Method for screening sodium chloride-resistant bacillus licheniformis

A technology of Bacillus licheniformis and sodium chloride, applied in the field of microorganisms, can solve problems such as limiting the application of Bacillus licheniformis

Inactive Publication Date: 2012-02-08
WIN WIN GROUP
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Problems solved by technology

This limits the use of Bacillus licheniformis in fertilizers

Method used

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  • Method for screening sodium chloride-resistant bacillus licheniformis

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Embodiment Construction

[0013] The present invention will be described in further detail below through specific examples, so as to help to understand the content of the present invention.

[0014] The source of Bacillus licheniformis was purchased from the China General Microorganism Culture Collection (CGMCC) of the Institute of Microbiology, Chinese Academy of Sciences, with the number 1.0518.

[0015] Isolation basal medium used: nutrient agar medium

[0016] Peptone: 10 grams

[0017] Beef powder: 3.0 grams

[0018] Sodium chloride: 5.0 grams

[0019] Water: 1000ml

[0020] pH value: 7.3±0.1

[0021] Screening acclimation method:

[0022] (1) Preparation: Prepare the medium according to the standard of nutrient agar medium, together with the packaged petri dish, test tube medium, sterile water, glass scraper, pipette and other auxiliary equipment, after high temperature sterilization, it will be ready for use. Pour the sterilized nutrient agar medium onto a flat plate, place the test tube c...

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Abstract

The invention belongs to the field of microorganisms, and relates to a method for screening sodium chloride-resistant bacillus licheniformis, which comprises the following steps: (1) screening: through taking the nutrient agar as an agar medium, the slant culture of a test tube is firstly carried out on the bacillus licheniformis which has the number of 1.0518 and is preserved in the China general microbiological culture collection center (CGMCC), then after the slant streak cultivation of a culture vessel is carried out, the single bacterial colony of the typical bacillus licheniformis is screened; the typical bacillus licheniformis is transferred to the inclined plane of the nutrient agar test tube to culture; and (2) domestication: the sodium chloride is added in the nutrient agar culture medium to prepare the culture vessels with different sodium chloride concentrations, the sodium chloride concentration is gradually improved for screening and domestication culture, and the bacillus licheniformis which can resist the sodium chloride with higher concentration is screened. The bacillus licheniformis which can resist the sodium chloride with the mass concentration of 12.5% is obtained, and can be used for fertilizer.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a method for screening and domesticating Bacillus licheniformis and the obtained Bacillus licheniformis, which are applied in fertilizers to improve the tolerance of the Bacillus licheniformis to salt. Background technique [0002] Bacillus licheniformis, Latin scientific name Bacillus licheniformis, is a kind of Bacillus. Bacillus licheniformis cells are rod-shaped and solitary in shape and arrangement, can produce anti-active substances, and have a unique mechanism of biological oxygen capture, which can inhibit the growth and reproduction of pathogenic bacteria. [0003] Bacillus licheniformis can inhibit the reproduction of pathogenic bacteria in the soil and the invasion of plant roots, reduce plant soil-borne diseases, and prevent the outbreak of various pests, so it is used in fertilizers. For example, CN101955903A discloses "a Bacillus licheniformis and its application", and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/10
Inventor 李宝成尹川冯作山梁玉伟解丽娟
Owner WIN WIN GROUP
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