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Production method of grifolan selenium compound

A technique for Grifola frondosa polysaccharide, production method, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Active Publication Date: 2014-05-28
GUANGDONG SHENGYUAN ZHONGTIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The new production method must be supported by a new process. Using the mutagenic strain adopted in the present invention, fermentation is carried out on rice bran bran plus selenium compound medium without enzymatic hydrolysis of plant fibers, and the process of high-yield Grifola frondosa polysaccharide-selenium complex It is a brand-new process, and this kind of research has not been reported publicly

Method used

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  • Production method of grifolan selenium compound

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Effect test

Embodiment 1

[0018] Grifola frondosa strain obtained by mutagenesis Grifola sp. JSU 10-2 strain. The rice bran and bran were leached at 90°C for 2.5 hours, and the treated liquid was used as a medium for fermentation. The amount of rice bran used was 30g / L medium, the amount of bran used was 30g / L medium, and the amount of selenium added 5mg / L medium, add potassium dihydrogen phosphate 1.0g / L medium, magnesium sulfate 0.5g / L medium, pH is natural, the sample volume of the shake flask is 40% volume, the inoculum size is 10%, and the culture temperature is 26 ℃, rotation speed 150r / min, incubation time 7d. The mycelia obtained from liquid culture were centrifuged and washed 3 times with distilled water to remove the culture medium adhering to the surface of the mycelium, and then put into a blast drying oven and dried to constant weight at 60°C. The dry weight of mycelium was obtained by weighing. Add a certain volume of distilled water to the dried mycelia, after grinding, extract in a...

Embodiment 2

[0020] Grifola frondosa strain obtained by mutagenesis Grifola sp. JSU 10-2 strain. The rice bran and bran were leached at 98°C for 3 hours, and the treated solution was obtained as a medium for fermentation. The amount of rice bran used was 120g / L medium, the amount of bran used was 120g / L medium, and the amount of selenium added was 10mg / L medium, add potassium dihydrogen phosphate 1.5g / L medium, magnesium sulfate 0.8g / L medium, pH is natural, the sample volume of the shake flask is 40% volume, the inoculum volume is 10%, and the culture temperature is 28°C , rotation speed 180r / min, culture time 9d. The mycelia obtained from liquid culture were centrifuged and washed 3 times with distilled water to remove the culture medium adhering to the surface of the mycelium, and then put into a blast drying oven and dried to constant weight at 60°C. The dry weight of mycelium was obtained by weighing. Add a certain volume of distilled water to the dried mycelia, after grinding, e...

Embodiment 3

[0022] Grifola frondosa strain obtained by mutagenesis Grifola sp. JSU 10-2 strain. The rice bran and bran were leached at 95°C for 2.8 hours, and the treated solution was used as a medium for fermentation. The amount of rice bran used was 120g / L medium, the amount of bran used was 50g / L medium, and the amount of selenium added For 10mg / L culture medium, add potassium dihydrogen phosphate 1.5g / L culture medium, magnesium sulfate 0.8 g / L culture medium, pH is natural, the sample volume of shaking flask is 40% volume, the inoculum size is 10%, and the culture temperature is 28 ℃, rotation speed 150r / min, incubation time 7d. The mycelia obtained from liquid culture were centrifuged and washed 3 times with distilled water to remove the culture medium adhering to the surface of the mycelium, and then put into a blast drying oven and dried to constant weight at 60°C. The dry weight of mycelium was obtained by weighing. Add a certain volume of distilled water to the dried myceli...

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Abstract

The invention relates to a production method of a grifolan selenium compound, and relates to the food microbiological application technology field. The method comprises the steps that: rice bran and wheat bran are leached in water with a temperature of 90 DEG C to 98 DEG C for 2.5-3h under normal pressure; residuals are removed, and a juice is obtained; the obtained process liquid is used as a culture media for fermentation, wherein the rice bran application amount is 30-120g / L culture media, the wheat bran application amount is 30-120g / L culture media, an addition amount of selenium is 5-10mg / L culture media, an addition amount of potassium dihydrogen phosphate is 1.0-1.5g / L, an addition amount of magnesium sulfate is 0.5-0.80g / L, and a pH value is natural; mycelium obtained by liquid culturing is subjected to centrifugal separation, and is washed by using distilled water; the mycelium is dried to constant weight, such that the mycelium is obtained; the mycelium is subjected to conventional water extraction, deproteinization, ethanol precipitation, and lyophilization, such that a intracellular polysaccharide selenium compound can be obtained. With the method provided by the invention, the production of grifolan selenium compound by using cheap raw materials is better realized.

Description

technical field [0001] The invention relates to the technical field of food microorganism application, in particular to the use of a grifola frondosa mutagenic strain suitable for growth on a culture medium in which rice bran and bran composites are carbon and nitrogen sources, to ferment and produce high-yield Grifola frondosa polysaccharide-selenium compound method of production. Background technique [0002] Scientific research at home and abroad and the domestic market have shown that edible fungus polysaccharides can enhance human immunity and assist in the treatment of tumors and other diseases, and have become an auxiliary product for tumor biotherapy. Domestic medicinal products made from the fruiting bodies of edible fungi include Ganoderma lucidum capsules from Wuhu, Anhui, and Grifola frondosa capsules from Qingyuan, Zhejiang. Non-pharmaceutical health products made from edible fungus extracts are also sold. Internationally, New Zealand produces "GanoPoly" polysa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 刘伟民郭春梅陈钧沈国栋徐立平张红印张建任晓锋刘丽丽赵莉崔凤杰张志才赵杰文
Owner GUANGDONG SHENGYUAN ZHONGTIAN BIOTECH CO LTD
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