103 results about "Bacilli class" patented technology

The invention relates to sulfur oxide sulfur bacilli and biological eliminating method of chromium in tanning mud. Bacterial strain is named as TS6, preserve mark is CGMCCNO.0759. The method of eliminate chromium in tanning mud by above mentioned sulfur oxide sulfur bacilli is adding 1-3 g / L sulfur into tanning mud in biology reactor, inocualting 10-15% (v / v, the same below) TS6 bacteria strain, stirring, ventilating and subsiding; that 10-20% of subsiding mud backflow to reactor; dehydrating suprplus subsiding mud; adding basic material into liquid part to turn Cr3+ into Cr(OH)3 and subside, sediment is dissolved by sulfuric acid, then there is the tannage stage; solid part is neutralized to agriculture. The elimianting rate of Cr in mud is 95%-100%, reserving rate of nutrient in mud is above 80%.

The invention relates to the field of medical image processing and mode recognition, in particular to a tubercle bacillus target recognizing and counting algorithm based on diverse characteristics. The algorithm comprises the following steps of: image preprocessing: carrying out image reinforcement and constructing median filter and Gaussian filter on a tubercle bacillus microimage; color image partition: carrying out fixed threshold partition based on HSV (Hue-Saturation-Value) color space on a preprocessed image and then carrying out adaptive threshold partition which is based on CIE L*a*b* color space and keeps a geometric shape of a target; communication block morphological analysis and target recognition: carrying out communication block analysis on the partitioned image; and tubercle bacillus target counting: estimating the quantity of tubercle bacillus targets in the image by utilizing a histogram statistics and multistrategy calculation method. The invention can effectively extract the bacillus targets in the tubercle bacillus microimage subjected to acid-fast stain from background and impurities and carry out accurate counting, thereby realizing the automation and the intellectualization of the detection of tubercle bacilli.

In view of the paucity of human material available to study the immunological events occurring after inhalation of virulent bacilli, but prior to development of clinical TB, the present invention is based in part on studies of aerosol infected rabbits. The present inventors reasoned that by 3-5 weeks post-infection, the sera from infected rabbits would contain antibodies to the antigens being expressed by the in vivo bacteria.

The invention supplies a method to produce organic fertilizer. The zymophyte agent is made up from brood cell bacilli, lactobacillus, photosynthetic bacteria, azotobacteria, yeast, filamentous fungus, and actinomycete. The expanding culture formula is 40% bean pulp, 30% wheat bran, 25% rice hull powder, 1.5% dextrose, 1% ZnSO4, 1% B, 1% MgSO4, and 0.5% peptone. The fermenting stockpile includes solid swine waste, 25% rice straw, 10% weathered coal, and 5% calcium superphosphate. The process steps includes: filtering swine waste, adding rice straw and weathered coal, adjusting carbon and nitrogen and water ratio, whisking to equal, inoculating microorganism fungus agent, high temperature fermenting, after being aged, drying, filtering, checking and packaging. The invention has the advantages of easy to operate, low cost, and less foul gas, etc.

The invention discloses a low-temperature-resistant lactobacillus strain which is lactobacillus plantarum CCZZ1, and an application thereof. The lactobacillus strain provided by the invention is collected at China General Microbiological Culture Collection Center with an address of Institute of Microbiology, Chinese Academy of Sciences, No.3, Court 1, West Beichen Road, Chaoyang District, Beijing. The strain has a collection number of CGMCC NO. 6078. A collection date is May 3rd, 2012. The lactobacillus strain provided by the invention is a Gram stain positive bacilli. The lactobacillus strain is glucose-homofermentative, and is acid-resisting. The strain has high growth speed and wide reproductive temperature range. With the strain, under a low-temperature or normal-temperature environment, silage fermentation quality can be substantially improved.

The invention discloses bacillus aryabhattai, microbial inoculum thereof, a preparation method and application. The bacillus aryabhattai BC104 is beta-cypermethrin degrading bacteria, and the preservation unit is the China General Microbiological Culture Collection center (CGMCC), the preservation date is 15th, Dec, 2015, and the preservation number is CGMCC No.11664; the bacillus aryabhattai belongs to mycomonera, phylum firmicutes, bacillus class, bacilli bacteria, bacillaceae and bacillus. The microbial inoculum with the bacillus aryabhattai as an active ingredient is the microbial inoculum obtained by preforming activation culture and fermentation culture on the bacillus aryabhattai BC104. The preparation method comprises the steps of activation culture and fermentation culture; the application of the microbial inoculum is to prepare a medicine for degrading beta-cypermethrin. The strain disclosed by the invention has the advantages of simple preparation process of the microbial inoculum, low cost, convenience in use and good application prospect.

The present invention provides monoclonal antibodies which are highly specific for Bacillus spores. Also provided are peptides derived from those monoclonal antibodies. Both the antibodies and peptides are highly specific and can discriminate between spores of potentially lethal organisms such as Bacillus anthracis and other harmless but closely related bacilli and provide a very powerful tool in the construction of detection instruments as counter measures.

A micro-bubble generating shower includes a shower body, which has defined therein a passage, through which tap water flows. A shower head is integrally provided in the upper portion of the shower body, and has a receptacle, which is connected to the passage. A bubble nozzle is coupled to the shower head, and generates micro-bubbles by converting dissolved oxygen contained in the tap water into ultra-micro-bubbles having a size on the micrometer scale. A discharge nozzle discharges the tap water that contains the micro-bubbles, which are generated by the bubble nozzle, under a predetermined pressure. The shower enhances the effects of purifying and sterilizing the tap water using the micro-bubbles, thereby eliminating skin bacilli.

The present invention concerns spore display methods. More specifically, the invention concerns display of heterologous molecules, such as peptides and polypeptides, on spores of bacilli, such as, for example, Bacillus thuringiensis (Bt) or Bacillus cereus (BC), using externally exposed spore coat proteins or fragments or variants thereof.

The invention discloses a bacillus amyloliquefaciens capable of degrading benzene compounds, wherein the 16S rDNA of the bacillus amyloliquefaciens has a sequence of SEQ ID No.1, the strain is pale orange red, round, smooth and humid with neat edges in a solid medium, and the diameter thereof of the strain is between 1 and 2 millimeters; the form of the bacillus amyloliquefaciens is a thicker bacilli and is about 10 micrometers in length; spores are formed and are oval; and the bacillus amyloliquefaciens is aerobic, gram-positive and contact oxidase negative, can use a BTEX mixture or a single substance as a unique carbon source, and can degrade the BTEX mixture efficiently.

The invention provides a method for degumming mulberry fiber with bacteria complex flora. The method uses mulberry as a culture medium for a carbon source to filter the bacteria complex flora with good degumming effect to the mulberry from sludge of a methane tank, substrate sludge of a pool, wood, banana land and other samples, has dried mulberry directly immersed into degumming liquid, and inoculates the cultured degumming complex bacterial flora into the degumming liquid for degumming, so that loose mulberry fiber can be obtained via water rinsing and drying. The method has the advantages of mild process condition and simple operation, need not use strong acid or strong alkali, has the manufacturing cost greatly reduced compared with a chemical degumming method, has lower environmental pollution, less cull in the prepared mulberry fiber and white and loose fiber.

A method of treating irritable bowel syndrome using a probiotic composition including the bacilli (1) Bacillus subtilis, (2) Bacillus coagulans, and (3) Enterococcus faecium. The composition may further include a carrier medium, such as fructo-oligo-saccharides (FOS), as incorporated in a dose form such as a pill, capsule, powder or sachet. The compositions of the invention may be usefully employed as health or nutritional supplements, food additives, or therapeutic agents for combating a wide variety of physiological disorders.

The invention relates to the technical field of bamboo artware processing, and discloses a method for mould and moth preventing for a bamboo product. The method comprises the following steps that firstly, newly-cut bamboo is wrapped by sludge in a pond, the sludge contains abundant nutrients suitable for growth of parasites, meanwhile, the viscous force is very large, the sludge has very strong adsorption force, disease pests and mould hidden in the bamboo are wrapped and adsorbed, and substances, causing insect damage and mould, in a bamboo body are transferred out; secondly, fumigated bamboo is placed in alums water to be immersed, alums has a very good restraining function on various gram negative and positive cocci and bacilli, Chinese herbal medicine liquid is used for fumigation after immersing, and it can be guaranteed that the bamboo product is not damaged by mould or moths for a long term through essential oil generated by the Chinese herbal medicine liquid; and thirdly, waterproof varnish is applied to the processed bamboo product, and the longer service life can be kept.

The invention relates to a process for preparing a viable bacillus subtilis preparation. According to the process, after bacillus subtilis undergoes first-stage germ amplification culture and second-stage germ amplification culture, the bacillus subtilis is inoculated to a fermentation culture medium for culture, and the number and conversion ratio of bacilli is increased through feeding a supplement carbon source during fermentation, so that the unit yield is increased remarkably, and the unit cost is reduced; at the end of fermentation, fermentation liquor is concentrated with a vacuum film concentrator, so that soluble synergistic substances, growth promoting substances and bacteriostatic active substances can be effectively recovered from the fermentation liquor; the concentrated fermentation liquor is subjected to drying and granulating by multi-level low-temperature spray-fluidized drying equipment, granules are subjected to sorting, fine powder gathered by a cyclone separator returns and is granulated again, and then, a bacillus subtilis product, of which the CFU of powder per gram is not less than 2.0*10<11>, is obtained; exhaust gas is treated and is discharged after reaching standard. The process has the advantages that effective substances in the fermentation liquor can be reserved to the maximum, the quality of the product is high, the equipment utilization ratio is high, the retention of biological activity is relatively good, waste gas and waste water are discharged after reaching standard, and large-scale industrial production can be achieved.

The invention provides three biocontrol bacilli namely bacillus methylotrophicus AR3, bacillus subtilis AR4 and bacillus amyloliquefaciens AR10 and application thereof to preparation of a crop ralstonia solanacearum resistant bacteria agent. According to the invention, three biocontrol bacilli namely bacillus methylotrophicus AR3, bacillus subtilis AR4 and bacillus amyloliquefaciens AR10 are obtained through soil sample taking, screening, analysis and cultivation in a healthy tobacco field in the Xuan'en county, results of plenty of field experiments prove that when being used separately or used in a mixed manner, the three biocontrol bacilli have the good effect of preventing and controlling crop ralstonia solanacearum, are highly targeted and low in pollution, and can be stored for a long term and conveniently used in the field after being prepared into the bacteria agent, so that the achievement of the bacilli in the field of biocontrol bacterium research is enriched and guiding significance on later related research work is realized.

The invention relates to LH microbes and their preparation method and use. The LH microbes are novel microbes which can effective treat sewage and reduce a discharge amount of sludge. The invention provides the preparation method and the use of the LH microbes. The LH microbes are combined multi-bacterium microbes. The combined multi-bacterium microbes comprise bacillus natto, bifidobacteria, bacilli, actinomycetes, photosynthetic bacteria, acetic acid bacteria, saccharomycetes, lactic acid bacteria and nitrifying bacteria.

There are described a simple examination method of infection with Helicobacter pylori possibly presenting in a gastric mucosa, and a device therefor. The examination is conducted by collecting gas in gastric cavity, and measuring mainly ammonia and additionally organic amines which are generated due to activities of the bacilli. The measurement is carried out by leading the gas in gastric cavity into oral cavity by vomiting-reflex, and sucking the gas by a metering suction pump through a gas detection tube to read-out a length of color-changed area in the gas detection tube.

The invention provides a method for preparing cellulase by bacilli. The method includes the steps of using bacillus as a producing strain, subjecting the producing strain to slant culture, shaking culture and fermenting culture to obtain the cellulase. Fermenting culture medium comprises, by weight percent, 0.5-2% of starch, 1-5% of soybean cake meal, 8-12% of soybean cake meal hydrolysate, 0.3-0.8% of bran, 1-2% of inorganic salt, and 0-0.3% of foaming agent. The cellulase prepared by the method has high activity in neutral pH and alkali conditions and is better than acidic cellulase in terms of water abrasion enzyme application. Starch material is used as a carbon source by the method, and accordingly the cellulase is better than that of the fiber material in terms of transfer, feeding, sterilization and the like. The soybean cake meal hydrolysate is used as a soluble nitrogen source, and accordingly the cellulase is low in cost and convenient to prepare and use. The cellulase prepared by the method is low in cost, high in activity, wide in pH application range, high in stability and short in production cycle.

The invention belongs to the field of biochemical engineering and processes, relates to a method for adsorbing metal ions by a biological adsorbent and in particular relates to an ochrobactrum intermedium extracellular polysaccharide and application of the ochrobactrum intermedium extracellular polysaccharide to the aspect of soil improvement. The method comprises the following steps: step (1): obtaining ochrobactrum intermedium capable of producing an extracellular polysaccharide; step (2): obtaining the ochrobactrum intermedium extracellular polysaccharide; step (3): adsorbing the metal ions through the ochrobactrum intermedium extracellular polysaccharide; step (4): desorbing the metal ions through the ochrobactrum intermedium extracellular polysaccharide.

The invention provides a method for detecting the number of viable bacilli, which is characterized by comprising the following steps: weighing a bacillus sample according to a conventional method; adding the bacillus sample to a triangular flask or a test tube holding a nutrient broth germinant, wherein the broth germinant contains manganous sulfate, magnesium sulfate and ferrous sulfate; puttingthe triangular flask or the test tube in a constant-temperature water bath the temperature of which is 80 DEG C to carry out high-temperature treatment for 10-15 min; cooling with constant-temperaturerunning water; vibrating on a swing bed for 30 min, and then obtaining a bacillus culture solution; diluting the bacillus culture solution for 10 times with 0.5-1wt% of sodium chloride diluent; inoculating in a nutrient agar culture medium which is melted in advance and cooled to 50 DEG C, and after solidification, putting in a constant temperature cabinet the temperature of which is 37 DEG C tocarry out inverted culture for 48 hours, and then seeing that visible colonies grow out on a flat plate; and calculating according to the conventional method to obtain the number of the viable bacilliin the sample. The invention has the advantage of high accuracy.

The invention aims to provide a new-generation brucellosis antibody competition enzyme-linked immunosorbent adsorption test detection kit which meets animal epidemic prevention requirements in China, is sensitive and specific and can accurately diagnose brucellosis. In the invention, the lipopolysaccharide of smooth type brucella is used as an antigen-coated enzyme-labeled plate, inactivated bacilli liquid is used for immunizing or artificially infecting a healthy cattle to prepare positive control sera and standard weak positive sera, healthy non-immune cattle sera are used as negative control sera, a novel monoclonal antibody is used as a competition antibody, the protein AG enzyme-labeled substance of an anti-Fc receptor is used as an assistant antibody, serum diluent, a washing solution, a substrate solution A, a substrate solution B, H2O2 and a stop solution are prepared, and all raw materials are assembled to form the kit. The invention is a competition ELISA kit which is sensitive and specific and can accurately diagnose brucellosis. The invention meets animal epidemic prevention requirements in China, and carries out last diagnosis on the brucellosis by means of a laboratory diagnosis technique.

One mycobacterium tuberculosis antigen detective method in tuberculosis bacilli solution, including the following steps: (a) Choice and prepare TB-specific antigen that can be detect by the antigen-antibody binding reactions in various body fluids; (b) Prepare of confrontation and strains of TB-specific antigen monoclonal antibody; (C) Prepare TB detection reagent kit of specific antigen secreted; (d) Use the above-mentioned antibody and antigen detection kit for tuberculosis. Take these steps and reach high rate of the test and correct testing purposes.

The invention relates to a series of nano-particle water purification materials, which take quartz sand or zeolite particle as a substrate, nanometer aluminum (iron) oxide or nanometer aluminum (iron) oxidized hydrogen oxide as a modified filtration coating, and can effectively and completely remove bacilli and virus from wastewater and sewage. The invention also relates to a preparation method of the nano-particle water purification materials. In the preparation method, acidic solution with 40 to100 meshes of quartz sand or 60 to120 meshes of clinoptilolite is poured into secondary deionized water, uniformly mixed and heated, nano-aluminum powder, aluminum nitride powder or iron powder is poured into the acidic solution and ultrasonically mixed and ethanol solution of ester is then poured into the mixed acidic solution, ultrasonically mixed again, filtrated and dried so as to gain nano-fibriform AlO(OH) or FeOOH which is the particle water purification materials of filtration coating which are further roasted so as to gain Gamma-Al2O3 or Fe2O3 which is the particle water purification materials of filtration coating. The nano-particle water purification materials of the invention have the advantages of strong removal ability, high cleanliness, great saturated adsorption capacity, etc.

This invention relates to a preparation method of using proteus mirabilis to prepare biofloculation agent. The craft as follows: (1) subcuitivate Proteus mirabilis to slant culture group, preserve at freezer of 2 to 6 degC;(2) inoculate conservative strain to seed culture group, then prepare seed liquid in rocker incubator at 25 to 30 degC; (3) by proportion of 1 to 2% inoculate seed liquid to sixpenny nutrient medium, then embed it in the rocker, at 25 to 30degC, 120 to 140r / min, 1 to 2 days to obtain strain nutrient medium fermentation broth, take centrifugalization 20 to 40 min by 4000 to 6000r / min centrifuge, remove sediment, the supernatant liquid is liquid biofloculation agent, measure flocculation ratio; (4) abstract and purify the liquid biofloculation to gain product. This invention has low cost, short production cycle, flocculation ratio can reach 92.88 to 93.13%, and has no secondary pollution, possess good generalization value.

The present invention provides an organic fertilizer and its method of preparation. The fertilizer is an okra based organic fertilizer comprises sterilized mature okra fruits, a colloidal fluid of bacilli, 10-15 fractions of chicken droppings, 20-25 fractions of soybean residuals, 10-15 fractions of plant ashes and 45-60 fractions of water. The fertilizer can condition the soil, increase the activity of microorganisms in the soil and facilitate to increase the biomass in an individual vegetable by more than 10%; it can contain the growth of the vegetable germs and effectively control various pathogenic bacteria harmful to flowers to significantly improve the vegetable quality with the use of pesticide to less than 90%. This organic fertilizer is applicable to plantation of vegetables or fruits, cereal crops and flowers with a broad application.

An object of the invention is to provide a bacterial growth inhibitor or bacteriostatic agent against thermo-resistant and acid-resistant bacteria (TAB: thermo acidophillic bacilli), such as bacteria belonging to the genus Alicyclobacillus, in particular, guaiacol-producible Alicyclobacillus acidoterrestris, Alicyclobacillus acidiphilus, and Alicyclobacillus herbarium, the bacterial growth inhibitor or bacteriostatic agent being derived from naturally occurring substances and facile in procurement, preparation, and handling. The invention provides a bacterial growth inhibitor or bacteriostatic agent against bacteria belonging to the genus Alicyclobacillus, which contains acerola fruit juice as an active ingredient.

The invention relates to a method to test pathogenicity vibrio parahaemolyticus in aquatic product that the feature is including the following steps: designing, filtering, and manufacturing the specificity primer of the testing strain, expanding PCR and testing, distilling DNA, PCR expanding of the testing strain specificity gene section, agarose gel electrophoresis of the PCR product and testing the specificity section; MPN method testing and calculating the testing result. The invention discloses a method to rapidly testing bacilli in testing sample.

The invention discloses a crystal pear biological antistaling agent based on mixed application of cryptococcus laurentii and antagonistic bacillus, a preparation method and application thereof. 12 strains of antagonistic bacilli are respectively employed for mixed application with the cryptococcus laurentii, they all can improve the postharvest biocontrol effect of crystal pears, and four strains can reach the best effect. Finally, the application concentration and ratio of the antagonistic bacillus and cryptococcus laurentii for a best mixed application effect are determined. Every 1ml of a control agent contains 106-109 antagonistic bacilli and 106-109 cryptococcus laurentii, and the best control effect can be ensured when the bacterial number ratio is 1:1. The cryptococcus laurentii and antagonistic bacillus involved in the biological antistaling agent both have the characteristics of safety and high efficiency, and have the advantages of environmental friendliness, and outstanding biocontrol effects, etc. the mixed bacteria antistaling agent provided by the invention can further reduce the application of chemical bactericides, and has important significance to food safety, environmental protection, agricultural income increase and synergy, and sustainable social development, etc.

The invention relates to a compound biological bacterial fertilizer. The compound biological bacterial fertilizer is prepared from the following raw materials: Bdellovibrio BDS01, Bdellovibrio BDS02, Nitrosomas, denitrifying bacteria, Streptococcus thermophilus, Saccharomyces cerevisiae, Lactobacillus bulgaricus, Leuconostoc, Pediococcus, Bacillus subtilis BSD-2, Actinomyces S317, Trichoderma T36, Bacillus spp B135, Saccharomyces E7, lactic acid bacteria L2, Bacillus coagulans, aerobic spore-bearing bacilli, anaerobic lactic acid bacillus, Rhodopseudomonas palustris, Pseudoalteromonas, asparagus bacteria, Firmicutes, Planctomycetes, cyanobacteria, Actinomyces, Bacillus natto, live bacteria of Clostridium butyricum, photosynthetic bacteria and butyric acid bacteria. The compound biological bacterial fertilizer provided by the invention is applicable as a plantation fertilizer and a biological feed and can protect the environment and human health.

The invention belongs to the technical field of biological engineering, in particular relates to a quantitative analysis method of lactic acid bacteria and bacilli in a yeast microbial community and aims to provide a new choice for analysis of the lactic acid bacteria and the bacilli in a yeast. According to the technical scheme, the quantitative analysis method of the lactic acid bacteria and the bacilli in the yeast microbial community comprises the following steps: a, preparation of a standard curve; b, extraction of a genome DNA (Deoxyribonucleic Acid); c, amplification; d, quantitative analysis. The method can be used for analyzing the variation trend of the lactic acid bacteria and the bacilli in pit mud.