Stenotrophomonas sp and application thereof

A technology of oligotrophomonas and microbial inoculants, applied to oligotrophs and their application fields, can solve the problem that the growth promoting effect is not obvious, the growth promoting effect is unstable, and the organophosphate-solubilizing bacteria have not yet achieved large-scale application. and commercial production to achieve the effect of promoting crop growth

Inactive Publication Date: 2017-02-22
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the inconspicuous growth-promoting effect and unstable growth-promoting effect of organophosphorus-solving bacteria in application, large-scale application and commercial production of organophosphorus-solving bacteria have not yet been realized.

Method used

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  • Stenotrophomonas sp and application thereof
  • Stenotrophomonas sp and application thereof
  • Stenotrophomonas sp and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the separation and identification of organophosphorous bacteria C1-13

[0024] 1. Isolation of organophosphate bacteria C1-13

[0025] The separation of organophosphorous bacteria C1-13 includes two steps of sampling, screening and purification, the specific method is as follows:

[0026] 1.1. Sampling

[0027] The samples were collected from the turf rhizosphere soil in the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin.

[0028] 1.2. Screening and purification

[0029] Weigh 1g soil sample, place in 9ml sterile water and shake at high speed to make soil bacteria suspension, apply the soil suspension of gradient dilution on organophosphorus solid medium (glucose 10g, (NH) 2 SO 4 0.5g, MgSO 4 ·7H 2 0 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 0 0.03g, MnSO 4 ·H 2 O 0.03g, CaCO 3 5g, 20g agar, 1L distilled water, pH 7.0-7.5. After melting and cooling the medium to 50°C, immediately add egg yolk liquid (egg yol...

Embodiment 2

[0039] Example 2, Determination of the Organic Phosphorus Ability of Stenotrophomonas C1-13

[0040] Stenotrophomonas C1-13 was cultured on a shaker in beef extract peptone liquid medium for 1-2 days, then pipetted 5 μL and inoculated on organic phosphorus solid medium, cultured at 30°C for 7 days, and measured the phosphorus-dissolving circle diameter (HD) and colony diameter (CD).

[0041] The result is as figure 1 As shown in B, Stenotrophomonas C1-13 formed a relatively obvious phosphorus-dissolving circle on the organic phosphorus solid medium plate, and the HD / CD value was 3.28. It shows that CGMCC NO.12835 has the ability to convert insoluble organic phosphorus in soil into easily absorbed available phosphorus, which can make it easier for plants to absorb phosphorus, thereby promoting plant growth.

Embodiment 3

[0042] Embodiment 3, determination of the ability of Stenotrophomonas C1-13 to secrete growth hormone indoleacetic acid (IAA)

[0043] Stenotrophomonas C1-13 was inoculated into King medium (peptone 20g, glycerol 15mL, K 2 HPO 4 1.5g, MgSO 4 ·7H 2 0 1.5g, tryptophan 0.1g, pH 7.2 (adjust pH with KOH)), 30°C, 200rpm shaker culture for 24h. Take 3mL bacterial liquid and centrifuge at 12000rpm for 15min. Take 1mL of the supernatant and add 2mL of Salkowski's reaction solution (12mg / L FeCl 3 , 7.9mol / L H 2 SO 4 , add distilled water to 1L), mix well, react in the dark for 30min, and measure the absorbance value on a UV spectrophotometer with a wavelength of 530nm. The sterile medium was treated the same as above and set to zero as a control. With concentrations of 0, 5, 10, 20, 30, 40, 50, 60 μg·mL -1 The IAA standard solution was used to make a standard curve in the same way. Three replicates were set up for each sample.

[0044]According to the standard curve and dete...

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Abstract

The invention provides a stenotrophomonas sp C1-13 strain, which is preserved with a preservation number of CGMCC NO.12835. The stenotrophomonas sp C1-13 strain can significantly promote the growth of cucumber seedlings. Pot experiments show that the stenotrophomonas sp C1-13, in comparison with blank control, can improve the fresh weight of the cucumber seedlings by 42.85%, dry weight by 34.97% and plant height by 27.00%.

Description

technical field [0001] The invention belongs to the technical field of screening and application of beneficial microorganisms, and in particular relates to a Stenotrophomonas and its application. Background technique [0002] Phosphorus is one of the main nutrients needed for plant growth, and phosphorus in soil mainly exists in the form of insoluble phosphate. In order to increase the yield of agricultural products, people use large amounts of phosphorus fertilizers for a long time. Phosphorus fertilizers are quickly fixed to form ineffective phosphorus after application and become unavailable, resulting in low crop uptake and a large accumulation of phosphorus in the soil. Improving the utilization rate of phosphorus in soil has always been one of the hot topics of agricultural science and technology workers. There are some microorganisms in the soil that can convert the phosphorus that is difficult for plants to absorb and utilize into a state that can be absorbed and u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/10C05F11/08A01N63/00A01P21/00C12R1/01
CPCA01N63/00C05F11/08C12N1/20C12P17/10C12N1/205C12R2001/01
Inventor 王敬敬黄志勇王欢徐松赵维
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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