Method for preparing bacteria liquid for Riemerella anatipestifer vaccine

Abstract

The invention discloses a method for preparing bacteria liquid for Riemerella anatipestifer vaccine, which comprises the propagation and identification of the first-level seed, and the propagation and fermentation of the second-level seed; the propagation and identification of the first-level seeds comprises the following steps of: inoculating freeze-dry basic microbial strain into the nutrient broth containing 10 percent of fetal bovine serum, and culturing the nutrient broth for 24h at the temperature of 37 DEG C; inoculating the obtained product onto the nutrient agar plate containing 10 percent of fetal bovine serum by streaking, then culturing the obtained product in the environment containing 5 to 10 percent of CO2 or candle jar for 24h to 48h at the temperature of 37 DEG C; after that, selecting several typical colonies, inoculating the typical colonies onto the nutrient agar slant containing 10 percent of fetal bovine serum, culturing the product in the environment containing 5 to 10 percent of CO2 or candle jar for 24h at the temperature of 37 DEG C, and obtaining first-level seeds; the propagation of the second-level seeds comprises the following steps of: inoculating the first-level seeds into the nutrient broth containing 10 percent of fetal bovine serum; and culturing the nutrient broth for 24h at the temperature of 37 DEG C; sampling the obtained product for pureness test; and if obtained product is tested to be qualified, keeping the product at the temperature of 2 to 8 DEG C, wherein the service life thereof is not more than five days, the culture medium used in the fermentation is the N-J synthetic medium containing one percent of lysed whole blood, the fermenting microbial strain is inoculated in an amount which is 2 percent based on that of the second-level seed liquid, the fermentation manner is liquid fermentation and culture in a culture tank, and the culture condition is continuously culturing 24h at the temperature of 37 DEG C.

Description

technical field [0001] The invention relates to the technical field of veterinary biological preparations, in particular to a method for producing bacterial liquid for R. anatipestifer vaccine. Background technique [0002] Duck infectious serositis is a contagious disease that occurs in domestic ducks, geese, turkeys and a variety of poultry. Syndrome, duck septicemia and new duck disease, the pathogen is Riemerella anatipestifer (Riemerella anatipestifer, RA). The disease presents as acute sepsis or chronic infection. Clinically, the main manifestations are cough, panting, diarrhea, increased eye and nose secretions, ataxia, and head and neck tremor. Perihepatitis, air sacculitis, meningitis, and caseous salpingitis. The disease can occur all year round, and it is serious in winter, mainly through contaminated feed, drinking water, droplets, etc.; 1-8 week-old ducks are highly sensitive, especially ducklings 2-3 week old have a higher incidence High, up to 90%; the mort...

AI Technical Summary

Problems solved by technology

Large-scale fermentation and production of Riemeria anatipestifer bacteria liquid is one of the foundations and keys for the production of R. anatipestifer inactivated vaccines. Fermentation process

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