Long-term preservation and activation method of efficient degumming strain of ramie

Abstract

The invention discloses a long-term preservation and activation method of an efficient degumming strain of ramie. The long-term preservation and activation method comprises the following steps: picking a purified classical single colony lawn, scribing in a test tube solid nutrient agar culture-medium, and culturing in a constant temperature incubator with the temperature of 35DEG C for 20 hours; sheathing by a large test tube, plugging a rubber hollow glass tube, and coating a junction of the large test tube and rubber with paraffin; after a vacuum pump vaccumizes, sintering the hollow glass tube by using an alcohol blast burner, sealing and storing. After the efficient degumming strain of the ramie stored for 10 years by adopting the method is subjected to activation treatment, the test live bacteria amount can reach 1.02*109cfu / ml or above; after activated state bacteria liquid is inoculated to the ramie, and is soaked and fermented for 10.5 hours, ramie fibers are completely dispersed, the mass is reduced by 27 percent or above, and efficient degumming of the ramie is realized. According to the long-term preservation and activation method disclosed by the invention, the stored strain is small in required space and is long in storage time; the survival period of the strain exceeds 10 years; by one-time inoculation, transformed generation is not needed over 10 years; the strain is easy to activate and stable in performance.

Description

technical field [0001] The invention relates to the fields of agricultural technology and biotechnology, in particular to a method for long-term storage and activation of ramie high-efficiency degumming bacterial strains. Background technique [0002] In the prior art, many enterprises use enzyme preparations to carry out ramie degumming operation. The process flow is: binding-caging-enzyme training-retting-washing-alkali boiling-coiling-rinsing-oiling-deoiling water- Shaking hemp-drying hemp; it uses a bio-chemical combined degumming process, which greatly reduces the production of acid-base wastewater and the cost of raw materials compared with the chemical degumming process using acid-base presoaking; the degumming of degumming strains Low enzyme activity or incomplete secreted enzyme system will make ramie unable to meet the requirements of textile production for ramie fiber after degumming by microorganisms, so it is particularly important for the preservation of degumm...

AI Technical Summary

Problems solved by technology

Due to the characteristics of microbial strains that are easy to mutate, long-term use and excessive number of transfers will lead to a decrease in their vitality; in order to keep the strains from decaying as much as possible, their original purity and excellent properties can be maintained to facilitate production For the application of scientific research and other aspects, it is necessary to preserve the strains; there are many methods for the preservation of strains, mainly including: transfer slope low temperature preservation method, liquid paraffin preservation method, glycerin culture preservation method, semi-solid puncture preservation method, sand tube preservation method, liquid nitrogen cryopreservation method, cryogenic glycerol freezing method and vacuum freeze-drying preservation method, etc. Although some methods can be stored for a long time, they require more equipment and complicated operations; although some methods are relatively simple, the storage time is short and frequent Transduction is prone to variation. Based on the above reasons, it is necessary to explore a method for long-term preservation, stable properties, simple operation, no need for more equipment, and long-term preservation of highly efficient degumming strains.