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Culture medium of meningitis Neisseria

A technology of Neisseria and culture medium, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of unsuitable for large-scale enrichment, difficult source of materials, complicated production process, etc., and achieve a beneficial effect on colonies The effects of expansion, process simplification, and wide range of sources

Active Publication Date: 2011-04-06
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chocolate double-antibody agar is widely used as the separation medium at home and abroad. The separation and test effect of this medium is very good, but because fresh defibrinated sheep blood is used in the production, it has the disadvantages of difficult material source, high cost, and complicated production process. , not suitable for large-scale enrichment
In addition, most of the current meningitis culture medium is a separation medium, and its main purpose is to separate and detect, so in order to separate and detect, the existing culture medium should use antibiotics

Method used

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  • Culture medium of meningitis Neisseria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Prepare according to the following formula:

[0020] Sodium chloride 5.8g Potassium chloride 0.09g

[0021] Disodium hydrogen phosphate 2.6g L-glutamic acid 15.0g

[0022] L-Arginine 0.03g L-Tryptophan 0.0025g

[0023] L-cysteine ​​0.012g Calcium chloride 0.021g

[0024] Ferrous sulfate 0.002g Magnesium sulfate 0.6g

[0025] Soy peptone 15.0g Glucose 10.0g

[0026] Prepared by the following method:

[0027] A. Dissolve the mixture of sodium chloride, potassium chloride, disodium hydrogen phosphate, L-glutamic acid, L-arginine, L-cysteine, calcium chloride, ferrous sulfate and soybean peptone in distilled water , adjust the volume to 1L, adjust the pH to 7.4, and sterilize at 113°C and 0.08MPa for 15min;

[0028] B. Dissolve the mixture of L-tryptophan, magnesium sulfate and glucose in 15 mL of distilled water, filter and sterilize with a 0.2 μm disposable filter, and then add it to the mixture in step A that has been autoclaved to obtain a liquid medium.

Embodiment 2

[0030] Prepare according to the following formula:

[0031] Sodium chloride 3.0g Potassium chloride 0.05g

[0032] Disodium hydrogen phosphate 6.0g L-glutamic acid 5.0g

[0033] L-Arginine 0.01g L-Tryptophan 0.001g

[0034] L-cysteine ​​0.005g Calcium chloride 0.01g

[0035] Ferrous sulfate 0.008g Magnesium sulfate 1.0g

[0036] Soy peptone 5.0g Glucose 5.0g

[0037] Prepared by the following method:

[0038] A. Dissolve the mixture of sodium chloride, potassium chloride, disodium hydrogen phosphate, L-glutamic acid, L-arginine, L-cysteine, calcium chloride, ferrous sulfate and soybean peptone in distilled water , adjust the volume to 1L, adjust the pH to 7.2, and sterilize at 117°C and 0.06MPa for 15 minutes;

[0039] B. Dissolve the mixture of L-tryptophan, magnesium sulfate and glucose in 15 mL of distilled water, filter and sterilize with a 0.2 μm disposable filter, and then add it to the mixture in step A that has been autoclaved to obtain a liquid medium.

Embodiment 3

[0041] Prepare according to the following formula:

[0042] Sodium chloride 8.0g Potassium chloride 0.2g

[0043] Disodium hydrogen phosphate 2.0g L-glutamic acid 20.0g

[0044] L-Arginine 0.1g L-Tryptophan 0.01g

[0045] L-cysteine ​​0.03g Calcium chloride 0.1g

[0046] Ferrous sulfate 0.001g Magnesium sulfate 0.1g

[0047] Soy peptone 20.0g Glucose 20.0g

[0048] Prepared by the following method:

[0049] A. Dissolve the mixture of sodium chloride, potassium chloride, disodium hydrogen phosphate, L-glutamic acid, L-arginine, L-cysteine, calcium chloride, ferrous sulfate and soybean peptone in distilled water , adjust the volume to 1L, adjust the pH to 7.4, and sterilize at 115°C and 0.07MPa for 20min;

[0050] B. Dissolve the mixture of L-tryptophan, magnesium sulfate and glucose in 15 mL of distilled water, filter and sterilize with a 0.2 μm disposable filter, and then add it to the mixture in step A that has been autoclaved to obtain a liquid medium.

[0051] Neisse...

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Abstract

The invention provides a culture medium of meningitis Neisseria, which is characterized in that the culture medium consists of the following components: 3.0-8.0g / L of sodium chloride, 0.05-0.2g / L of potassium chloride, 1.0-6.0g / L of disodium hydrogen phosphate, 5-20g / L of L-glutamic acid, 0.01-0.1g / L of L-arginine, 0.001-0.01g / L of L-tryptophan, 0.005-0.030g / L of L-aminothiopropionic acid, 0.01-0.1g / L of calcium chloride, 0.001-0.008g / L of ferrous sulphate, 0.1-1 / 0 g / L of magnesium sulfate, 5.0-20.0 g / L of soya peptone and 5.0-20.0g / L of glucose. The invention is good for the growth and the metabolism of bacterium, can obtain higher output, has a wide material source, is free of structural constituents of the other animals, can effectively remove exogenous interference, simplifies technology on manufacture, is easy to operate, can effectively amplify the meningitis neisseria, and satisfies the mass production of vaccines.

Description

technical field [0001] The invention relates to a medium, in particular to an improved medium suitable for large-scale cultivation of Neisseria meningitidis, and belongs to the field of biotechnology. technical background [0002] Meningococcal meningitis, referred to as meningitis, is an acute respiratory infectious disease caused by Neisseria meningitidis. Due to the sudden onset, rapid development of the disease, and high mortality rate, the case fatality rate of the susceptible population—child fulminant type can reach 40% to 60%, so it has been widely paid attention to both at home and abroad. [0003] Although the research on Neisseria meningitidis was very early and achieved certain results, there are few relevant reports on its culture, and each manufacturer keeps its culture medium secret. Kan Fangqi et al. (CN 101089190A) invented a nutrient blood culture medium, which mainly provides general specimens and patient specimens that have used antibiotics and sulfonami...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/36
Inventor 李映波戴宗祥黄秋香毕湖冰张丽旌姬秋彦刘雅灵李剑锋徐维明
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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