Method for detecting the number of live spore in Bacillus subtilis powder of 5 hundred billion live spores/gram

Abstract

The invention discloses a method for detecting the number of live spore in Bacillus subtilis powder of 5 hundred billion live spores / gram, comprising the steps of: A, formulation of reagent and culture medium as well as preparation of plating medium; B, pretreatment of sample; C, dilution of sample; D, sample coating and culture: 0.1mL of each of the samples which are diluted to 10 is absorbed to 5 nutrient agar plates on each of which sample numbers, dates and dilutions are marked, a coating rod is used to coat the samples that are put in the plates, until all the samples are absorbed by the culture medium, after three plates are coated, the coating rod is used to coat back and forth on the blank nutrient agar plates 20 times; another coating rod is used to coat the samples that are in the rest two plates until all the samples are absorbed by the culture medium, afterwards, the coating rod is used to coat back and forth on the blank nutrient agar plates 20 times, after the coating is completed, the culture medium is put into a 37 DEG C constant-temperature incubator for 16hr-20hr; E, counting. The method is convenient and simple in operation, high in sensitivity and suitable for the detection of a large number of samples, counting the number of single colony on the plate and calculating the number of pore of Bacillus subtilis in the sample.

Description

Technical field [0001] The invention relates to the technical field of biological detection of the number of live spores of 500 billion live spores / gram of Bacillus subtilis original powder, and more specifically to a method for detecting the number of live spores of 500 billion live spores / gram of Bacillus subtilis original powder. Background technique [0002] Since the 1950s, antibiotics have played a major role in the development of livestock, poultry and aquaculture industries as disease treatment agents and animal growth promoters. However, as the negative effects of antibiotics are becoming more and more serious, the World Health Organization has banned the use of any antibacterial drugs in feed. Feed and aquaculture have been greatly impacted by adding antibiotics to prevent and promote animal growth. The development and promotion of use has become an important issue worldwide, among which probiotics have received widespread attention. [0003] Probiotics, also known as m...

AI Technical Summary

Problems solved by technology

However, for the high content of Bacillus subtilis (500 billion cfu / g), due to the high degree of cohesion and aggregation of spores, it is not easy to disperse, and the thermal stability and biological activity of different spores are not the same, which makes the detection of spores difficult. Therefore, how to accurately and quickly detect high-content Bacillus subtilis spores has become the key to the quality control of this project