protein inhibitor
  • Application of erodium brevifolium element in preparation of Rab5 protein inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Binding experiment between geranitin and Rab5 protein

[0041] The present invention expresses the Rab5 gene in the pGEX-4T-1 expression vector, and transforms it into BL-21 expression bacteria, and induces expression with 0.5mM concentration of IPTG (isopropyl-β-D-thiogalactoside) After 4-6 hours, the bacterial cells were collected, and then the recombinant protein was purified by GST affinity chromatography purification column, and then the GST tag was excised to obtain the Rab5 protein.

[0042] The purification process includes the following steps: (1) after mixing the GST affinity chromatography resin (GE; GlutathioneSepharose 4B; 17-0756-01), take 1 mL and put it on the column, and let it stand at room temperature for 30 minutes; (2) use 10 times Column volume ddH 2 O wash the column; (3) equilibrate the column with Binding buffer (1×PBS, pH7.4) of 10 times the column volume; (4) slowly load the collected supernatant sample solution into the chromatogra...

Embodiment 2

[0047] Example 2 Prediction experiment of the binding site between geranitin and Rab5 protein

[0048] Analyzing the binding site between geranidin and Rab5 protein by circular dichroism spectroscopy experiment, specifically including the following steps:

[0049] At a room temperature of 25°C, the CD spectra of 5 μM apo-Rab5 and 6-OA-Rab5 were scanned at a speed of 100 nm / min, using a 0.1 cm quartz cuvette, and the absorption peak wavelength was 190-260 nm. The value collected every 1nm, the protein buffer is 20mM Tric-HCl, pH7.4. The CD spectrum of each protein sample was scanned three times and the average value was calculated, and then the background value of the cuvette and buffer was subtracted to obtain the actual absorption peak of the protein. The calculation of secondary structure content is based on the analysis of experimental data by CDpro software.

[0050] Experimental results such as Figure 4 As shown, through the analysis of circular dichroism spectrum exp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicines, and particularly relates to an application of erodium brachyphyllum element in preparation of a Rab5 protein inhibitor, which proves that the erodium brachyphyllum element and Rab5 can generate interaction by utilizing a plurality of instruments and methods such as fluorescence titration experiment, isothermal titration calorimeter, circular dichroism chromatograph and the like; according to the invention, the molecular docking condition of the erodium brachyphyllum element and the Rab5 protein is also simulated, and a potential interaction site is obtained. Based on the discovery of the action site of the Erodium stephanianum willd as the Rab5 protein, the Erodium stephanianum willd can be used for synthesis of compounds with similar structures and research of drugs, can also be used for screening compounds capable of acting on the action site, and provides a new thought for design of anti-tumor drugs; according to the discovery that the erodium brachyphyllum element can be used as an inhibitor of Rab5 protein, the effect of inhibiting occurrence and development of tumors can be achieved, and the discovery of the invention has a good treatment prospect in screening of Rab5 protein related diseases and related drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of geranidin in the preparation of Rab5 protein inhibitors. Background technique [0002] Geranium brevifolia is a sesquiterpene lactone isolated from the plant Coriander brevifolia[1], and its structural formula is as follows: [0003] [0004] Known studies have shown that geranidin can inhibit the proliferation of various cancer cells, including nasopharyngeal carcinoma [2], lung cancer [3], leukemia [4] and other cancers. In addition, geranidin can also be antibacterial [5] and anti-angiogenesis [6], which is a natural product with biological activity. At present, there is no report on the specific target protein of geranitin. [0005] Rab5 is a member of the Rab protein family and one of the main regulators of early endosomal transport. Rab5 regulates the transport of clathrin-coated vesicles between the plasma membrane and early endosomes ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61K36/28A61P35/00A61P31/12
CPCA61K31/365A61K36/28A61P35/00A61P31/12
Inventor 汪洋张静何庆瑜黄籽佳
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com