Bacillus megaterium strain, and preparation method and application thereof

The technology of bacillus and strain is applied in the field of bacillus strain and its preparation, which can solve the problems of soil structure change and excessive ions, and achieve the effects of adjusting soil pH, promoting aggregate structure and increasing soil organic matter content

Inactive Publication Date: 2019-12-10
BEIFANG UNIV OF NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, the existing problems in the prior art are: current saline-alkali land improvement measures are likely to cause changes in soil structure and introduce too many ions;

Method used

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  • Bacillus megaterium strain, and preparation method and application thereof
  • Bacillus megaterium strain, and preparation method and application thereof
  • Bacillus megaterium strain, and preparation method and application thereof

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preparation example Construction

[0026] Such as figure 1 As shown, the preparation method of the bacillus strain provided by the embodiments of the present invention comprises the following steps:

[0027] S101: Activation of strains: Cultivate the cryopreserved bacillus on NA solid medium to obtain activated seeds of the bacillus;

[0028] S102: Preparation of strain seed solution: activate the bacillus, take the single colony of the activated bacillus and streak it on the medium, then seal it and put it in the incubator for cultivation to obtain the activated strain, and transfer the activated strain to Cultivate in the culture medium to obtain the strain seed solution;

[0029] S103: Fermentation broth preparation: insert the cultured bacillus seed solution into the fermentation medium with an inoculation amount of 3%-8%, and the culture conditions are as follows: ventilation rate 0.7-2.3vvm, rotation speed 300-500rpm, temperature 30-36°C , the culture time is 48-60h, the bacterial suspension of bacillus...

Embodiment 1

[0035] The Bacillus megaterium strain of the embodiment of the present invention, the bacterial strain number is YM11, and it is preserved in the China Type Culture Collection Center located in Wuhan, and the preservation number is CCTCC M 2018430. Bacillus megaterium strain YM11 is a kind of bacillus, which has the ability to improve the salt-alkali tolerance of corn, In addition, it can produce substances such as organic acids, auxins and antibiotics, which can stimulate crop growth, adjust soil pH, promote soil aggregate structure, inhibit the growth of soil pathogenic bacteria and improve soil micro-ecological environment. The maximum salt tolerance of YM11 strain itself is 11 %, the maximum pH tolerance is 11.3, so that it can survive in a saline-alkali environment more stably, and the experimental verification shows that the YM11 strain can stably colonize the rhizosphere of crops to play a role.

[0036] The preparation method of the Bacillus megaterium strain of the emb...

Embodiment 2

[0046] The preparation method of Bacillus megaterium strain of the present invention comprises the following steps:

[0047] A, bacterial classification activation: the bacillus bacillus that cryopreservation is cultivated on the NA solid medium, obtains the activated seed of bacillus;

[0048] B. Preparation of strain seed solution: Activate the bacillus, take the single colony of the activated bacillus and streak it on the medium, then seal it and put it in an incubator for cultivation to obtain an activated strain, and transfer the activated strain to Cultivate in the culture medium to obtain the strain seed solution;

[0049] C. Preparation of fermentation broth: insert the cultured bacillus seed solution into the fermentation medium with an inoculum size of 8%, and the culture conditions are 2.3vvm of ventilation, 500rpm of rotating speed, 36°C of temperature, and 60h of cultivation time to obtain bacillus Bacterial suspension, centrifuge the bacterial suspension and col...

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Abstract

The invention belongs to the technical field of microbial agents, and discloses a bacillus megaterium strain, and a preparation method and application thereof. The collection number of the bacillus megaterium strain is CCTCC (China Center for Type Culture Collection) M2018430. The preparation method for the bacillus megaterium strain comprises the following steps of: culture activation: carrying out activated culture on the bacillus megaterium preserved at a low temperature on an NA (Nutrient Agar) solid culture medium to obtain the activation seed of the bacillus megaterium; and culture seedsolution preparation: inoculating the bacillus megaterium obtained after activation is conducted into an NA liquid culture medium, and carrying out rejuvenation culture. The bacillus megaterium disclosed by the invention has the functions of stimulating crops to grow, regulating soil acility and alkalinity, accelerating soils to form a granular structure, inhibiting the growth of soil pathogenic bacteria and improving the micro-ecological environment of soils. By the application of the Bacillus megaterium strain YM11, the micro-ecological environment of a maize rhizosphere can be improved, rhizosphere nutrient element compositions are improved, the micro-ecological environment of the maize rhizosphere is changed, soil microbial diversity is increased, soil physicochemical properties are improved, and the yield of maize on a saline-alkali soil is increased.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, in particular to a bacillus strain and its preparation method and application. Background technique [0002] At present, the closest existing technology: saline-alkali soil is a general designation of saline soil, alkaline soil and various salinized and alkalized soils. According to incomplete statistics from UNESCO and the International Food and Agriculture Organization, the saline-alkali land area in the world accounts for nearly 7.6% The land area causes serious losses to the world's food production every year, and seriously affects the ecological balance of the region. In my country, secondary salinized farmland accounts for 10% of the total cultivated land. Due to improper cultivation and management, my country's secondary salinization degree of intensification. my country's saline-alkali soil is mainly distributed in large agricultural regions such as North China, Northeas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/11
CPCC12N1/20A01N63/00C12R2001/11C12N1/205
Inventor 杨国平张琇王华笑刘环王静李敏杨晨希刘雅琴李壮胡子轩
Owner BEIFANG UNIV OF NATITIES
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