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Microbial agent for preventing and treating pinellia ternate root rot as well as preparation method and application thereof

A technology for root rot and pinellia root rot, which is applied in the field of prevention and control of root rot of Pinellia pinellia and its preparation, can solve problems such as the undiscovered Bacillus pinellia pinellia root rot, and achieve remarkable control effects

Active Publication Date: 2019-04-05
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After retrieval, there is no report on the prevention and treatment of pinellia root rot with Bacillus velezensis

Method used

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  • Microbial agent for preventing and treating pinellia ternate root rot as well as preparation method and application thereof
  • Microbial agent for preventing and treating pinellia ternate root rot as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A bacterial strain for preventing and treating pinellia root rot, Bacillus velezensis MES861, date of deposit: December 3, 2018, name and abbreviation of the depository unit: China General Microorganism Culture Collection Center (CGMCC), Deposit number: CGMCC No.16857.

[0034] The preparation of Bacillus Velez MES861 microbial bacterium liquid is carried out according to the following steps

[0035] (1) Liquid shake flask culture medium: nutrient agar (the nutrient agar includes 10g peptone, 3g beef extract, 5g sodium chloride, 1000mL water, pH value is 7.0), the prepared medium is sterilized by autoclaving at 121°C for 20 minutes Then cool it down for later use.

[0036] (2) Pick Bacillus Veles and inoculate it into the liquid nutrient agar shake flask culture medium described in 100mL step (1). Stand by after 1 hour to prepare the Bacillus velei fermented liquid. The fermented liquid obtained by fermenting the bacillus velei is a fungal agent for preventing and tr...

Embodiment 2

[0038] A bacterial strain for preventing and treating pinellia root rot, Bacillus velezensis MES861, date of deposit: December 3, 2018, name and abbreviation of the depository unit: China General Microorganism Culture Collection Center (CGMCC), Deposit number: CGMCC No.16857.

[0039] The preparation of Bacillus Velez MES861 microbial bacterial powder is carried out according to the following steps

[0040] (1) Liquid shake flask medium: nutrient agar (5g peptone, 1g beef extract, 5g sodium chloride, 1000mL water, pH value 7.0), the prepared medium is sterilized by high pressure at 126°C for 30 minutes and cooled for later use .

[0041] (2) Pick Bacillus Velez and inoculate it into the liquid nutrient agar shake flask culture medium described in 100mL step (1). Stand by after 1 hour to prepare the Bacillus velei fermented liquid. Spray-dry the fermented liquid obtained by the fermentation of Bacillus velei. The spray-drying process is a commonly used preparation process in...

Embodiment 3

[0043] A bacterial strain for preventing and treating pinellia root rot, Bacillus velezensis MES861, date of deposit: December 3, 2018, name and abbreviation of the depository unit: China General Microorganism Culture Collection Center (CGMCC), Deposit number: CGMCC No.16857.

[0044] The preparation of Bacillus Velez MES861 microbial bacterium liquid is carried out according to the following steps

[0045](1) Liquid shake flask medium: Nutrient agar (10g peptone, 3g beef extract, 5g sodium chloride, 1000mL water, pH value 7.0), the prepared medium is autoclaved at 123°C for 15 minutes and then cooled for later use .

[0046] (2) Pick Bacillus Veles and inoculate it into the liquid nutrient agar shake flask culture medium described in 100mL step (1). Stand by after 1 hour to prepare the Bacillus velei fermented liquid. The fermented liquid obtained by fermenting the bacillus velei can obtain the bacterial strain for preventing and treating pinellia root rot.

[0047] 2. Fu...

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Abstract

The invention relates to a microbial agent for preventing and treating pinellia ternate root rot as well as a preparation method and application thereof, in particular to bacillus velezensis MES861. According to the bacillus velezensis MES861, the preservation date is December 03, 2018, the name and the abbreviation of the preservation organization are China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.16857. According to the pinellia ternate root rot prevention and treatment microbial agent, fermentation liquor prepared through fermentation of the bacillus velezensis serves as the microbial agent for preventing and treating the pinellia ternate root rot. The preparation method for the microbial agent for preventing and treating the pinellia ternate root rot comprises the step that a liquid nutrient agar culture medium is inoculated with the bacillus velezensis, and shake culture is carried out so as to obtain the bacillus velezensis fermentation liquor. The bacillus velezensis has a strong inhibition effect on pathogenic bacteria such as fusarium graminearum, fusarium solani, fusarium oxysporum and F.moniliforlle, so that the occurrence of the pinellia ternate root rot is effectively prevented, and the stable yield and the income increase of pinellia ternate are promoted.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and relates to a fungal agent for preventing and controlling pinellia root rot and its preparation method and application. Background technique [0002] Pinellia root rot is a fungal soil-borne disease. The fungus develops rapidly. In mild cases, 80% of the plants will rot within a week, and in severe cases, the entire Pinellia plot will be infected and rot and die within 3-5 days, especially for long-term planting pinellia. The plots are more difficult to control, causing serious losses to pinellia production. [0003] The traditional control method is to choose medium loam or loam soil with better soil quality, cooperate with intertillage to improve soil compaction, and rotate with gramineous crops. The operation is more complicated. Commonly used chemical agents are carbendazim, mancozeb, Rick and so on. Long-term extensive use of chemical fertilizers and pestici...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 赵钢勇肖培英顾欣燕刘刚刘金龙赵晓琛
Owner 天津开发区坤禾生物技术有限公司
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