Degumming process for mulberry fibre by bacilli compound bacteria
A technology of complex flora and mulberry fiber, applied in the direction of microbial-based methods, bacteria, bacterial retting, etc., can solve the problems of not conforming to the harmonious development of ecological protection, chemical degumming method, high energy consumption, low fiber output rate, etc. , to achieve good degumming effect, mild degumming conditions and low residual glue rate
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Embodiment 1
[0023] 1. Enrichment medium composition (w / v): 5% dry mulberry bark, 0.5% (NH 4 ) 2 SO 4 .
[0024] Screening method: Collect soil samples from biogas pond mud, pond bottom mud, woods, banana fields and other places rich in pectin, place them in enriched medium, culture statically at 37°C and pH 8.0, observe the release of fibers and Measure the content of pectinase, xylanase and ligninase in the culture medium, select the culture with the best degumming effect and high enzyme content, take 50ml of the culture medium, transfer it to 450ml of new enrichment medium, and keep at 37°C Static culture, after 5 times of enrichment, the bacterial complex flora with good degumming effect was obtained.
[0025] 2. Cultivation of degummed bacterial complex flora
[0026] Composition (w / v) of the culture medium: pectin 0.5%, urea 0.2%.
[0027] Cultivation process conditions: Inoculate the screened degummed bacterial complex flora into the degummed bacterial complex flora culture sol...
Embodiment 2
[0035] 1. Screening of degummed bacterial complex flora
[0036] The same method as in Example 1 was used for enrichment and screening.
[0037] 2. Cultivation of degummed bacterial complex flora
[0038] Composition of culture medium (w / v): 0.5% xylan, 0.5% KNO 3 .
[0039] Cultivation process conditions: the screened degummed bacterial complex flora is inoculated into the degummed bacterial complex flora culture solution, and cultured at 35° C. and pH 7.0 for 48 hours to obtain the degummed bacterial complex flora seed liquid.
[0040] 3. Degumming
[0041] Composition of degumming solution (w / v): 0.5% (NH 4 ) 2 SO 4 solution.
[0042] Degumming process: add the degumming solution in the dry mulberry bark by dry mulberry bark: the ratio of degumming solution is 1: 25, insert the degummed bacterial complex flora seed solution cultivated by the inoculation amount of 15% (v / v), 40 ℃, pH8.0 soaking for 48 hours for degumming.
[0043] 4. Rinsing and drying process
[0...
Embodiment 3
[0047] 1. Screening of degummed bacterial complex flora
[0048] The same method as in Example 1 was used for enrichment and screening.
[0049] 2. Cultivation of degummed bacterial complex flora
[0050] Composition (w / v) of the culture solution: xylose 0.2%, urea 0.5%.
[0051] Cultivation process conditions: Inoculate the screened degummed bacterial complex flora into the degummed bacterial complex flora culture solution, and cultivate at 35° C. and pH 7.5 for 36 hours to obtain the degummed bacterial complex flora seed culture solution.
[0052] 3. Degumming
[0053] Degumming solution composition (w / v): 0.2% urea solution.
[0054] Degumming process: Add the degumming solution to the dry mulberry bark according to the ratio of dry mulberry bark: degumming solution is 1:30, insert the degumming bacterial complex flora seed solution according to the inoculation amount of 15% (v / v), 35 ° C, pH8 .5 Soak for 60 hours for degumming.
[0055] 4. Rinsing and drying process
...
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