Screening, identification and biocontrol activity determination methods for acidophilic PGPR strains

Abstract

The invention discloses screening, identification and biocontrol activity determination methods for acidophilic PGPR strains. The screening method comprises the following steps of: 1, screening of theacidophilic PGPR strains: adjusting the pH value of a nutrient agar culture medium plate to 5.5 for screening of acidophilic bacteria, wherein rhizosphere soil sample suspension is not subjected to heat treatment at 80 DEG C; and 2, determination of the bacteriostatic activity of the strains under different pH conditions: (1) preparing fermentation liquor of test strains, namely inoculating activated strains into NB with different pH values in 1.1. The invention relates to the technical field of tobaccos. The screening, identification and biocontrol activity determination methods for the acidophilic PGPR strains are based on treatment of bacteria with bacteria, bacteriostatic activity, disease prevention and growth promotion effects and related biocontrol characteristics of the strains onralstonia solanacearum are studied under acidic and neutral conditions respectively, the strains are classified and identified by combining BIOLOG-GEN-III identification and 16SrDNA gene sequence homology analysis, and a foundation is laid for providing efficient microbial resources for improving the biocontrol effect of tobacco bacterial wilt in acidic soil and application of the efficient microbial resources.

Description

technical field [0001] The invention relates to the technical field of tobacco, in particular to a screening, identification and biocontrol activity method of an acidophilic PGPR strain. Background technique [0002] Tobacco bacterial wilt is a devastating soil-borne disease caused by R. solanaceae, which causes huge economic losses to tobacco production every year. There are many factors that affect the occurrence of tobacco bacterial wilt, among which soil environmental conditions are the most important Influencing factors include soil type, pH, trace element content, etc. In recent years, due to factors such as large-scale continuous cropping, environmental pollution, and application of acidic fertilizers, the acidification of tobacco planting soil in my country has become increasingly serious. [0003] Soil acidification is closely related to the occurrence of bacterial wilt. The pH value of the soil where bacterial wilt occurs is significantly lower than the normal soil ...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a screening, identification and biocontrol activity method of an acidophilic PGPR strain, which solves the problem that bacterial wilt in the acidified tobacco planting soil seriously restricts the yield and quality of tobacco

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