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Application of daurisoline in preparation of drugs to prevent and treat lung cancer

A technology of bat puerarin and lung cancer, applied in the field of medicine, can solve the problems of increasing the sensitivity of cancer cells, etc., and achieve the effects of good development and utilization, low price, and small toxic and side effects

Inactive Publication Date: 2019-05-24
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are related reports that bat-guisulin is a natural autophagy inhibitor, which can increase the sensitivity of cancer cells to camptothecin-induced, but for lung cancer, there are no relevant literature and patent reports that bat-guisulin has an effect on the growth of lung cancer cells. Inhibitory

Method used

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  • Application of daurisoline in preparation of drugs to prevent and treat lung cancer
  • Application of daurisoline in preparation of drugs to prevent and treat lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1W

[0018] Example 1 WST-1 cell activity detection

[0019] 1. Cell plating: collect A549 and Hop62 cells (both cells were purchased from ATCC), perform cell counting, spread in 96-well plates according to the amount of 3000 cells per well, and culture overnight.

[0020] 2. Drug treatment: dilute bat-guisurin with DMEM complete medium to the treatment concentration (0, 1.25, 2.5, 5, 10, 20 μM), then replace the original medium in the 96-well plate, and treat for 12, 24 and 48 hours respectively.

[0021] 3. WST-1 detection: according to the dilution ratio of 1:10 (w / v), dilute WST-1 (WST-1 cell proliferation detection kit) with DMEM complete medium to obtain the medium containing WST-1; then The medium containing WST-1 was used to replace the original medium in the 96-well plate, and the medium containing WST-1 was added to wells without cultured cells as a blank control, 100 μL per well. Then put the 96-well plate in a constant temperature incubator at 37°C and incubate for 1 h...

Embodiment 2

[0023] Embodiment 2 clone formation experiment

[0024] 1. Cell plating: collect A549 and Hop62 cells, perform cell counting, spread in 6-well plates according to the amount of 1000 cells per well, and culture overnight.

[0025] 2. Drug treatment: use DMEM complete medium to dilute bat Gesurin to a treatment concentration of 10 μM, then replace the original medium in the 6-well plate, 2 mL per well; at the same time, dilute DMSO (dimethyl sulfoxide) with DMEM complete medium ) to the treatment concentration (10 μM) as the control.

[0026] 3. After two weeks of drug treatment, observe the cell plate under the light to see if there are cloned dots visible to the naked eye.

[0027] 4. When visible cloning dots appear on the cell plate, remove the original medium, wash the remaining medium with PBS buffer, and repeat twice.

[0028] 5. Fixation: Add anhydrous methanol to the 6-well plate, shake the cell plate slowly, and fix at room temperature for 20 minutes.

[0029] 6. St...

Embodiment 3

[0032] Embodiment 3 subcutaneous tumor formation experiment in nude mice

[0033] 1. The animal experiments in this project strictly complied with the guidelines of animal care institutions, and all were approved by the Animal Experiment Ethics Committee of Jinan University.

[0034] 2. The experimental animals used in this project are 6-8 weeks old female nude mice (Balb / c-nu, Experimental Animal Center of Southern Medical University), a total of 15, including 5 in the control group and 10 in the experimental group. Mouse subcutaneous tumor model:

[0035] (1) Cell collection: collect cells for cell counting, and use 1×10 cells per nude mouse 6 The total number of cells was calculated based on the amount of A549 cells, and the cells were resuspended in PBS buffer and mixed with Matrigel (purchased from Guangzhou Sier Biotechnology Co., Ltd., coring) at a volume ratio of 1:1.

[0036] (2) Inoculation: after the mice were anesthetized (evaluate the degree of anesthesia throug...

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Abstract

The invention discloses application of daurisoline in the preparation of drugs to prevent and treat lung cancer. In-vitro and in-vivo experiments show that daurisoline can inhibit the growth and proliferation of lung cancer; daurisoline is a medicinal and edible natural product and natural autophagy retardant, having little toxic and side effect, good safety and low price; therefore, daurisoline can be dissolved in water for oral administration to treat lung cancer; daurisoline has a good development and utilization prospect, and a novel drug choice can be provided for the clinical treatment of lung cancer.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of basalline in the preparation of medicines for preventing and treating lung cancer. Background technique [0002] Lung cancer is one of the most common malignant tumors, and its incidence has been increasing in recent years. Because it is difficult to distinguish lung cancer from benign lung diseases, and the early symptoms of lung cancer are not obvious, most patients have developed to the middle and late stages when they seek treatment, so they often miss the best treatment period, which seriously affects the treatment effect. Currently, lung cancer treatment methods include surgery, radiotherapy, chemotherapy, immunotherapy, and traditional Chinese medicine. However, the resistance of cancer to radiotherapy and chemotherapy leads to postoperative recurrence and poor prognosis of patients, making treatment more difficult, and the commonly used a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4725A61P35/00
Inventor 李斌何庆瑜黄小惠
Owner JINAN UNIVERSITY
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