A method for producing epothilone b

Abstract

The invention concretely discloses a fermentation method for producing epothilone B, and belongs to the field of biological fermentation. According to the method, during culturing of a seeding tank, ammonia hydroxide is added in a flowing mode to adjust the pH value, so that a lot of seed thalli are obtained; during fermentation, ammonia hydroxide or propanoic acid is added in a flowing mode to regulate the pH value, in this way, the yield of the epothilone B is increased, the regulation procedures are simplified, production cost is lowered, industrialized production is easy, and application prospects are good.

Description

technical field

[0001] The invention belongs to the field of biological fermentation, and in particular relates to a method for producing epothilone B. Background technique

[0002] Epothilones (epothilones) was discovered in 1993 in the secondary metabolites of the myxobacteria Sorangium cellulosum, and its structure is a macrolide compound. The anti-tumor mechanism is similar to that of paclitaxel, which has the effect of inhibiting microtubule depolymerization. In contrast, epothilone is more water-soluble and has a strong effect on both multidrug-resistant tumor cells and paclitaxel-resistant tumor cells. It has anticancer activity and does not have serious side effects such as granulocytopenia, peripheral neuropathy, alopecia, and allergy in the clinical application of paclitaxel. Therefore, the development of epothilones has clinical value and social significance. In 2007, ixabepilone, a derivative of epothilones B, was approved for marketing in the United States, wh...