Development and application of co-colonization type multifunctional complex microbial inoculant based on plant rhizosphere biofilm

Abstract

The invention provides development and application of a co-colonization type complex microbial inoculant based on a plant rhizosphere biofilm. The plant probiotic complex microbial inoculant is composed of bacillus amyloliquefaciens SQR9 and pseudomonas stutzeri XL272. The bacterial strain XL272 is separated from the rhizosphere of cucumber inoculated with a single bacterial strain SQR9, and can be metabolized and mutually nourished with the bacterial strain SQR9 to form the biofilm, and the biomass of the biofilm formed by the bacterial strain XL272 and the bacterial strain SQR9 is obviously higher than the biomass of a biofilm formed by a single bacterium. Fluorescently labeled strains are co-inoculated to the rhizosphere of a plant, and the strains SQR9 (green) and XL272 (purple) can be co-colonized and jointly generate a synergistic probiotic effect on the plant. The plant probiotic complex microbial inoculant not only can significantly promote cucumber growth, but also can help plants to resist salt in soil with a salt content of 0.3%.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to the development and application of a plant growth-promoting compound bacterial agent based on rhizosphere biofilm co-colonization. Background technique [0002] Composite microbial bacterial agent is a bacterial agent product composed of two or more microorganisms. The essence of the composite microbial agent is artificially designed synthetic microbial ecology, which is mainly aimed at specific purposes, using rational design of microbial combinations or ecosystems with new functions and behaviors. Compared with single microorganisms and natural microbial groups, synthetic microbial communities have the advantages of low complexity, high controllability, and good stability. The research on the application of synthetic microbial communities, that is, compound bacterial agents, in agriculture is still in its infancy. How to use existing ecological theories to rationally d...

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Problems solved by technology

[0003] After the microbial fertilizer is applied to the soil, on the one hand, it needs to face the problem of living environment pressure caused by abiotic factors such as soil physical and chemical properties, and on the other hand, it also faces problems such as competition with indigenous microorganisms for living space, which restricts the growth-promoting bacteria. The colonization ability of the rhizosphere affects the field effect stability of microbial fertilizers and limits the development of microbial fertilizers

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