Development and application of co-colonization type multifunctional complex microbial inoculant based on plant rhizosphere biofilm

A compound bacterial agent and biofilm technology, which is applied in the field of agricultural microorganisms, can solve the problems of restricting the colonization ability of growth-promoting bacteria, restricting the development of microbial fertilizers, and affecting the stability of the field effects of microbial fertilizers.

Active Publication Date: 2021-04-16
NANJING AGRICULTURAL UNIVERSITY
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After the microbial fertilizer is applied to the soil, on the one hand, it needs to face the problem of living environment pressure caused by abiotic factors such as soil physical and chemical properties, and on the other hand, it also faces problems such...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Development and application of co-colonization type multifunctional complex microbial inoculant based on plant rhizosphere biofilm
  • Development and application of co-colonization type multifunctional complex microbial inoculant based on plant rhizosphere biofilm
  • Development and application of co-colonization type multifunctional complex microbial inoculant based on plant rhizosphere biofilm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] 1. Isolation and identification of strains

[0030] The cucumber rhizosphere growth-promoting Bacillus SQR9 has a strain preservation number of CGMCC No. 5808, which was isolated in a laboratory earlier. Pseudomonas stutzeri RFPse-2 was screened from the cucumber rhizosphere inoculated with the strain SQR9: the cucumber root system was removed from the soil, and the soil adhered to the root surface was collected. Take 0.1g of rhizosphere soil and add it to a 1.5mL centrifuge tube filled with 1mL 0.9% sodium chloride and glass beads, shake at the maximum speed for 20 minutes to form a soil suspension, and absorb 10 -6 ,10 -7 ,10 -8 0.1 mL of the soil suspension with a dilution factor of 0.1 mL was spread on the TSB plate, and each concentration was repeated three times. After culturing at 30°C for 2-7 days, the colonies with different shapes were selected and purified on the plate, and inoculated into the TSB liquid medium for 18- 24h, stored in a 15% glycerol tube at...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides development and application of a co-colonization type complex microbial inoculant based on a plant rhizosphere biofilm. The plant probiotic complex microbial inoculant is composed of bacillus amyloliquefaciens SQR9 and pseudomonas stutzeri XL272. The bacterial strain XL272 is separated from the rhizosphere of cucumber inoculated with a single bacterial strain SQR9, and can be metabolized and mutually nourished with the bacterial strain SQR9 to form the biofilm, and the biomass of the biofilm formed by the bacterial strain XL272 and the bacterial strain SQR9 is obviously higher than the biomass of a biofilm formed by a single bacterium. Fluorescently labeled strains are co-inoculated to the rhizosphere of a plant, and the strains SQR9 (green) and XL272 (purple) can be co-colonized and jointly generate a synergistic probiotic effect on the plant. The plant probiotic complex microbial inoculant not only can significantly promote cucumber growth, but also can help plants to resist salt in soil with a salt content of 0.3%.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to the development and application of a plant growth-promoting compound bacterial agent based on rhizosphere biofilm co-colonization. Background technique [0002] Composite microbial bacterial agent is a bacterial agent product composed of two or more microorganisms. The essence of the composite microbial agent is artificially designed synthetic microbial ecology, which is mainly aimed at specific purposes, using rational design of microbial combinations or ecosystems with new functions and behaviors. Compared with single microorganisms and natural microbial groups, synthetic microbial communities have the advantages of low complexity, high controllability, and good stability. The research on the application of synthetic microbial communities, that is, compound bacterial agents, in agriculture is still in its infancy. How to use existing ecological theories to rationally d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A01G22/05A01G7/06C12R1/07C12R1/38
CPCY02A40/10
Inventor 徐志辉孙新丽张瑞福解继驭邵佳慧沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap