Fermentation method of L-lysine

A fermentation method, lysine technology, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of slow growth, reduced economic benefits, slow growth of bacteria, etc.

Active Publication Date: 2021-04-09
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY +1
View PDF7 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the conventional L-lysine fermentation, due to the high concentration of the culture medium in the early stage of fermentation, excessive osmotic pressure is generated on the bacteria, which makes the growth of the bacteria slow and the fermentation period is prolonged.
In the middle and late stages of fermentation, the nutrients in the f

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fermentation method of L-lysine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for producing L-isoleucine by fermentation, the specific method is as follows:

[0025] (1) Inoculate the L-lysine-producing bacteria Corynebacterium glutamicum in a bacterial preservation tube stored at -80°C on a slant medium for activation, and culture at a constant temperature of 32°C for 12 hours.

[0026] (2) Inoculate the bacteria on the slope into three 1L Erlenmeyer flasks, which contain 100mL of primary seed medium, adjust the pH to 7.0-7.2 with ammonia water, shake at 220rpm / min, and cultivate at a constant temperature of 32°C for 8-9h. OD 600 Get to around 20.

[0027] (3) Connect three 100mL primary seed liquids and 100mL 80% glucose into a 5L fermenter, which contains 2.6L seed culture medium, and control the fermentation temperature at 32°C, dissolved oxygen at 30-50%, and pH7. 0, when the dissolved oxygen is lower than 30%, increase the speed and air volume. Grow to OD 600 ≥25, seed age 21-23h.

[0028] (4) Release the seed liquid to the re...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a fermentation method of L-lysine. According the invention, nutrient division fermentation and high-density fermentation are combined, a fermentation medium is prepared according to a ratio of 4: 6, 40% as a fermentation base material and 60% into a concentrated fermentation medium, fed batch is started 2 h after the beginning of fermentation and stopped 4 h before the end of fermentation, and the concentration of the initial fermentation medium is reduced to prevent the excessive osmotic pressure of bacteria due to excessive nutrition, which inhibit the growth of the bacteria. Nutrient division fermentation started 2 h after the beginning of fermentation makes up for nutrient deficiency and imbalance in the bacteria growth process, so that the bacteria activity is improved, the bacteria density in fermentation liquor is increased, the bacteria amount is increased by about 20%, the fermentation period is shortened to 40 h from 44 h, and the cost is reduced; in the fermentation process, balanced nutrition supplementation enables metabolic flow to flow to the L-lysine more, the conversion rate is increased from 70% to 73%, generation of byproducts such as acetic acid and alanine is effectively reduced, and the yield of the L-lysine is increased from 223 g/L to 261 g/L.

Description

technical field [0001] The invention relates to the field of amino acid fermentation, in particular to a fermentation method of L-lysine. Background technique [0002] L-Lysine is one of the eight essential amino acids, widely used in the feed industry, food and pharmaceutical industries, of which about 90% are used in feed additives. Compared with other essential amino acids, L-lysine content in grains is the lowest, so it can be used to balance the proportion of amino acids in feed and improve the absorption of grain protein in the body. In the food industry, L-lysine is mainly used in food nutrition enhancers, deodorants and coloring agents. In the pharmaceutical industry, L-lysine is the raw material for the synthesis of hormones, enzymes, antibodies and various amino acid infusions. [0003] In conventional L-lysine fermentation, due to the high concentration of the culture medium in the early stage of fermentation, excessive osmotic pressure is generated on the bacte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P13/08C12N1/20C12R1/15
CPCC12P13/08C12N1/20
Inventor 徐庆阳孙际宾刘云鹏郑平李燕军周文娟张成林陈宁
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap