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Method for synthesizing lactosucrose by enzyme method

A technology of lactulose oligosaccharide and enzymatic synthesis, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of no industrial production, etc., and achieve low cost, good development and utilization prospects, and simple process Effect

Inactive Publication Date: 2010-07-14
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestically, preliminary research has been done on lactulose-oligosaccharides, but there is no report on industrial production, so it has broad application prospects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Prepare lactose and sucrose (1:1) with PBS solution to make a mixed sugar solution with a final concentration of 30% (m / v) and a pH of 7.5, mix the mixed sugar solution with the crude enzyme solution, and mix the sugar solution with the crude enzyme solution The ratio was 3:4, and after mixing well, the mixture was reacted at 35° C. for 16 hours. After the reaction was completed, the enzyme was inactivated at 100° C. for 10 minutes, centrifuged at 4500 r / min for 25 minutes to remove protein, and a 21.45% lactulose-oligosaccharide solution was obtained.

Embodiment 2

[0016] Prepare lactose and sucrose (1:1) with PBS solution to make a mixed sugar solution with a final concentration of 40% (m / v) and a pH of 6.5, mix the mixed sugar solution with the crude enzyme solution, and mix the sugar solution with the crude enzyme solution The ratio of the mixture is 3:2, and after thorough mixing, the mixture is reacted at 40° C. for 24 hours. After the reaction was completed, the enzyme was inactivated at 100° C. for 10 minutes, and centrifuged at 4500 r / min for 25 minutes to remove protein to obtain a 19.21% lactulose-oligosaccharide solution.

Embodiment 3

[0018] Prepare lactose and sucrose (1:1) with PBS solution to make a mixed sugar solution with a final concentration of 40% (m / v) and a pH of 7.0, mix the mixed sugar solution with the crude enzyme solution, and mix the sugar solution with the crude enzyme solution The ratio was 1:1, and after mixing well, the mixture was reacted at 30° C. for 16 hours. After the reaction was completed, the enzyme was inactivated at 100° C. for 10 minutes, and centrifuged at 4500 r / min for 25 minutes to remove protein to obtain a 19.75% lactulose-oligosaccharide solution.

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Abstract

The invention relates to a method for synthesizing lactosucrose by the enzyme method, which is characterized by comprising the following steps: (1), equivalent amounts of lactose and sucrose are prepared into mixed sugar with the final concentration of 30%-60% and pH value of 6.5-7.5 by using a PBS solution; (2) the mixed sugar is mixed with crude enzyme containing Beta-fructofuranosidase, and the volume ratio of the mixed sugar to the crude enzyme is 3:1-3:5; and (3) the mixture reacts at a temperature of 30 DEG C to 45 DEG C for 12-32 hours, and after the completion of enzymatic reaction, the enzyme inactivation is carried out at a temperature of 80 DEG C to 100 DEG C for 10 to 20 minutes, the centrifuging is carried out at a speed of 3000-6000r / min for 20-40 minutes, and protein is removed to obtain a lactosucrose solution with the content of 15% to 28%. The invention has the advantages of simple process, low cost and the like, and the lactosucrose yield is further improved.

Description

technical field [0001] The invention relates to a method for enzymatically synthesizing lactulose-oligosaccharides, that is, a method for producing fructosidase produced by strains (Arthrobacter sp10138) to produce lactulose-oligosaccharides. Background technique [0002] Lacto-oligosaccharide (lactosucrose, LS) is a kind of functional oligosaccharide, the chemical name is O-β-D-galactopyranosyl-(1→2)-β-D-fructofuranoside, which is made of sucrose and lactose , produced by the action of β-fructofuranosidase produced by Arthrobacter. First, sucrose is first catalytically hydrolyzed into glucose and fructose, and then the decomposed fructosyl is preferentially transferred to the hydroxyl group at the C1 position of the reducing end of lactose, thereby synthesizing 4-β-D-galactosyl sucrose, namely lactose oligosaccharides . This sugar is a non-reducing oligosaccharide composed of three monosaccharides, whose structure viewed from one side is lactose followed by a fructosyl gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12R1/06
Inventor 阮征廖春龙印遇龙文红艳代志凯
Owner NANCHANG UNIV
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