Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting number of viable bacillus

A detection method and technology for spores, applied in the field of microorganism detection, can solve the problems of not being able to truly reflect the number of viable bacteria in spore products, failing to detect viable bacteria, etc., and achieve the effect of high accuracy

Inactive Publication Date: 2010-03-03
SHANGHAI CHUANGBO ECOLOGICAL ENG
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing spore live bacteria detection method is directly diluted and plated for culture. There are many live bacteria that cannot be detected and cannot truly reflect the number of live bacteria in spore products.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Two identical samples were taken from the same batch of solid products of Bacillus licheniformis, one of which was prepared according to the conventional method stipulated in the Chinese agricultural industry standard NY / T 1461-2007, for the preparation of the detection medium, the preparation of the plate, and the sampling of the sample , weighing, diluting, mixing plates, cultivating and calculating to obtain the effective number of viable bacteria of the sample, and the results are listed in Table 1.

[0016] Another sample uses the method provided by the invention to carry out the detection of effective spore quantity, and concrete steps are as follows:

[0017] The first step: take the spore sample according to the conventional method;

[0018] Second step: the above-mentioned spore sample is added in the triangular flask or test tube that nutrient broth germination agent is housed with weight ratio 1: 8, contains 0.001wt% manganese sulfate, 0.01wt% magnesium sulfa...

Embodiment 2

[0023] Two identical samples were taken from the same batch of solid products of Bacillus subtilis, one of which was prepared according to the conventional method stipulated in the Chinese agricultural industry standard NY / T 1461-2007, for the preparation of the detection medium, the preparation of the plate, and the sampling of the sample , weighing, diluting, mixing plates, cultivating and calculating to obtain the effective number of viable bacteria of the sample, and the results are listed in Table 2.

[0024] Another sample uses the method provided by the invention to carry out the detection of effective spore quantity, and concrete steps are as follows:

[0025] The first step: take the spore sample according to the conventional method;

[0026] Second step: the above-mentioned bacterium sample is added in the triangular flask or test tube that nutrient broth germination agent is housed with weight ratio 1: 10, contains 0.1wt% manganese sulfate, 1wt% magnesium sulfate an...

Embodiment 3

[0031] Two identical samples were taken from the same batch of solid products of Bacillus coagulans, one of which was prepared according to the conventional method stipulated in the Chinese agricultural industry standard NY / T 1461-2007, for the preparation of the detection medium, the production of the plate, and the sampling of the sample , weighing, diluting, mixing plates, cultivating and calculating to obtain the effective number of viable bacteria of the sample, and the results are listed in Table 3.

[0032] Another sample uses the method provided by the invention to carry out the detection of effective spore quantity, and concrete steps are as follows:

[0033] The first step: take the spore sample according to the conventional method;

[0034] Second step: the above-mentioned bacterium sample is added in the triangular flask or test tube that nutrient broth germination agent is housed with weight ratio 1: 9, contains 0.05wt% manganese sulfate, 0.05wt% magnesium sulfate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting the number of viable bacilli, which is characterized by comprising the following steps: weighing a bacillus sample according to a conventional method; adding the bacillus sample to a triangular flask or a test tube holding a nutrient broth germinant, wherein the broth germinant contains manganous sulfate, magnesium sulfate and ferrous sulfate; puttingthe triangular flask or the test tube in a constant-temperature water bath the temperature of which is 80 DEG C to carry out high-temperature treatment for 10-15 min; cooling with constant-temperaturerunning water; vibrating on a swing bed for 30 min, and then obtaining a bacillus culture solution; diluting the bacillus culture solution for 10 times with 0.5-1wt% of sodium chloride diluent; inoculating in a nutrient agar culture medium which is melted in advance and cooled to 50 DEG C, and after solidification, putting in a constant temperature cabinet the temperature of which is 37 DEG C tocarry out inverted culture for 48 hours, and then seeing that visible colonies grow out on a flat plate; and calculating according to the conventional method to obtain the number of the viable bacilliin the sample. The invention has the advantage of high accuracy.

Description

technical field [0001] The invention relates to a method for detecting the number of viable spores, belonging to the technical field of microbial detection. Background technique [0002] Probiotics, also known as probiotics or live bacterial preparations, are a type of active microbial additives. On the one hand, after being eaten by livestock and poultry, probiotics can develop, reproduce and play a beneficial role in the digestive tract, especially in the intestinal tract, which can promote the growth and development of livestock and poultry, improve feed utilization, reduce the mortality of young livestock and poultry, and prevent digestive tract diseases and improve the body's immunity; on the other hand, compared with the problems of drug residues and drug-resistant strains caused by the use of antibiotics, probiotics, as a new type of green additive, have no residues, no pollution, and no drug resistance. With its unique advantages, prebiotics have become promising ne...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12R1/07C12R1/10C12R1/125
Inventor 江瀚张玉辉
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com