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Method for detecting antibacterial property of silkworm excrement product

An antibacterial and product technology, applied in microorganism-based methods, biochemical equipment and methods, and microorganism measurement/inspection, etc., can solve the problems of inability to implement the antibacterial performance and evaluation of silkworm sand products, and achieve low cost and speed. Fast, easy-to-use effects

Inactive Publication Date: 2016-09-07
SUZHOU VOCATIONAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, to carry out antibacterial testing on silkworm excrement products to judge their antibacterial performance and efficacy, there is currently no existing detection method that can be used, and ultimately it is impossible to implement the antibacterial performance evaluation of silkworm excrement products

Method used

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Embodiment Construction

[0018] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0019] Embodiments of the invention include:

[0020] A method for detecting the antibacterial performance of silkworm excrement products, the specific steps are as follows:

[0021] Step 1: Add 2 mL of lyophilized bacteria broth to the bacterial freeze-dried bacteria powder, and the strain is Staphylococcus aureus ATCC 6538 or Escherichia coli ATCC 11299 or Escherichia coli ATCC8739 or Escherichia coli ATCC29522; culture at 37°C ± 1°C for 18 hours, and then use an inoculation needle to draw a line on the slant of nutrient agar medium, culture at 37°C±1°C for 24 hours, and the slant can be stored at 2-8°C for 1 month as a slant-preserved bacteria;

[0022] Step 2: Pick a typical bacterial colon...

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Abstract

The invention discloses a method for detecting antibacterial property of a silkworm excrement product, comprising the following steps: 1, scribing on a slant of a nutrient agar medium for inoculating to obtain slant preserved bacteria; 2, picking one typical bacterial colony from an agar slant, and inoculating to nutrient meat soup to obtain a bacterial suspension; 3, drawing and transferring the bacterial suspension into a test tube holding PBS, mixing well, and diluting to obtain inoculation bacterial suspension; 4, making sampling bags; 5, adding sterile water to one sampling bag for pre-soaking to obtain a blank control, adding an antibacterial silkworm excrement product and a non-antibacterial silkworm excrement product respectively into other sampling bags, and adding sterile water for pre-soaking to obtain a sample to be detected and a control sample; 6, placing the sample to be detected, the control sample and the blank control respectively on an agar plate surface coated with the inoculation bacterial suspension, taking a small amount of the inoculation bacterial suspension, and dropwise adding to the three samples; 7, during culturing, recording and acquiring antibacterial property of the silkworm excrement product. In the above manner, the method is simple to perform, high in speed and low in usage cost.

Description

technical field [0001] The invention relates to the antibacterial performance of silkworm excrement products, in particular to a method for detecting the antibacterial performance of silkworm excrement products with simple operation, high speed and low use cost. Background technique [0002] Silkworm excrement, also known as silkworm arrow, is mostly produced in Jiangsu, Zhejiang, Sichuan and other places. It is the dry excrement of silkworm moth larvae. Sweet and warm in nature and flavor, it has the effects of drying dampness, expelling wind, transforming turbidity, promoting blood circulation and relieving pain. Folks use silkworm excrement as pillow filling, which has the effects of clearing the liver and eyesight, nourishing the eyes, nourishing the brain, treating high blood pressure, and tinnitus, especially for infants and young children. The silkworm sand pillow is made of high-quality silkworm sand to keep the temperature of the head constant. It can really cool ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/19C12R1/445
CPCC12Q1/18
Inventor 王毅婧董斌
Owner SUZHOU VOCATIONAL UNIV
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