Process for recovering enterovirus in sewage

An enterovirus and sewage technology, applied in the field of virus recovery in sewage, can solve the problems of low virus recovery rate, complicated operation, false positives, etc., and achieve the effects of broad application prospects, large amount of sewage treatment, and high virus recovery rate.

Inactive Publication Date: 2006-06-28
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all have their advantages, but there are many problems for the virus in concentrated urban sewage, mainly contain: the virus recovery rate is low (mostly less than 60%), and the effect is unstable; easily affected by water quality conditions, especially It is the limit of sewage turbidity; the amount of water sample that can be treated is too small (the amount of sewage treated by flocculation and sedimentation method is generally less than 1 liter), and has no practical application value; while recovering viruses, substances that are toxic to tissue cells in sewage are also removed Concentration, leading to false positive results; complicated operation and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for recycling enteroviruses in sewage, comprising the following steps:

[0016] (1) Add polyaluminum chloride to 100mL of sewage to make the aluminum ion content in the sewage reach 30mg / L, mix well, adjust the pH to 6.5, and adjust the temperature to 20°C;

[0017] (2) passing through the filter column, the filter material in the filter column is positively charged filter material;

[0018] (3) Repeat elution with 6 times the nutrient broth of 2 times the volume of the filter material for 3 times, then elute with 6 times the nutrient broth of 1 / 10 times the volume of the filter material, and collect the eluate;

[0019] (4) Adding 1 / 10 times of the molecular weight of the eluent volume to the eluent is polyethylene glycol with a molecular weight of 6000, and polyaluminum chloride is added so that the mass concentration is 0.004% (that is, 0.004 polyaluminum chloride is contained in 100ml solution). g), centrifuge at 6000r / min for 30min, discard the supernatan...

Embodiment 2

[0029] The manufacture of the positively charged filter material in the filter column consists of the following steps:

[0030] (1) Grinding and screening silica gel for chromatography to make the particle size 100 mesh, elutriating with ordinary distilled water, rinsing to remove fine particles, and autoclaving at 121°C.

[0031] (2) Add 10g AlCl to 1800mL deionized water 3 , add slowly several times to fully dissolve, add 80mL of 4mol / L NaCO 3 Dissolve, adjust the pH to 7.2, gel appears, remove the supernatant after the precipitate is obtained, wash twice with 0.14mol / L NaCl, then add 2000ml of 0.14mol / L NaCl, and autoclave.

[0032] (3) Add 2000mL of aluminum gel to 2750g of silica gel to soak and mix well, and dry at 45°C-60°C for later use.

Embodiment 3

[0034] In the making of the positively charged filter material in the filter column of embodiment 3, when step (1) sieves, also can pass 60 orders. The amount of deionized water in step (2) can also be selected 1900mL or 2000mL; AlCl 3 , the weight can also choose 11g or 12g or 13g or 14g; 4mol / LNaCO 3 The volume can also choose 100mL or 120mL or 150mL or 200mL.

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PUM

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Abstract

The invention discloses sewage enterovirus reclaiming method. It includes the following steps: adding poly-aluminum chloride or aluminum chloride in sewage; adjusting pH 5.5-8.5 and temperature 4-30 centigrade degree; passing through filtering column; eluting by six times nutrient broth and collecting the eluent; adding polyethylene glycol with 6000 molecular weight, poly-aluminum chloride or aluminum chloride into the eluent; centrifugation and removing the supernatant; adding normal saline to do suspensoid settlement and constant volume. The invention can make the virus recovery rate rise to over 80%. The invention has the advantages of cheap related tool material sources, high virus recovery rate, large sewage disposal quantity, and stable effect.

Description

technical field [0001] The invention relates to a method for recovering viruses in sewage, in particular to a method for recovering enteroviruses in sewage. Background technique [0002] Various pathogenic viruses existing in the environment have caused great harm to human health. At present, it has been confirmed that urban sewage, especially domestic sewage, contains a large number of pathogenic enteroviruses, including poliovirus, coxsackievirus, echovirus, rotavirus, hepatitis A virus and so on. The Lancet magazine also wrote that more than 100 enteroviruses can be detected in human feces and domestic sewage, and the content can be 5×10 5 More than pfu / L. These viruses use water as a medium, leading to outbreaks of infectious diseases. If the efficient and rapid detection and monitoring of pathogenic viruses in some environmental water bodies (such as river water, domestic sewage, and industrial wastewater) can be carried out, it will play a very positive role in isol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/02
Inventor 李君文王新为张小英金敏谌志强
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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