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Bacterium culture medium and preparation method therefor

A technology of bacterial culture medium and culture medium, applied in the direction of bacteria, etc., can solve the problems of cumbersome detection procedures, inability to directly observe and calculate the measurement results, etc., and achieve the effect of simple and convenient operation and reasonable process design

Inactive Publication Date: 2004-12-29
胡常英
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Problems solved by technology

In the above-mentioned background technology, there are the shortcomings of cumbersome detection procedures, inability to directly observe and calculate the measurement results

Method used

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Embodiment Construction

[0022] Embodiments of the present invention are further described below:

[0023] In this embodiment, the bacterial culture medium is based on nutrient agar medium (GB4789.28-1994), and water-retaining agent and dyeing agent are also added in the basal medium, wherein, nutrient agar: water: water-retaining agent: dyeing agent The weight ratio is 38:1000:3:0.01.

[0024] When preparing bacterial culture media, the following steps should be followed:

[0025] a, preparing nutrient agar medium;

[0026] b. After weighing the prepared nutrient agar medium: water: water retaining agent: dyeing agent according to the weight ratio of 38: 1000: 3: 0.01, place them separately;

[0027] c. Mix the weighed nutrient agar medium with water, place it for 10 minutes, and boil it over low heat through the asbestos wire mesh to completely dissolve;

[0028] d. After crushing the water-retaining agent into 100-mesh particles, add it to the nutrient agar medium prepared in the above step, and...

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Abstract

The present invention relates to bacteria culture medium, and is especially one kind of bacteria culture medium with nutritious agar culture medium as basic culture medium and its preparation process. The basic culture medium contains nutritious agar, water, water preserving agent and dye in the weight ratio of 36-40 to 1000 to 0.1-5 to 0.01-0.1 and is prepared through mixing in the weight proportion and sterilizing. The present invention has the features of convenient observation and detection, simple preparation process and convenient operation.

Description

technical field [0001] The invention relates to a bacterial culture medium and a preparation method thereof, in particular to a bacterial culture medium based on a nutrient agar medium and a preparation method thereof. Background technique [0002] At present, most of the bacterial culture medium in the prior art is based on agar, and some nutrients required for bacterial growth are added, and then the bacteria are cultivated, observed and measured in an aseptic laboratory according to strict aseptic operating procedures. In the above-mentioned background technology, there are disadvantages that the detection procedure is cumbersome, and the measurement results cannot be directly observed and calculated. Contents of the invention [0003] The object of the present invention is to provide a bacterial culture medium and a preparation method thereof which are easy to observe the condition of the colonies and avoid dehydration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 胡常英马清河马允莉付丽萍王洁孙淑琴
Owner 胡常英
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