The invention discloses
a DNA library quantifying and sequencing method. The method comprises the steps that step 1, preparation of
processing a sheet, a sample and a
reagent is performed; step 2, a 1.5-milliliter low-adsorption tube is prepared, 499 microliter of NF water is added, and number and NF
water volume are verified; a melt sample is vibrated, evenly mixed and transiently centrifuged tothe bottom of the tube, 1 microliter of sample is added into 499 microliter of NF water,
tube number is verified, the diluted sample is vibrated, evenly mixed and transiently centrifuged; MIX is prepared on an ice box, 18 microliter of MIX is put into a 0.2-milliliter PCR plate placed on a
metal ice box; number is verified, 2 microliter of sample is extracted and put into 18 microliter of MIX; after film sealing is carried out,
centrifugation is carried out, and detection is performed in a PCR instrument; step 3, according to a quantifying detection result in step 2, an instrument-on sheet ismade, and
emulsion PCR is carried out; step 4, OT instrument-off
processing is carried out; and step 6, instrument-on sequencing is carried out. The
DNA library quantifying and sequencing method has the advantages that the operation is simple, convenient and flexible, the sequencing cost is reduced, the sequencing processes are reduced, the labor efficiency is improved, the processes are scientific, the standard is clear, and the practical operation effect is good.