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Bacteria culture medium and preparation method thereof

A technology of bacterial culture medium and culture medium, applied to bacteria and other directions, can solve the problems of inability to directly observe and calculate measurement results, cumbersome detection procedures, etc., and achieve the effects of easy observation of detection results, simple and convenient operation, and reasonable process design.

Inactive Publication Date: 2015-06-10
HAIMEN RUNSHENG TEXTILE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above-mentioned background technology, there are the shortcomings of cumbersome detection procedures, inability to directly observe and calculate the measurement results

Method used

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Examples

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Embodiment Construction

[0021] The following examples will further illustrate the present invention, but do not limit the present invention thereby.

[0022] A bacterial culture medium, which uses nutrient agar medium as a base medium, is characterized in that a water-retaining agent and a stain are added to the base medium, wherein the weight ratio of nutrient agar: water: water-retaining agent: stain is 37 -39: 1000: 0.2-4: 0.02-0.09.

[0023] Further, the weight ratio of nutrient agar: water: water retaining agent: dyeing agent is 37-39: 1000: 0.3-4: 0.02-0.08.

[0024] Preferably, the water-retaining agent is selected from starch graft copolymerized acrylic acid superabsorbent, starch grafted copolymerized acrylate superabsorbent, modified cellulose superabsorbent resin, or propylene derivatives and inorganic substances to form a superabsorbent resin, dyed Carbofuran, Congo red, carbolic sargassum red, crystal violet, methyl blue or red tetrazolium are selected as the agent.

[0025] A preparat...

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PUM

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Abstract

The invention discloses a bacteria culture medium and a preparation method thereof. A nutrient agar medium is taken as a basic culture medium, wherein a water-retaining agent and a coloring agent are added in the basic culture medium, the weight ratio of the nutrient agar, the water, the water-retaining agent to the coloring agent is (37-39):1000:(0.2-4): (0.02-0.09); and during the preparation process, each component is weighed, mixed and sterilized. By adopting the water-retaining agent in the obtained bacteria culture medium, the moisture of the culture medium can be kept and the accuracy of the measuring result influenced by the water loss and dryness can be avoided; by adopting the coloring agent, most bacterial colonies are dyed red or pink so that the detecting result can be conveniently observed; and simultaneously the preparation method is reasonable in design and simple and convenient in operation.

Description

technical field [0001] The invention relates to a bacterial culture medium and a preparation method thereof, in particular to a bacterial culture medium and a preparation method thereof, and belongs to the field of microbiology culture medium preparation. Background technique [0002] The ingredients of natural medium are all composed of natural products, such as animals, plants or microorganisms including their extracts. The compound medium is composed of some natural products and some compounds with known components. Synthetic media consist of compounds of known composition, such as combinations of pure compounds. The medium can be divided into solid medium, semi-solid medium and liquid medium according to its physical form. The medium can also be divided into basal medium, enriched medium, differential medium and selective medium according to its use. Basal media provide the most basic nutrients. Rich medium is the addition of more nutrients to the basic medium to ach...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 戴祖新
Owner HAIMEN RUNSHENG TEXTILE
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