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A method for increasing the yield of Spirulina biomass and polysaccharides simultaneously

A technology of spirulina and biomass, applied in the field of spirulina to accumulate biomass and polysaccharides, can solve the problems of affecting cell growth and photosynthetic carbon fixation efficiency, the yield of polysaccharides is not significantly improved, and the production of spirulina polysaccharides is affected. Good sustainability, promotion of industrial production, and low production cost

Inactive Publication Date: 2019-05-21
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, under nutrient-rich conditions, the polysaccharide content of Spirulina is very small, accounting for less than 8% of the dry cell weight, which seriously affects the yield of polysaccharides, increases the cost of subsequent processing and extraction, and makes it difficult for industrial production
On the other hand, under stress conditions, microalgal cells tend to accumulate sugars and oils, but at the same time the synthesis of proteins is hindered, which will affect the growth of cells and the efficiency of photosynthetic carbon fixation, reduce the yield of biomass, and thus make polysaccharides The yield did not increase significantly

Method used

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  • A method for increasing the yield of Spirulina biomass and polysaccharides simultaneously
  • A method for increasing the yield of Spirulina biomass and polysaccharides simultaneously

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The accumulation of biomass and polysaccharides in Spirulina was investigated under P-limited and S-limited conditions.

[0022] The algae cultivation and polysaccharide accumulation processes were carried out in a 500mL cylindrical bubbling photobioreactor (glass material, diameter 50mm, height 400mm).

[0023] 1) Cultivation of algal species

[0024] Spirulina cells are cultured in a nutrient-rich medium (Zarrouk) prepared with deionized water containing: NaHCO per liter of water3 16.8g, K 2 HPO 4 0.5g, NaNO 3 2.5g, K 2 SO 4 1g, NaCl 1g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.04g, FeSO 4 ·7H 2 O 0.01g, H 3 BO 3 2.86mg, MnCl 2 4H 2 O 1.86mg, ZnSO 4 ·7H 2 O0.22mg, Na 2 MoO 4 2H 2 O 0.39mg, CuSO 4 ·5H 2 O 0.08 mg. Initial inoculation density OD 560 0.5, culture temperature 30±2℃, continuous single-row light, light intensity 200μmol m –2 the s –1 , into the air flow rate of 0.4vvm.

[0025] 2) Simultaneous accumulation of algal cell biomass and...

Embodiment 2

[0034] To investigate the effect of N limitation on biomass and polysaccharide accumulation of Spirulina in small-scale indoor cultivation.

[0035] The cultivation of the algae species is the same as that of the algae species in Example 1. The polysaccharide accumulation process was carried out in a 6L plastic square basin with a water depth of 8cm.

[0036] Grow to OD 560 3 cells were centrifuged at 4000rpm for 5min, the supernatant was discarded, and the algal mud was used without NaNO 3 Zarrouk medium (Zarrouk-N) was washed once and then resuspended in Zarrouk-N to adjust the cell density OD 560 0.5, inoculated in a 6L plastic square basin, and stirred the algae solution with a submersible pump with a power of 5W. The culture temperature is 23±2°C, under natural conditions, the maximum light intensity on the surface of the algae liquid does not exceed 84.5 μmol m for 90% of the time –2 the s –1 .

[0037] Nutrient-rich conditions (+N): add NaNO to each liter of algae...

Embodiment 3

[0042] Large-scale cultivation of Spirulina using N limitation in an outdoor racetrack pond for enhanced biomass and polysaccharide yields.

[0043] The process of algae cultivation and polysaccharide accumulation was carried out in an outdoor 1-acre track pool with a water depth of 30 cm and a water flow velocity of 20 cm / s.

[0044] 1) Cultivation of algal species

[0045] Spirulina cells are cultured in nutrient-rich natural mother liquor alkali medium (MYJ), and the culture medium is prepared with underground fresh water, and each liter of water contains: 6g of mother liquor alkali (containing NaHCO 3 about 3.4g), NaNO 3 1g, KCl0.5g, H 3 PO 4 0.12g, FeSO 4 ·7H 2 O0.01g, MgSO 4 ·7H 2 O 0.01 g. Initial inoculation density OD 560 0.3, the culture temperature is 32±5°C, the natural conditions are illuminated, and the maximum light intensity on the surface of the algae liquid is 3000 μmol m –2 the s –1 .

[0046] 2) Simultaneous accumulation of algal cell biomass ...

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Abstract

The invention relates to the cultivation and accumulation of biomass and polysaccharides of spirulina, in particular to the cultivation of spirulina by controlling the addition of nutrient salts so that the biomass and polysaccharides can be accumulated simultaneously. The spirulina cells cultivated to the exponential growth phase are transferred to the nutrient-limited medium, with natural light or artificial light, and the algae cells are harvested after being cultivated to the stable phase. The yield is 0.8-20 times higher than that under the condition of rich nutrition, and the polysaccharide content reaches 45%-80% of the dry weight of algae, which is 2-6 times higher than that under the condition of rich nutrition. The invention solves the contradiction that spirulina biomass and polysaccharides cannot be accumulated at the same time, realizes the rapid and efficient production of spirulina polysaccharides, has the advantages of less nutrient salt consumption, low production cost, high polysaccharide content, etc., and is conducive to simplifying downstream processing operations and promoting spirulina Industrial production of algal polysaccharides.

Description

technical field [0001] The invention relates to accumulating biomass and polysaccharides by cultivating spirulina, in particular to cultivating spirulina by controlling the addition of nutrient salts and adopting appropriate inoculation density, light, temperature and pH value to simultaneously accumulate biomass and polysaccharides. It can be used in the cultivation of spirulina and the production of polysaccharides, as well as related fields such as food, medicine, and energy using spirulina polysaccharides as raw materials. Background technique [0002] Spirulina is a kind of multicellular filamentous cyanobacteria (Blue Green Algae), also known as cyanobacteria (Cyanobacteria), belongs to Cyanophyta (Cyanophyta), Cyanophyceae (Cyanophyceae), Oscillatoriales (Oscillatoriales), Oscillatoriaceae (Osciallatoriaceae) ), Arthrospira, mainly distributed in freshwater or saline-alkaline lakes in tropical and subtropical regions. Spirulina is rich in protein, unsaturated fatty a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 薛松姚长洪陆洪斌曹旭鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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