Additive for synergetically promoting multiplication and colonization of intestinal probiotics and use method of additive

A technology of intestinal probiotics and additives, applied in application, additional food elements, animal feed, etc., to achieve the effect of shortening the lag period, improving the level of adhesion receptors, and strong competitiveness

Pending Publication Date: 2017-06-20
QILU UNIV OF TECH +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned above, there are few reports on the synergistic effect of xylooligosaccharides, Clos

Method used

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  • Additive for synergetically promoting multiplication and colonization of intestinal probiotics and use method of additive
  • Additive for synergetically promoting multiplication and colonization of intestinal probiotics and use method of additive
  • Additive for synergetically promoting multiplication and colonization of intestinal probiotics and use method of additive

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Embodiment 1: an additive for synergistically promoting the proliferation and colonization of intestinal probiotics, which is made of the following raw materials in parts by weight: 10-15 parts of xylooligosaccharides, 10-15 parts of Clostridium butyricum, High fulvic acid sodium humate 70-80 parts. The xylooligosaccharide refers to the content of xylooligosaccharide with a degree of polymerization of 2-4 is more than 55% (the product is produced by Shandong Longli Biotechnology Co., Ltd.). The content of fulvic acid in the high fulvic acid sodium humate is ≥12%. The number of live bacteria of Clostridium butyricum is 10 10 -10 11 Colonies / g.

[0037] Preparation of high fulvic acid sodium humate:

[0038] (1) Preparation of mycelia liquid: Phanerochaete chrysosporium (purchased from China Industrial Microorganism Culture Collection Management Center, No. CICC 40299, Phanerochaete chrysosporium ) was inoculated into the wort liquid medium, and cultured at 39 degree...

Example Embodiment

[0048] Embodiment 2: an additive for synergistically promoting the proliferation and colonization of intestinal probiotics, which is made of the following raw materials in parts by weight: 10-15 parts of xylooligosaccharides, 10-15 parts of Clostridium butyricum, High fulvic acid sodium humate 70-80 parts. The xylooligosaccharide refers to the content of xylooligosaccharide with a degree of polymerization of 2-4 is more than 55% (the product is produced by Shandong Longli Biotechnology Co., Ltd.). The content of fulvic acid in the high fulvic acid sodium humate is ≥12%. Preparation of high fulvic acid sodium humate:

[0049] (1) Preparation of mycelia liquid: Phanerochaete chrysosporium (purchased from China Industrial Microorganism Culture Collection Management Center, No. CICC 40299, Phanerochaete chrysosporium ) was inoculated into the wort liquid medium, and cultured at 39 degrees Celsius for 6 days to prepare the mycelia liquid.

[0050] (2) Mix the mycelia liquid, pur...

Example Embodiment

[0059] Embodiment 3: The similarities between this embodiment and Embodiment 2 will not be repeated, the difference is: the preparation of high fulvic acid sodium humate

[0060] (1) Preparation of mycelial liquid: Phanerochaete chrysosporium was inoculated into wort liquid culture medium, and cultured at 39 degrees Celsius for 8 days to prepare mycelial liquid.

[0061] (2) Mix the mycelia liquid, purified water and sodium humate (produced by Jinan Haihua Biotechnology Co., Ltd.) according to the weight of 3:2:4, and add inorganic salts (urea 0.3%, dipotassium hydrogen phosphate 0.5% according to the weight of the mixture) , potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.3%, manganese sulfate 0.05%).

[0062] (3) The pH is controlled at 6.0, the temperature is 37 degrees Celsius, and the reaction is stirred at 60 rpm for 8 days.

[0063] (4) The reaction solution was dried by a drum to obtain a flake product with a fulvic acid content of 14.4%.

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Abstract

An additive for synergistically promoting the proliferation and colonization of intestinal probiotics and its use method, which is composed of xylo-oligosaccharides, Clostridium butyricum and high fulvic acid sodium humate, and xylo-oligosaccharides provide nutrition for Clostridium butyricum , to promote the proliferation of Clostridium butyricum in the intestinal tract; Clostridium butyricum secretes vitamins and growth factors to promote the proliferation of probiotics, while secreting organic acids and antibacterial peptides to inhibit the growth of harmful bacteria; high fulvic acid sodium humate through the repair Intestinal mucosal damage can increase the level of probiotic adhesin receptors on the surface of intestinal mucosa, thereby promoting the colonization of probiotics in the intestinal tract. Through process coupling and functional synergy, the three can finally achieve the intestinal microecological balance of livestock and poultry and improve the intestinal tract. Barrier and absorption function, reduce diarrhea rate and improve feed utilization, thereby reducing the dependence on antibiotics in the breeding process.

Description

technical field [0001] The invention relates to an additive product and its application for synergistically promoting the proliferation and colonization of intestinal probiotics, in particular to an additive product that uses xylooligosaccharides, high fulvic acid sodium humate and Clostridium butyricum as raw materials, and utilizes the synergy of the three Coupling mechanism, additive products to promote the proliferation and colonization of intestinal probiotics and their application in livestock and poultry breeding. Background technique [0002] With the development of intensive farming, the application of antibiotics to prevent intestinal diseases of livestock and poultry is a relatively common method, but the indiscriminate use and abuse of antibiotics has led to the emergence of drug resistance and poses a safety threat to human health. The development of new additives suitable for the intestinal tract and micro-ecological environment of livestock and poultry will gr...

Claims

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Application Information

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IPC IPC(8): A23K20/163A23K20/10A23K10/18
Inventor 张杰程少博朱小玲丛晓燕王黎文肖林朱荣生丁健呼红梅位宾
Owner QILU UNIV OF TECH
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