Paris total saponins extract with tumor metastasis resisting action and pharmaceutical formulation thereof

A technology of anti-tumor metastasis and pharmaceutical preparations, which is applied in the field of total saponin extracts of Papaya and its pharmaceutical preparations. It can solve the problems that the anti-tumor metastasis of saponin extracts has not been found, and achieve the effect of small toxic and side effects

Inactive Publication Date: 2008-07-09
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have shown that the alcohol extract and water extract of Chonglou have inhibitory effects on various tumor cells in vitro, and have obvious inhibitory effects on the growth of animal tra

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] 1. Preparation of total saponin extract of C. chinensis

[0015] After 500g coarse powder of Yunnan Dianzhonglou was passed through a 5-mesh sieve, soaked in 70% and 85% ethanol with a volume ratio of 4 times for 1 hour, and then heated and refluxed for 3 times at a temperature of 85 ° C, 2 hours each time, combined. The extract is concentrated to obtain an extract; the above extract is added to 20% ethanol with a volume ratio of 100 times and dispersed evenly, centrifuged, dissolved after precipitation, centrifuged again, repeated twice, and the supernatant is combined; The supernatant is passed through the macroporous adsorption resin chromatography column, the amount of dry resin used is calculated according to the weight ratio of the extract to 5:1, the flow rate of the upper column is 1~3BV / h, and the eluent is discarded; 60%~80% ethanol is used. Elution, the flow rate is 2-5BV / h, the ethanol eluent is collected, concentrated under reduced pressure and vacuum-dried...

Example Embodiment

[0037] Example 1

[0038] Capsules: (in weight percent)

[0039] 66% of total saponins

[0040] Corn starch 5%

[0041] Dextrin 10%

[0042] Microcrystalline Cellulose 10%

[0043] Calcium Monohydrogen Phosphate 3%

[0044] Micronized silica gel 6%

[0045] The above-mentioned auxiliary materials are wetted with an appropriate amount of 85% ethanol to make soft materials, granulated through a 20-mesh sieve, dried at 40° C. for 1 hour, and then passed through a 20-mesh sieve for granulation, and the obtained granules are poured into capsules to prepare a finished product.

Example Embodiment

[0046] Example 2

[0047] Injection: (by weight percent)

[0048] Chonglou total saponins 98.1%

[0049] Sodium Chloride 0.9%

[0050] Tween-80 1%

[0051] Take water for injection and boil, put in total saponins of Chonglou, stir well, boil for 3-7 minutes, cool to room temperature, filter, add sodium chloride, foot plate, heat to 65 ℃, add Tween-80, stir well, filter, Filter through a 0.22 μm microporous membrane, subpackage, steam sterilize for 1 hour, and fill.

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PUM

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Abstract

The present invention provides a paris total saponin extract for resisting tumor metastasis and a pharmaceutical preparation thereof. The pharmaceutical preparation is composed of paris total saponin extract as the active component and medicinal auxiliary materials; wherein, the weight percentage of paris total saponin extract in the total weight of the pharmaceutical preparation is 60 to 98 percent. The paris total saponin extract for resisting tumor metastasis and the pharmaceutical preparation provided by the present invention have good inhabitation on the metastasis of animal transplanted tumor cells, demonstrated in (1) significant anti-metastasis function for inbred mice and nude mice metastasis model of vaccinated mouse lung adenocarcinoma, human lung cancer, human gastric cancer, human intestinal cancer, human liver cancer and other tumor strains; (2) significant effect on relative albumen metastasis; (3) significant effect on relative gene metastasis and no obvious toxicity on major viscera. In addition, the main active component of the pharmaceutical preparation is a Chinese medicine extract, so compared with chemical drugs, the present invention has the advantage of small toxicity.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to an extract of total saponins of polyphylla with anti-tumor metastasis and a pharmaceutical preparation thereof. Background technique [0002] Malignant tumors are common and frequently-occurring diseases that seriously threaten human health. According to the report of the World Health Organization, among the more than 5 billion people in the world, about 9 million people are newly diagnosed with cancer every year, and 7 million people die due to cancer, accounting for Nearly 20% of the total fatality rate, so the development of anticancer drugs has become a major topic of new drug research in this century. At present, the drug treatment of cancer at home and abroad, especially the molecular targeted therapy, is constantly improving the treatment effect of cancer, but at the same time, it should be noted that many chemotherapeutic drugs may cause a variety of short-term and lon...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61K31/7048A61K9/48A61K9/08A61K9/20A61K9/16A61K9/14A61K9/10A61K9/02A61P35/00A61K125/00
Inventor 高文远颜璐璐满淑丽
Owner TIANJIN UNIV
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