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Additive for liquid nutrient medium for Irpex lacteus fermentation

A technology of leucococcus rhizoma and liquid culture medium, which is applied in application, fertilizer mixture, fertilization device and other directions, can solve the problems of long fermentation period and low content of active ingredients, and achieves promoting accumulation, increasing polysaccharide yield and shortening cultivation period. Effect

Inactive Publication Date: 2012-09-26
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing medium has a long fermentation cycle and low content of active ingredients

Method used

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  • Additive for liquid nutrient medium for Irpex lacteus fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 20g of glucose, 10g of peptone, 10g of yeast extract powder, 0.5g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, and 0.1g of vitamin B1, add water to 1 liter, and divide them into 250mL conical flasks. 100mL per bottle, sterilized at 121°C for 20min. After the medium is cooled, add 5mL of the prepared R. albicans suspension into the medium according to the method of aseptic operation, then add 8g of sterilized calcium lignosulfonate, 5g of natural borneol, 26°C, 150 rpm Shake culture per minute for 67 hours.

Embodiment 2

[0023] Take 20g of glucose, 10g of peptone, 10g of yeast extract powder, 0.5g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, and 0.1g of vitamin B1, add water to 1 liter, and divide them into 250mL conical flasks. 100mL per bottle, sterilized at 121°C for 20min. After the medium is cooled, add 5mL of the prepared R. albicans suspension into the medium according to the method of aseptic operation, then add 5g of sterilized calcium lignosulfonate, 3g of natural borneol, 26°C, 150 rpm Shake culture per minute for 67 hours.

Embodiment 3

[0025] Take 29.34g of lactose, 17.13g of yeast extract powder, 0.64g of magnesium sulfate, 0.30g of potassium dihydrogen phosphate, 0.000585g of sodium chloride, 0.00090g of calcium chloride, 0.50g of potassium nitrate, 0.10g of vitamin B1, and add water to 1 liter. Divide into 250mL Erlenmeyer flasks, the filling volume is 100mL per bottle, sterilize at 121°C for 20min. After the medium is cooled, add 5 mL of the prepared R. albicans suspension into the medium according to the method of aseptic operation, then add 10 g of sterilized calcium lignosulfonate, 3 g of natural borneol, 26 ° C, 150 rpm Shake culture per minute for 67 hours.

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Abstract

The invention provides an additive for a liquid nutrient medium for Irpex lacteus fermentation, relating to formula optimization of a liquid nutrient medium for Irpex lacteus fermentation. Calcium lignosulphonate and natural borneol are added into the broad liquid nutrient medium to enhance the transport efficiency of Irpex lacteus cells, and promote the absorption and utilization of the Irpex lacteus for nutrient substances, thereby enhancing the yield of the cellular metabolism products adenosin and polysaccharide and the dry weight of hypha. The total adenosin content is up to 0.135-0.149 g / L, which is enhanced by 8.2% on average as compared with the liquid nutrient medium without the additive; the total polysaccharide content is up to 2...381-2.605 g / L, which is enhanced by 11.0% on average as compared with the liquid nutrient medium without the additive; and the dry weight of hypha is up to 15.176-15.320 g / L, which is enhanced by 9.1% on average as compared with the liquid nutrient medium without the additive. The additive can be used as a liquid nutrient medium additive for industrially fermenting Irpex lacteus.

Description

technical field [0001] The invention provides an additive for a liquid culture medium for the fermentation of R. albicans, which relates to the optimization of the formula of the fermentation liquid culture medium for R. albicans, which can effectively improve the production of mycelium and the content of some active ingredients during liquid fermentation, and belongs to medicinal use. Field of fungal fermentation technology. Background technique [0002] Albicans albicans is a medicinal fungus with high medical value. It has good curative effect in treating oliguria, edema, low back pain, elevated blood pressure and other diseases, and it is the main raw material source of some kidney disease drugs. [0003] With the continuous deepening of the development of A. albicans, it has been found that it has anti-inflammatory, immune regulation, enhanced mononuclear macrophage system function, enhanced cellular immune function, promoted cytokine production, and enhanced humoral i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 滕利荣汤海峰逯家辉孟庆繁权宇彤王德利邵妍姜丹孟令军孟威
Owner JILIN UNIV
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