Molecular biological quantitative detecting method for pathogenicity paratypic hemolytic vibrio in aquatic products

A quantitative detection method, molecular biology technology, applied in biochemical equipment and methods, microbial determination/inspection, resistance to vector-borne diseases, etc. question
CN1831139AInactive Publication Date: 2006-09-13OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2006-09-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method to test pathogenicity vibrio parahaemolyticus in aquatic product that the feature is including the following steps: designing, filtering, and manufacturing the specificity primer of the testing strain, expanding PCR and testing, distilling DNA, PCR expanding of the testing strain specificity gene section, agarose gel electrophoresis of the PCR product and testing the specificity section; MPN method testing and calculating the testing result. The invention discloses a method to rapidly testing bacilli in testing sample.
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Description

technical field

[0001] The invention belongs to a microorganism detection method, in particular to a molecular biology method for rapid and quantitative detection of pathogenic Vibrio parahaemolyticus in aquatic products.

[0002] Scope of application: This method is suitable for rapid quantitative detection of any microorganisms with specific enrichment medium, specific gene fragments with strong specificity, and specific primers can be designed. Background technique

[0003] Vibrio parahaemolyticus is a pathogenic halophilic bacteria widely distributed in oceans and salt lakes. It has been paid attention to because it can cause food poisoning, especially in coastal areas in summer and autumn, often due to eating seafood containing a large amount of Vibrio parahaemolyticus, causing explosive food poisoning; in non-coastal areas, eating food contaminated by this bacterium Salted food is also often poisoned; it is a mandatory inspection item in commodity inspection and quara...

Claims

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