A method for promoting the production of extracellular polysaccharides by liquid fermentation of Ganoderma lucidum

A technology of liquid fermentation and Ganoderma lucidum, applied in the field of microbial fermentation, can solve the problems of manpower consumption, long cycle, and small solid fermentation output, and achieve the effects of reducing equipment and production costs, facilitating industrial production, and increasing polysaccharide production

Active Publication Date: 2020-11-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the culture methods of Ganoderma lucidum are solid fermentation and submerged liquid fermentation. The traditional solid-state fermentation method can obtain the fruiting bodies of Ganoderma lucidum, and then can extract active substances such as polysaccharides. The production cost is low, but the output of solid fermentation is small, labor-intensive, and the cycle is long , can no longer meet people's ever-increasing needs; liquid submerged fermentation technology cultivates Ganoderma lucidum, which greatly increases the production of active substances such as mycelia and polysaccharides, and has become the main method of modern ganoderma fermentation. However, stirring and aeration in the liquid submerged fermentation process , Aseptic control and other operations increase the production cost of Ganoderma lucidum polysaccharides, making the prices of active substances such as Ganoderma lucidum and polysaccharides remain high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Static culture compared with shaker culture: add 150ml (liquid level about 2cm) culture medium into 500ml Erlenmeyer flask, inoculate seed culture medium with a wet weight of 0.5g per 100ml fermentation broth, and culture at 30°C for 6 days; The shaker culture under the conditions was used as the control, and the shaker speed was 150r·min -1 . At the end of fermentation, the bacterial biomass and exopolysaccharide content were measured. When cultured statically, the exopolysaccharide produced per gram of bacteria was 0.179g; the exopolysaccharide produced per gram of bacteria in the control group was 0.086g.

Embodiment 2

[0024] Fermentation of Ganoderma lucidum with different liquid levels: Add appropriate amount of medium into a 500ml Erlenmeyer flask to control the liquid level at 0.1, 0.5, 1, 2, and 5 cm respectively, and inoculate seed liquid with a wet weight of 0.5 g per 100 ml of fermentation liquid. Cultured at 30°C for 6 days. At the end of fermentation, the bacterial biomass and exopolysaccharide content were measured. When the height of the liquid level is 0.1cm, the exopolysaccharide produced by each gram of bacteria is 0.104g; when the height of the liquid level is 0.5cm, the extracellular polysaccharide produced by each gram of bacteria is 0.148g; At 1cm, the exopolysaccharide produced per gram of bacteria is 0.166g; when the liquid level is 2cm, the exopolysaccharide produced per gram of bacteria is 0.179g; The exopolysaccharide produced was 0.242g.

Embodiment 3

[0026] Culture medium and culture method are the same as embodiment 1. The height of the liquid level in the experimental group was 0.5cm, and it was placed on a shaking table at 30°C and 150r min at intervals of 6, 12, 24, and 48 hours respectively. -1 Cultured for 10 min, and the control group was cultured statically throughout. The fermentation time is 6 days. At the end of fermentation, the bacterial biomass and exopolysaccharide content were measured. When the interval time is 6h, the extracellular polysaccharide produced by each gram of bacteria can reach 0.127g; when the interval time is 12h, the extracellular polysaccharide produced by each gram of bacteria can reach 0.136g; The extracellular polysaccharide produced by one gram of bacteria can reach 0.145g; when the interval time is 48h, the extracellular polysaccharide produced per gram of bacteria can reach 0.157g; Up to 0.148g.

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Abstract

The invention discloses a method for producing exopolysaccharides by promoting fermentation of lucid ganoderma liquid, and belongs to the field of microorganism fermentation. According to the method, liquid shallow fermentation of lucid ganoderma is adopted, the liquid level height of a fermentation culture medium is controlled to be 0.1-5cm, and the yield of ganoderan can be increased. The method has a relatively short fermentation cycle in comparison with solid-state fermentation, does not strictly need stirring, ventilating and other devices in comparison with liquid-state fermentation, the equipment and production cost can be reduced, the polysaccharide yield of unit thallus can be remarkably increased, and 0.086g / g thallus is increased to 0.243g / g thallus, which is increased by 1.83 times, so that industrial production is benefited.

Description

technical field [0001] The invention relates to a method for promoting the production of extracellular polysaccharides by ganoderma lucidum liquid fermentation, which belongs to the field of microbial fermentation. Background technique [0002] Ganoderma lucidum is the most famous large basidiomycete in the world, which has high nutritional value and health care function. Ganoderma lucidum polysaccharide is one of the main active substances of Ganoderma lucidum, which is divided into Ganoderma lucidum intracellular polysaccharide in the fruiting body of Ganoderma lucidum and Ganoderma lucidum mycelium, and Ganoderma lucidum exopolysaccharide in the Ganoderma lucidum liquid medium. At present, the culture methods of Ganoderma lucidum are solid fermentation and submerged liquid fermentation. The traditional solid-state fermentation method can obtain the fruiting bodies of Ganoderma lucidum, and then can extract active substances such as polysaccharides. The production cost is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/645
CPCC12P19/04
Inventor 丁重阳王琼艾连中李进伟苑畅汪瑞石贵阳
Owner JIANGNAN UNIV
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