3 results about "Anti metastasis" patented technology

This invention relates to the application of β-sitosterol glycoside in the preparation of products for treating lung cancer. This invention creatively provides a new use for the active monomer β-sitosterol glycoside, demonstrating that β-sitosterol glycoside can significantly inhibit the migration ability of lung cancer cells at doses without cell proliferation inhibitors, clarifying the specificity of its anti-metastatic effect, and laying the foundation for further new drug development of this monomer component. Through an in vivo mouse lung metastasis model, this invention demonstrates that β-sitosterol glycoside effectively inhibits the formation of lung metastases while exhibiting no significant toxic side effects in experimental animals, demonstrating high safety, and providing a new candidate molecule for the development of low-toxicity, highly effective anti-lung cancer metastasis drugs.

The application discloses a kind of construction methods, model and system of cancer metastasis organoid model.The method comprises the following steps: preparing tumor cell spheroid labeled with reporter gene and target organ organoid derived from stem cells respectively;It is placed in culture solution;The preset three-dimensional acoustic field is generated using holographic acoustic tweezers, and at least one tumor cell spheroid is moved and positioned at the preset position of target organ organoid by non-contact precise control, to form initial assembly;The initial assembly is maintained in suspension culture, so that it occurs contact, fusion and invasion, to obtain cancer metastasis organoid model.The application uses acoustic force to realize non-destructive, controllable spatial assembly with micron-level precision, and the model constructed can truly reproduce the interaction of tumor cells and target organ cells in three-dimensional environment, to provide a standardized, quantifiable and scalable in-vitro experimental platform for cancer metastasis mechanism research and anti-metastatic drug screening.