Method of planting plant symbiotic mycorrhizal fungi

A technology of symbiotic mycorrhizae and production methods, which is applied in botany equipment and methods, plant cultivation, gardening, etc., can solve the problems of cumbersome operation process, unfavorable application, and high cost, and achieve simple operation, significant economic benefits, and low cost Effect

Inactive Publication Date: 2017-05-03
李兴全
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology makes it easier than previous methods that were expensive or had issues with producing them at larger scales. It can be used on different types of products without requiring complicated operations like those previously mentioned.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the culture conditions for producing beneficial microorganisms from plant-based sources without causing harmful side effects on humans' health (such as those caused through exposure).

Method used

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Examples

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Effect test

Embodiment 1

[0010] Cultivation of Lactobacillus russula

[0011] Collect the fruiting body of Lactobacillus rubrum in the field, put it into agar medium, and cultivate mycelium in the culture room; prepare mycorrhizal synthetic culture substrate, containing 15 parts by weight of sepiolite mineral powder and 45 parts of peat Soil and 15 parts of perlite are mixed; the above-mentioned cultured mycelium is fully mixed with a sterile mycorrhizal synthetic culture medium, and spread on a layer of soil, humus, and quartz sand for cultivation; Sterilized germinated seed buds with a length of 0.2-0.8 cm (the seeds adopt the conventional germination method) Masson pine seeds are directly sown in the above-mentioned culture container, and made to form a culture substrate with mycorrhizal mixed with mycelium Mix evenly, and after cultivation, plant seedlings with mycorrhizae are obtained, and about 90 days, the sprouts of Masson pine are taken and observed under a dissecting microscope to find vigor...

Embodiment 2

[0013] Cultivation of matsutake mushroom

[0014] Commercially available matsutake fruiting bodies are put into agar medium, and mycelium is cultivated in a culture chamber; the mycorrhizal synthetic culture substrate is prepared, which contains 25 parts by weight of bentonite mineral powder, 65 parts of peat soil, 30 parts of Perlite mixed composition; the above-mentioned cultivated mycelium is fully mixed with sterile mycorrhizal synthetic culture medium, and spread on a layer of soil, humus, and quartz sand mixture for cultivation; the sterilized Seed buds that have germinated and have a length of 0.2-0.8 cm (conventional germination method is used for seeds) Masson pine seeds are directly sown in the above-mentioned culture container, and mixed evenly with mycorrhizal synthetic culture medium mixed with mycelium, cultivated , to obtain the plant seedlings with mycorrhizae, take the masson pine sprouts in about 100 days and observe under the dissecting microscope to find a ...

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Abstract

The invention discloses a method of planting plant symbiotic mycorrhizal fungi, and the method comprises the steps of a culturing a mycelium in a nutrient agar to put a spore or a sporophore organization of a target mycorrhizal fungi in the nutrient agar; b preparing a mycorrhiza synthetic medium that contains, by parts of weight, 15-25 parts of ore power mixture, 45-65 parts of peat soil or humus soil, 15-30 parts of perlite or quartz sand; c mixing the mycelium and the aseptic mycorrhiza synthetic medium fully, and paving a layer of aseptic matrix for cultivation; d planting a germinated host plant seed after disinfection in the culture container directly, and mixing the seed with a mycorrhiza synthetic medium containing the mycelium evenly, and obtaining a plant sprout with mycorrhiza after cultivation; e transplanting the plant sprout with mycorrhiza to a suitable growth location so that the plant and the fungi can grow together. The beneficial effects are that the operation is simple; the efficiency is high; and the cost is low.

Description

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Claims

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Application Information

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Owner 李兴全
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