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A technology of nutrient flow and glutamic acid, which is applied in the field of amino acid fermentation, can solve the problems of affecting the production of L-glutamic acid, affecting the performance of L-glutamic acid, and the decline of bacterial vitality, so as to speed up the growth rate and stabilize the metabolic flow , the effect of increasing the amount of bacteria
Pending Publication Date: 2021-04-23
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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Problems solved by technology
However, when producing according to the existing production method, the initial temperature-sensitive Corynebacterium glutamicum growth rate is relatively slow OD 600 It takes a long time to reach 20~24, the remaining growth is less, and the amount of bacter
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Embodiment 1
[0019] (1) Bacteria activation: temperature-sensitive Corynebacterium glutamicum (GKG-047, storage number CGMCC No.5481) stored in a -80°C refrigerator was inoculated on a slant and activated twice.
[0020] The slant medium is peptone 5 g / L, beef extract 10 g / L, yeast powder 4 g / L, corn steep liquor dry powder 25 mL / L, KH 2 PO 4 1 g / L, MgSO 4 0.2 g / L, NaCl1 g / L, agar powder 25 g / L, methionine 0.2 g / L, pH=6.8~7.0;
[0021] (2) Seed culture: elute the activated strain with sterile water, and inoculate all the strains into the prepared seed fermentation broth for seed culture after elution.
[0022] The seed medium is: glucose 40 g / L, corn steep liquor powder 10 g / L, KH 2 PO 4 2. g / L, MgSO 4 ·7H 2 O0.8 g / L, MnSO 4 5 mg / L, FeSO 4 5 mg / L, threonine 1 g / L, V H 10mg / L.
[0023] The seed culture conditions are as follows: the temperature is maintained at about 32°C, the dissolved oxygen is controlled at 20-40%, and the pH is controlled at about 7.0 by ammonia water.
[002...
Embodiment 2
[0030] With reference to Example 1, the difference is that when the thalline reaches 12, the full nutrient medium that starts to add volume fraction is 60%.
Embodiment 3
[0032] With reference to Example 1, the difference is that when the thallus reaches 18, the full nutrient medium that starts to add volume fraction is 60%.
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Abstract
The invention belongs to the technical field of amino acid fermentation, and discloses a total-nutrient feeding high-density fermentation method for producing L-glutamic acid by fermenting temperature-sensitive corynebacterium glutamicum. The total-nutrient feeding high-density fermentation method comprises the steps of: inoculating the temperature-sensitive corynebacterium glutamicum into fermentation liquor, and continuously feeding a total-nutrient culture medium into the fermentation liquor when the temperature-sensitive corynebacterium glutamicum grows to a logarithmic phase, wherein the total-nutrient culture medium comprises inorganic salt ions such as sylvite, magnesium salt and the like and various growth factors required for growth of other temperature-sensitive corynebacterium glutamicum. According to the method, the total-nutrient culture medium is fed at the logarithmic phase of thalli, and the fed total-nutrient culture medium can timely make up nutrient substances consumed by growth and production of the thalli, so that the thalli are always in an optimal fermentation environment, and the thalli density in the fermentation liquor is improved; and meanwhile, in the later period of fermentation, feeding of the total-nutrient culture medium also can improve the activity of the thalli and ensure the production performance of the thalli so as to improve the yield of the L-glutamic acid.
Description
technical field [0001] The invention belongs to the technical field of amino acid fermentation, and relates to a method for high-density fermentation of L-glutamic acid with full-nutrition fed-batch feeding, in particular to a full-nutrient fed-batch feeding method for producing L-glutamic acid by fermentation of temperature-sensitive Corynebacterium glutamicum High density fermentation method. Background technique [0002] At present, my country's monosodium glutamate industry is developing rapidly, and its output ranks first in the world. The annual demand has reached 1.19 million tons. , The market potential is huge, which in turn drives the development of the glutamic acid industry. According to statistics, the national glutamic acid output is nearly 3 million tons, and the production scale has a tendency to further increase. [0003] Temperature-sensitive Corynebacterium glutamicum is an emerging strain of L-glutamic acid production, which can change the cell membrane...
Claims
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IPC IPC(8): C12P13/14C12R1/15
Inventor 赵兰坤徐庆阳孙钦波刘景阳李树标王峰赵凤良王小平时夫龙程士清
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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