Method for preparing biological flocculant using strange deformation bacilli

A technology of Proteus mirabilis and biological flocculant is applied in the field of preparation of biological flocculants, and achieves the effects of low cost, reduced cost and short fermentation period

Inactive Publication Date: 2007-11-14
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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However, so far, there have been no reports on the preparati

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  • Method for preparing biological flocculant using strange deformation bacilli
  • Method for preparing biological flocculant using strange deformation bacilli
  • Method for preparing biological flocculant using strange deformation bacilli

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Embodiment 1

[0024] This implementation mode is carried out according to the following steps:

[0025] A. Inclined preservation and transfer of strains

[0026] The composition (g / L) of the slant culture medium for the preserved strains is: beef extract 5, agar 20, peptone 12, NaCl 6, pH 7.2, sterilized (121° C., 30 min). In an aseptic operating environment, the strains of Proteus mirabilis were placed on the slant medium, cultured in a constant temperature incubator at 30°C for 2 days, stored in a refrigerator at 6°C, and used within 1 day.

[0027] B. Preparation of seed solution

[0028] In an aseptic operating environment, the strains on the slant medium were transferred to the seed medium (universal fermentation medium). The composition of the seed medium (g / L): glucose 22, urea 0.6, yeast extract 0.6, K 2 HPO 4 6. KH 2 PO 4 3, NaCl 0.2, (NH 4 ) 2 SO 4 0.3, MgSO 4 ·7H 2 O 0.3, pH7.2, sterilized (121°C, 30min). The inoculated seed medium was cultured in a shaker incubato...

Embodiment 2

[0040] A. Inclined preservation and transfer of strains

[0041] Composition (g / L) of the slant culture medium for preserved strains: beef extract 3, agar 15, peptone 8, NaCl 4, pH 7.0, sterilized (115° C., 20 min). In an aseptic operating environment, the strains of Proteus mirabilis were placed on the slant medium, cultured in a constant temperature incubator at 25°C for 1 day, stored in a refrigerator at 2°C, and used within 1 day.

[0042] B. Preparation of seed solution

[0043] In an aseptic operating environment, the strains on the slant medium were transferred to the seed medium (universal fermentation medium). Composition of seed medium (g / L): glucose 18, urea 0.4, yeast extract 0.4, K 2 HPO 4 4. KH 2 PO 4 1, NaCl 0.05, (NH 4 ) 2 SO 4 0.1, MgSO 4 ·7H 2 O 0.1, pH 6.8, sterilized (115°C, 20min). The inoculated seed medium was cultured in a shaker incubator at a temperature of 20°C and a shaker speed of 150r / min for 2 days, stored in a refrigerator at 2°C, ...

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Abstract

This invention relates to a preparation method of using proteus mirabilis to prepare biofloculation agent. The craft as follows: (1) subcuitivate Proteus mirabilis to slant culture group, preserve at freezer of 2 to 6 degC;(2) inoculate conservative strain to seed culture group, then prepare seed liquid in rocker incubator at 25 to 30 degC; (3) by proportion of 1 to 2% inoculate seed liquid to sixpenny nutrient medium, then embed it in the rocker, at 25 to 30degC, 120 to 140r/min, 1 to 2 days to obtain strain nutrient medium fermentation broth, take centrifugalization 20 to 40 min by 4000 to 6000r/ min centrifuge, remove sediment, the supernatant liquid is liquid biofloculation agent, measure flocculation ratio; (4) abstract and purify the liquid biofloculation to gain product. This invention has low cost, short production cycle, flocculation ratio can reach 92.88 to 93.13%, and has no secondary pollution, possess good generalization value.

Description

technical field [0001] The invention relates to a preparation method of a biological flocculant, in particular to a method for preparing a biological flocculant by using Proteus mirabilis. Background technique [0002] Flocculants are substances that can coagulate and precipitate suspended particles that are not easy to settle in liquids. They are widely used in industrial water treatment, domestic sewage treatment, food production and fermentation industries. Flocculants can generally be divided into three categories: ①Inorganic flocculants, such as aluminum sulfate, polyaluminum chloride, ferrous sulfate and alum; ②Synthetic organic polymer flocculants, such as polyethylene oxide, polystyrene sulfonate , polyacrylamide derivatives and polyethyleneimine, etc.; ③ natural organic polymer flocculants, such as cellulose, starch, polyglucosamine, sodium alginate and biological flocculants. Many inorganic flocculants are widely used due to their good flocculation effect and low ...

Claims

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Application Information

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IPC IPC(8): C02F1/52C12N1/20C12R1/37
Inventor 夏四清王学江张志强杨阿明
Owner TONGJI UNIV
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