Method for concurrently improving spirulina biomass and polysaccharide yield

A technology of spirulina and biomass, applied in the field of spirulina to accumulate biomass and polysaccharides, can solve the problems of affecting cell growth and photosynthetic carbon fixation efficiency, the yield of polysaccharides is not significantly improved, and the production of spirulina polysaccharides is affected. Good sustainability, promotion of industrial production, and low production cost

Inactive Publication Date: 2016-06-08
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, under nutrient-rich conditions, the polysaccharide content of Spirulina is very small, accounting for less than 8% of the dry cell weight, which seriously affects the yield of polysaccharides, increases the cost of subsequent processing and extraction, and makes it difficult for industrial production
On the other hand, under stress conditions, microalgal cells tend to accumulate sugars and oils, but at the same time the synthesis of proteins is hindered, which will affect the growth of cells and the efficiency of photosynthetic carbon fixation, reduce the yield of biomass, and thus make polysaccharides The yield did not increase significantly

Method used

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  • Method for concurrently improving spirulina biomass and polysaccharide yield
  • Method for concurrently improving spirulina biomass and polysaccharide yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The accumulation of biomass and polysaccharides in Spirulina was investigated under P-limited and S-limited conditions.

[0022] The algae cultivation and polysaccharide accumulation processes were carried out in a 500mL cylindrical bubbling photobioreactor (glass material, diameter 50mm, height 400mm).

[0023] 1) Cultivation of algal species

[0024] Spirulina cells are cultured in a nutrient-rich medium (Zarrouk). The medium is prepared with deionized water, and each liter of water contains: NaHCO316.8g, K2HPO40.5g, NaNO32.5g, K2SO41g, NaCl1g, MgSO4 7H2O0.2g, CaCl2·2H2O0.04g, FeSO4·7H2O0.01g, H3BO32.86mg, MnCl2·4H2O1.86mg, ZnSO4·7H2O0.22mg, Na2MoO4·2H2O0.39mg, CuSO4·5H2O0.08mg. The initial inoculation density OD560 is 0.5, the culture temperature is 30±2°C, continuous single-row illumination, the light intensity is 200 μmolm–2s–1, and the air flow rate is 0.4vvm.

[0025] 2) Simultaneous accumulation of algal cell biomass and polysaccharides

[0026] Centrifuge th...

Embodiment 2

[0034] To investigate the effect of N limitation on biomass and polysaccharide accumulation of Spirulina in small-scale indoor cultivation.

[0035] The cultivation of the algae species is the same as that of the algae species in Example 1. The polysaccharide accumulation process was carried out in a 6L plastic square basin with a water depth of 8cm.

[0036] Centrifuge the cells cultivated to an OD560 of 3 at 4000 rpm for 5 min, discard the supernatant, wash the algae mud once with NaNO3-free Zarrouk medium (Zarrouk-N) and resuspend it in Zarrouk-N, adjust the cell density to 0.5 for OD560, Inoculate in a 6L plastic square basin, and stir the algae solution with a submersible pump with a power of 5W. The culture temperature is 23±2°C, under natural conditions, and the maximum light intensity on the surface of the algae liquid does not exceed 84.5 μmolm–2s–1 for 90% of the time.

[0037] Nutrient-rich conditions (+N): add NaNO32.5g per liter of algae liquid.

[0038] N limi...

Embodiment 3

[0042] Large-scale cultivation of Spirulina using N limitation in an outdoor racetrack pond for enhanced biomass and polysaccharide yields.

[0043] The process of algae cultivation and polysaccharide accumulation was carried out in an outdoor 1-acre track pool with a water depth of 30 cm and a water velocity of 20 cm / s.

[0044] 1) Cultivation of algal species

[0045] Spirulina cells are cultured in nutrient-rich natural mother liquor alkali medium (MYJ), and the culture medium is prepared with underground fresh water. Each liter of water contains: 6g of mother liquor alkali (containing about 3.4g of NaHCO3) produced by Ordos plateau natural alkali lake, NaNO31g , KCl0.5g, H3PO40.12g, FeSO4·7H2O0.01g, MgSO4·7H2O0.01g. The initial inoculation density OD560 was 0.3, the culture temperature was 32±5°C, the natural conditions were lighted, and the maximum light intensity on the surface of the algae liquid was 3000 μmolm–2s–1.

[0046] 2) Simultaneous accumulation of algal cell...

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Abstract

The present invention relates to biomass and polysaccharide accumulation through spirulina culture, specifically to spirulina culture using the control of the addition of a nutritional salt so as to concurrently accumulate biomass and polysaccharides. According to the present invention, spirulina cells cultured to achieve a exponential growth phase are transferred into a nutrition limiting culture medium, natural illumination or artificial illumination is performed, culture is performed to achieve a stable phase, and the spirulina cells are harvested, wherein the biomass yield is 1-4 times the biomass yield of the culture under the rich nutrition condition, the polysaccharides yield is increased by 0.8-20 times compared to the culture under the rich nutrition condition, and the polysaccharide content achieves 45-80% of the spirulina dry weight, and is increased by 2-6 times compared to the culture under the rich nutrition condition; and the contradiction that the spirulina biomass and the polysaccharide cannot be concurrently accumulated is solved, the rapid and efficient spirulina polysaccharide production is achieved, the advantages of low nutrition salt consumption, low production cost, high polysaccharide content and the like are provided, the downstream processing operations are easily simplified, and the industrial production of the spirulina polysaccharide can be promoted.

Description

technical field [0001] The invention relates to accumulating biomass and polysaccharides by cultivating spirulina, in particular to cultivating spirulina by controlling the addition of nutrient salts and adopting appropriate inoculation density, light, temperature and pH value to simultaneously accumulate biomass and polysaccharides. It can be used in the cultivation of spirulina and the production of polysaccharides, as well as related fields such as food, medicine, and energy using spirulina polysaccharides as raw materials. Background technique [0002] Spirulina is a multicellular filamentous cyanobacteria (BlueGreenAlgae), also known as cyanobacteria (Cyanobacteria), belongs to Cyanophyta, Cyanophyceae, Oscillatoriales, Osciallatoriaceae, Arthrospira is mainly distributed in freshwater or saline-alkaline lakes in tropical and subtropical regions. Spirulina is rich in protein, unsaturated fatty acids, pigments, polysaccharides, vitamins and trace elements. It is conside...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 薛松姚长洪陆洪斌曹旭鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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