Biological reinforcing method of hydrogenogen compensating material cultivation and biological hydrogen production system
A technology of biological hydrogen production and feeding culture, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of sludge loss, long reactor start-up time, low hydrogen production efficiency, etc., to achieve production Effects of improved hydrogen efficiency, shortened ethanol-type start-up time, and stable community structure
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specific Embodiment approach 1
[0007] Specific implementation mode 1: In this implementation mode, the hydrogen-producing bacteria feeding culture and the bioaugmentation method of the biological hydrogen production system are realized according to the following steps: 1. The cell concentration is 1×10 10 The hydrogen-producing bacteria liquid per L is inoculated in the culture solution of the fermenter at a volume ratio of 5% to 10%. Anaerobic fermentation is carried out in 99.99% nitrogen atmosphere; 2. After 20-30 hours of anaerobic fermentation, the fermentation liquid accounting for 50%-70% of the total fermentation volume is fed into the biological hydrogen production reaction equipment through a metering pump, and then fed to the fermentation tank The same volume of fresh culture solution was added to the medium, and the anaerobic fermentation was continued to complete the fed-batch culture of hydrogen-producing bacteria and the bioaugmentation of the biological hydrogen production system; the hydroge...
specific Embodiment approach 2
[0011] Specific embodiment two: the difference between this embodiment and specific embodiment one is that in the step one, inoculate in the culture medium of the fermentor with a volume ratio of 6% to 9%. 125~135r / min. Other steps and parameters are the same as those in Embodiment 1.
specific Embodiment approach 3
[0012] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 1, 8% by volume is inoculated into the culture medium of the fermenter, and the temperature of the fermenter is 36° C., and the stirring rate is 130 r / min. Other steps and parameters are the same as those in Embodiment 1.
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