Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles

A technology for water purification and bacteria, applied in chemical instruments and methods, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc. External environmental damage, poor mechanical properties of particles, etc., to achieve the effect of reducing bacterial loss, shortening start-up time, and enhancing stability

Active Publication Date: 2015-09-09
CENT LAB FUJIAN ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the existing preparation method of bacteria-immobilized granules is difficult to realize industrialized production. Poor performance, weak settling performance, easy loss, etc., provide a kind of bacteria-immobilized particles for water purification and its preparation method, the bacteria-immobilized particles prepared by the method of the present invention can e

Method used

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  • Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles
  • Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles
  • Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles

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[0036] Example 1

[0037] Bacterial liquid: Bacillus subtilis liquid.

[0038] The preparation method of Bacillus subtilis immobilized particles includes the following preparation steps:

[0039] (1) Add 1.6 kg of activated carbon and 0.4 kg of diatomaceous earth to 18 L of the bacterial liquid in the late logarithmic growth period, stir and absorb for 20 min, to prepare the Bacillus subtilis bacterial liquid;

[0040] (2) Add 160 mL of 12wt% chitosan acetic acid solution to the microbial broth prepared in step (1), stir quickly for 20 minutes, then slowly stir for 30 minutes, then let stand for 6 hours, and then suck up with a siphon The supernatant was made into a 3.8 kg bacterial concentrate;

[0041] (3) Add 2.4 kg of diatomaceous earth, 0.8 kg of sodium bentonite and 0.8 kg of silica to the bacterial concentrate prepared in step (2), and stir for 20 minutes;

[0042] (4) Add 6.24 L (15wt% PVA + 0.75wt% SA) mixed solution to the bacterial mixture obtained in step (3), stir the mate...

Example Embodiment

[0049] Example 2

[0050] The microorganism liquid is a mixture of Bacillus subtilis, Bacillus thuringiensis, yeast, and photosynthetic bacteria.

[0051] The preparation method of mixed bacteria immobilized particles includes the following preparation steps:

[0052] (1) Add 200 g of activated carbon and 50 g of diatomaceous earth to 2L of the bacterial mixture in the late logarithmic growth period (the mixing ratio of Bacillus subtilis, Bacillus thuringiensis, yeast, and photosynthetic bacteria is 2:1:1:1) In the medium, stirring and adsorbing for 20 minutes, the microorganism liquid is prepared;

[0053] (2) Add 20 mL of 12wt% chitosan acetic acid solution to the microbial broth prepared in step (1), stir quickly for 20 minutes, then slowly stir for 30 minutes, let it stand for 6 hours, and then suck it up with a siphon. Clear liquid to make 450 g bacterial concentrate;

[0054] (3) Add 300 g of diatomaceous earth, 100 g of sodium bentonite and 100 g of silicon dioxide to the bac...

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Abstract

The invention discloses bacteria immobilization particles for water purification and a preparation method of the bacteria immobilization particles, and belongs to the technical field of water treatment. The preparation method comprises the following steps: preparing a bacteria concentrate through flocculation by utilizing activated carbon, diatomite and a chitosan acetic acid solution; adding diatomite, bentonite, silicon dioxide and a polyvinyl alcohol-sodium alginate mixed solution into the concentrate for stirring; carrying out granulation with a granulator to prepare the bacteria immobilization particles. The prepared bacteria immobilization particles are high in biological activity, high in bacteria content (5*10<9>-1*10<10> CFU/g), short in starting time, and high in reaction speed, has a honeycomb structure (100-300 m<2>/g in specific surface area) suitable for bacteria apposition growth and mass transfer, and can be used for immobilizing bacteria for long, prohibiting the invasion of external hazardous materials, and permanently and efficiently treating sewage in different environments and of different water quality types; meanwhile, industrialized production is available for a whole bacteria immobilization particle preparation process, and the prepared bacteria immobilization particles are high in mechanical property, long in service life, and convenient to store and convey.

Description

technical field [0001] The invention belongs to the technical field of water treatment, in particular to a bacteria-immobilized particle for water purification and a preparation method thereof. Background technique [0002] Immobilized microorganism technology is a method that uses physical or chemical means to limit free microbial cells to a certain space area, so that microorganisms can maintain high density and activity and can be used repeatedly. Compared with the traditional free microorganism treatment method, the immobilized microorganism technology can greatly increase the concentration of microorganisms, so that the microorganisms are not easy to be lost and polluted, the use time is long, the anti-toxicity and tolerance are significantly increased, and the solid-liquid separation is easy, and the secondary pollution is less. . These advantages make immobilized microorganism technology have broad application prospects in the field of water treatment. [0003] Curr...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/04C02F3/34
Inventor 黄薇宋永康林代炎陈丽华刘文静王命福
Owner CENT LAB FUJIAN ACADEMY OF AGRI SCI
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