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Screening method of bacillus subtilis with sodium chloride tolerance

A technology of Bacillus subtilis and sodium chloride, which is applied in the field of microorganisms and can solve problems such as limiting the application of Bacillus subtilis

Inactive Publication Date: 2012-02-08
WIN WIN GROUP
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  • Claims
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Problems solved by technology

This limits the use of Bacillus subtilis in fertilizers

Method used

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  • Screening method of bacillus subtilis with sodium chloride tolerance

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Embodiment Construction

[0012] The present invention will be described in further detail below through specific examples, so as to help to understand the content of the present invention.

[0013] The source of Bacillus subtilis subsp.subutilis was purchased from China General Microorganism Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, with the number 1.0769.

[0014] Isolation basal medium used: nutrient agar medium

[0015] Peptone: 10 grams

[0016] Beef powder: 3.0 grams

[0017] Sodium chloride: 5.0 grams

[0018] Water: 1000ml

[0019] pH value: 7.3±0.1

[0020] Screening acclimation method:

[0021] (1) Preparation: Prepare the medium according to the standard of nutrient agar medium, together with the packaged petri dish, test tube medium, sterile water, glass scraper, pipette and other auxiliary equipment, after high temperature sterilization, it will be ready for use. Pour the sterilized nutrient agar medium onto a flat plate, place the tes...

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Abstract

The invention belongs to the field of microbes, and relates to a screening method of bacillus subtilis with sodium chloride tolerance. The method comprises the following steps of: (1) screening, wherein bacillus subtilis with the CGMCC (China General Microbiological Culture Collection Center) preservation number of 1.0769 is subjected to test tube slant culture and a petri dish slant streak culture by using nutrient agar as a culture medium to screen a single colony of typical bacillus subtilis, and the typical bacillus subtilis is transformed to a slant of a nutrient agar test tube for culturing; and (2) acclimation, wherein sodium chloride is added into the nutrient agar culture medium to prepare petri dishes with different concentrations of sodium chloride. According to the method, thescreening and acclimating cultivation are carried out by gradually increasing the concentration of sodium chloride, so that the bacillus subtilis capable of tolerating high-concentration sodium chloride can be screened. The bacillus subtilis capable of tolerating 12.5wt% sodium chloride can be prepared and can be used in a fertilizer.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a method for screening and domesticating Bacillus subtilis and the obtained Bacillus subtilis, which are applied in fertilizers to improve the tolerance of Bacillus subtilis to salt. Background technique [0002] Bacillus subtilis, Latin scientific name Bacillus subtilis, is a kind of Bacillus. A single cell is 0.7-0.8×2-3 microns, evenly colored. Uncapsulated, with periosteum flagella, able to move. Gram-positive bacteria, spores 0.6-0.9×1.0-1.5 microns, elliptical to columnar, located in the center of the bacterium or slightly biased, the bacterium does not expand after spore formation. The surface of the colony is rough and opaque, stained white or yellowish, and often forms wrinkles when growing in liquid medium. Aerobic bacteria. It is widely used in genetic research, and the synthesis pathway and regulation mechanism of purine nucleotides in this bacterium are relatively cle...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/20C12R1/125
Inventor 李宝成尹川冯作山解丽娟
Owner WIN WIN GROUP
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