Method for rapidly detecting antibacterial properties of materials
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A detection method and performance technology, applied in the field of rapid detection of antibacterial properties of materials, can solve the problems of long detection period, affecting the rapid development of antibacterial technology, and complicated operation.
Inactive Publication Date: 2018-12-14
浙江省产品质量安全检测研究院 +1
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Problems solved by technology
In the process of research and development of antibacterial materials, it is inevitable to use the method of antibacterial performance detection. The current detection method of antibacterial activity of materials has the disadvantages of long detection period and comp...
Method used
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Embodiment 1
[0016] 1. Chromogenic agent: 2,3,5-triphenyltetramlium chloride (2,3,5-triphenyltetramlium chloride, abbreviated as TTC), accurately weigh 1.00g TTC and dilute to 100mL brown volumetric flask, Prepare a working solution with a concentration of 1mg / mL, sterilize it at 121°C for 15min, and cool it for later use.
[0017] 2. Test strain: Escherichia coli ATCC 25922, take the 3rd to 5th generation nutrient agar medium slant for 18-24h fresh culture, wash the slant with 5mL 0.03mol / L phosphate buffer solution repeatedly, wash off the bacterial lawn, Shake to mix, dilute to 10 6 ~10 7 CFU / mL, stored in a 4°C refrigerator for later use.
[0018] 3. Test materials: Take the control sample and the test sample (the antibacterial rate > 95% tested by the standard method), wipe the surface with 70% ethanol solution, rinse with sterile water after 5 minutes, and dry naturally.
[0019] 4. Bacterial inoculation and shaking culture: Take the control sample material and the tested sample m...
Embodiment 2
[0024] 1. Chromogenic agent: 2,3,5-triphenyltetramlium chloride (2,3,5-triphenyltetramlium chloride, abbreviated as TTC), accurately weigh 1.00g TTC and dilute to 100mL brown volumetric flask, Prepare a working solution with a concentration of 1mg / mL, sterilize it at 121°C for 15min, and cool it for later use.
[0025] 2. Test strain: Staphylococcus aureus, take the 3rd to 5th generation nutrient agar medium slant for 18-24h fresh culture, wash the slant repeatedly with 5mL 0.03mol / L phosphate buffer, wash off the bacterial lawn, shake and mix well , diluted to 10 6 ~10 7 CFU / mL, stored in a 4°C refrigerator for later use.
[0026] 3. Test materials: Take the control sample and the test sample (the antibacterial rate > 95% tested by the standard method), wipe the surface with 70% ethanol solution, rinse with sterile water after 5 minutes, and dry naturally.
[0027] 4. Bacterial inoculation and shaking culture: Take the control sample material and the tested sample material...
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Abstract
The invention relates to a method for rapidly detecting antibacterial properties of materials and belongs to the technical field of material detection. The method comprises the following steps: takinga nutrient agar medium slant fresh culture of a test strain, repeatedly washing the slope by using a PBS (Phosphate Buffered Solution), washing lawn, oscillating and uniformly mixing, diluting and storing in a refrigerator at the temperature of 4 DEG C for later use; wiping surfaces of a control sample material and a detected sample material by using a 70% ethanol solution, washing by using sterile water and naturally drying; putting the control sample material and the detected sample material into a triangular flask, adding 95mL of PBS, uniformly mixing, adding 5mL of a pre-made bacterial suspension and carrying out oscillating contact treatment; and adding a 2,3,5-triphenyltetramlium chloride (TTC) standard solution into the bacteria solution after the oscillating contact treatment, oscillating in the dark, centrifuging 10mL of the bacteria solution, adding 10mL of acetone into the precipitate to carry out extraction, centrifuging the extract, determining OD485 through the supernatant and calculating the antibacterial rate according to a TTC standard curve. The method disclosed by the invention has a broad application prospect when serving as a screening method for qualitativelydetecting antibacterial activity of the materials.
Description
technical field [0001] The invention belongs to the technical field of material detection, and in particular relates to a rapid detection method for antibacterial properties of materials. Background technique [0002] With the development of science and technology, antibacterial technology shows a relatively broad application prospect, and is gradually applied to many fields such as packaging materials, daily chemical products, sterilizing appliances, antibacterial coatings, medical and health care, and the development and application of new antibacterial materials. research hotspot. In the process of research and development of antibacterial materials, it is inevitable to use antibacterial performance detection methods. The current detection methods for antibacterial activity of materials have disadvantages such as long detection cycle and complicated operation. The backwardness of antibacterial activity detection methods of materials has greatly affected the rapid develop...
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