Porphyridium extracellular polysaccharide adsorbent and preparation method thereof

A technology of extracellular polysaccharides and Porphyridium, applied in chemical instruments and methods, adsorption water/sewage treatment, other chemical processes, etc., can solve the problems of small adsorption capacity, poor regeneration ability, poor stress resistance, etc., and achieve adsorption capacity Larger, less contamination residues, and easier elution

Inactive Publication Date: 2016-06-15
TIANJIN SANSHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, low yield, small adsorption capacity, poor stress resistance and poor regeneration ability are still the dominant factors restricting its large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Separation and purification of exopolysaccharide from Porphyridium algae:

[0036] 1) Take fresh Porphyridum algae and mix with distilled water or phosphate buffer solution, and perform ultrasonic crushing. The pH value of the phosphate buffer solution is 7.5, and the concentration is 60mmol / L;

[0037] 2) centrifuging the crushed liquid obtained in step 1), discarding the precipitate, and taking the supernatant;

[0038] 3) deproteinize the supernatant obtained in step 2);

[0039] 4) Add 2 times the volume of ethanol to the deproteinized supernatant obtained in step 3) for precipitation, collect the precipitate by centrifugation, wash with acetone, and dry to obtain crude polysaccharide;

[0040] 5) Dissolve the crude polysaccharide obtained in step 4) in distilled water to make a saturated solution, centrifuge and discard the precipitate, apply the supernatant to a DEAE-Sepharose FastFlow column, use Tris-HCl as the eluent, and perform NaCl gradient elution, Ph...

Embodiment 2

[0044] (1) Separation and purification of exopolysaccharide from Porphyridium algae:

[0045]1) Mix fresh Porphyridum algae with distilled water or phosphate buffer solution, and perform ultrasonic crushing. The pH value of the phosphate buffer solution is 7.5, and the concentration is 100mmol / L;

[0046] 2) centrifuging the crushed liquid obtained in step 1), discarding the precipitate, and taking the supernatant;

[0047] 3) deproteinize the supernatant obtained in step 2);

[0048] 4) adding 3 times the volume of ethanol to the deproteinized supernatant obtained in step 3) for precipitation, centrifuging to collect the precipitate, washing with acetone, and drying to obtain crude polysaccharide;

[0049] 5) Dissolve the crude polysaccharide obtained in step 4) in tris-hydrochloric acid buffer to make a saturated solution, centrifuge and discard the precipitate, put the supernatant on a DEAE-Sepharose FastFlow column, wash with Tris-HCl Deliquidation, NaCl gradient elution...

Embodiment 3

[0053] (1) Separation and purification of exopolysaccharide from Porphyridium algae:

[0054] 1) Take fresh Porphyridum algae and mix with distilled water or phosphate buffer solution, and perform ultrasonic crushing. The pH value of the phosphate buffer solution is 7.5, and the concentration is 40mmol / L;

[0055] 2) centrifuging the crushed liquid obtained in step 1), discarding the precipitate, and taking the supernatant;

[0056] 3) deproteinize the supernatant obtained in step 2);

[0057] 4) Add 2.5 times the volume of ethanol to the deproteinized supernatant obtained in step 3) for precipitation, collect the precipitate by centrifugation, wash with acetone, and dry to obtain crude polysaccharide;

[0058] 5) Dissolve the crude polysaccharide obtained in step 4) in tris-hydrochloric acid buffer to make a saturated solution, centrifuge and discard the precipitate, put the supernatant on a DEAE-Sepharose FastFlow column, wash with Tris-HCl Deliquidation, NaCl gradient elu...

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PUM

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Abstract

The invention relates to a porphyridium extracellular polysaccharide adsorbent and a preparation method thereof. The method comprises the steps of porphyridium extracellular polysaccharide separation purification treatment, porphyridium extracellular polysaccharide sulfonation treatment and porphyridium extracellular polysaccharide immobilization treatment. The adsorbent prepared with the method can selectively adsorb heavy metal ions and precious metal ions in water body. The porphyridium extracellular polysaccharide adsorbent has the advantages of fast reaction, high adsorbent capacity, high removal rate, and easy elution. The adsorbent can be repeatedly used, such that secondary pollution to the environment can be reduced. Therefore, the adsorbent is environment-friendly. With the adsorbent, ecological risks caused by pollution residue and species introduction are reduced. The adsorbent and the method are suitable for industrialized productions.

Description

technical field [0001] The invention relates to the field of biochemical adsorbents, in particular to a method for preparing an adsorbent for extracellular polysaccharides from Porphylococcus algae. Background technique [0002] With social development and technological progress, new requirements are put forward for separation materials: large adsorption capacity, fast adsorption speed, and large selective adsorption capacity, which can selectively adsorb and separate the required substances or harmful substances in the solution. , easy desorption, can be used repeatedly; cheap, durable, wide source; can be used in various forms; simple separation technology, less energy consumption, many varieties, different varieties can be used according to different requirements. [0003] In view of the above requirements, biosorbent materials have entered people's field of vision with their unique advantages, and many scholars at home and abroad have conducted extensive and in-depth res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30C02F1/28C08B37/00
Inventor 季延滨李涛孙学亮
Owner TIANJIN SANSHENG BIOTECH CO LTD
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