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Application of sphingomonas strain xp in degrading polyphenolic compounds in tobacco stems

A technology of polyphenolic compounds, sphingosine cells, applied in the field of microorganisms

Active Publication Date: 2014-05-07
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the content of polyphenolic compounds exceeds a certain range, the tobacco leaves have various quality defects in varying degrees, such as: green miscellaneous and irritating, monotonous aroma, insufficient aroma, etc., and cannot be directly used to produce cigarettes
More importantly, excessive polyphenol compounds are toxic to the human body and will affect the health of smokers

Method used

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  • Application of sphingomonas strain xp in degrading polyphenolic compounds in tobacco stems
  • Application of sphingomonas strain xp in degrading polyphenolic compounds in tobacco stems
  • Application of sphingomonas strain xp in degrading polyphenolic compounds in tobacco stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 : Determination of the ability of Sphingomonas xp to utilize chlorogenic acid for growth

[0036] 1) Preparation of seed culture medium: Resuscitate the strain xp with LB medium, then inoculate it into inorganic salt medium containing 0.5g / L chlorogenic acid, culture it on a shaker at 30°C for 24 hours (rotation speed 200rpm), and collect the bacteria by centrifugation , washed 3 times with inorganic salt medium, then resuspended the cells with inorganic salt medium, adjusted to OD 600 When the value is 3.0, the resulting suspension is the seed culture solution.

[0037] 2) The growth ability of Sphingomonas: Add the seed culture solution to the inorganic salt medium to obtain the initial OD 600 value of 0.15, then add 0.5g / L chlorogenic acid, take a sample at 48h, detect the turbidity of the bacterial liquid and the content of chlorogenic acid (the content of chlorogenic acid is determined by high performance liquid chromatography), the results are shown...

Embodiment 2

[0039] Example 2: Determination of the ability of Sphingomonas xp to grow using tobacco stem extract

[0040] 1) Preparation of tobacco stem extract: Weigh 1g of tobacco stem, crush it, add 20ml of 60% ethanol, extract in a water bath at 50°C for 50min, shake well every 10min, filter after extraction, and the filtrate is the tobacco stem extract.

[0041] 2) Preparation of seed culture medium: resuscitate the strain xp with LB medium, then inoculate it into inorganic salt medium containing 0.1 ml / L tobacco stem extract, culture it on a shaker at 30°C for 24 h (rotation speed 200rpm), and collect it by centrifugation Bacteria, washed with inorganic salt medium for 3 times, then resuspended bacteria with inorganic salt medium, adjusted to OD 600 When the value is 3.0, the resulting suspension is the seed culture solution.

[0042] 3) The growth ability of Sphingomonas: Add the seed culture solution to the inorganic salt medium to obtain the initial OD 600 value of 0.15, and ...

Embodiment 3

[0044] Example 3: After the Sphingomonas strain xp was cultured with chlorogenic acid as the sole carbon source, the determination of the degradation ability of polyphenols in tobacco stems and the influence on the taste of tobacco stems

[0045] 1) Preparation of seed culture solution: the preparation method is the same as in Example 1.

[0046] 2) Degradation ability of Sphingomonas xp: 50ml of seed culture solution was evenly sprayed on the surface of 500g of cut tobacco stems at 30°C and 60% humidity, and samples were taken at different times to detect the content of polyphenols in tobacco stems . For the control group, 50 ml of distilled water was used to spray evenly on the surface of 500 g of shredded tobacco stems. The tobacco stems used in the experiment were selected from Henan 2009 tobacco stems.

[0047] The polyphenol content was determined by Folin’s phenol method: take 1g of tobacco stems and put them into a centrifuge tube, add 20ml of 60% ethanol and extra...

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Abstract

The invention belongs to the technical field of microorganisms strains, and in particular relates to a sphingomonas strain xp, wherein the sphingomonas strain xp is classified and named as (i) Sphingomonas ( / i) sp (i). ( / i); the preservation number of the strain xp in China Center for Type Culture Collection is CCTCC No: M2011453. The sphingomonas strain xp is applied to degrading polyphenolic compounds in tobacco stems, and is good in degradation effect.

Description

[0001] technical field [0002] The invention belongs to the technical field of microorganisms, and in particular relates to a sphingomonas strain xp and its application in degrading polyphenol compounds in tobacco stems. Background technique [0003] Sphingomonas is widely distributed in the environment and has strong tolerance to harsh environments (for example, Sphingomonas has been found in extreme environments such as polar regions and strong radiation). Sphingomonas can degrade a wide range of substrates, such as the degradation of aromatic compounds such as dioxins, phenols, azo dyes, and heterogeneous biomass polymers. Because Sphingomonas has a very wide range of metabolic capabilities for aromatic compounds, and some species of this genus can synthesize valuable extracellular biopolymers, Sphingomonas has become a concern in recent years. and research hotspots. [0004] Tobacco stems are an important part of tobacco leaves. After being separated by threshing and r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B5/16C12R1/01
Inventor 马宇平周浩李怀奇郝辉许平唐鸿志
Owner CHINA TOBACCO HENAN IND
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