Biological reinforcing method of hydrogenogen compensating material cultivation and biological hydrogen production system

A technology of biological hydrogen production and feeding culture, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of sludge loss, long reactor start-up time, low hydrogen production efficiency, etc., to achieve production Effects of improved hydrogen efficiency, shortened ethanol-type start-up time, and stable community structure

Active Publication Date: 2009-04-08
HARBIN INST OF TECH
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of long reactor start-up time, low hydrogen production efficiency, and sludge loss in the continuous operation process of the

Method used

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Experimental program
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specific Embodiment approach 1

[0007] Specific implementation mode 1: In this implementation mode, the hydrogen-producing bacteria feeding culture and the bioaugmentation method of the biological hydrogen production system are realized according to the following steps: 1. The cell concentration is 1×10 10 The hydrogen-producing bacteria liquid per L is inoculated in the culture solution of the fermenter at a volume ratio of 5% to 10%. Anaerobic fermentation is carried out in 99.99% nitrogen atmosphere; 2. After 20-30 hours of anaerobic fermentation, the fermentation liquid accounting for 50%-70% of the total fermentation volume is fed into the biological hydrogen production reaction equipment through a metering pump, and then fed to the fermentation tank The same volume of fresh culture solution was added to the medium, and the anaerobic fermentation was continued to complete the fed-batch culture of hydrogen-producing bacteria and the bioaugmentation of the biological hydrogen production system; the hydroge...

specific Embodiment approach 2

[0011] Specific embodiment two: the difference between this embodiment and specific embodiment one is that in the step one, inoculate in the culture medium of the fermentor with a volume ratio of 6% to 9%. 125~135r / min. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0012] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 1, 8% by volume is inoculated into the culture medium of the fermenter, and the temperature of the fermenter is 36° C., and the stirring rate is 130 r / min. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a fed culture method of hydrogen-producing bacteria and a biological enhancing method of a biological hydrogen producing system, which relates to a method for enhancing bacterial culture and the biological hydrogen producing system. The method solves the problems that the existing fermentation method has the defects of long startup time of a reactor, low hydrogen productivity and sludge loss during continuous operations of the biological hydrogen production. The method comprises the steps: A. hydrogen-producing bacteria are inoculated in nutrient solution in a fermentation tank for anaerobic fermentation; B. after the anaerobic fermentation, a part of fermentation solution is put into a biological hydrogen producing reacting device by a metering pump, and then fresh nutrient solution, which has the same volume as a part of fermentation solution put into the biological hydrogen producing reacting device, is added into the fermentation tank for ongoing anaerobic fermentation, thereby completing the fed culture of hydrogen-producing bacteria and the biological enhancement of the biological hydrogen producing system. The method shortens the startup time of the biological hydrogen producing reactor during the continuous operation to 15 days to 20 days, and increases the hydrogen productivity by 10 percent to 15 percent, thus avoiding the loss of active sludge.

Description

technical field [0001] The invention relates to a method for strengthening bacteria culture and hydrogen production system. Background technique [0002] The demand for energy is increasing day by day. However, the utilization and exploitation of fossil energy such as coal, oil, and natural gas have caused an energy crisis on the one hand, and new environmental problems on the other hand. The development of clean new energy technologies has become the focus of human attention. Hydrogen has entered the world's energy system due to its clean, efficient, renewable, and convenient storage and transportation, and has become a new energy source that has attracted much attention. Among various hydrogen production methods, the use of microorganisms to ferment organic wastewater to produce hydrogen has the characteristics of combining pollution control and production capacity, and has become a technology developed by various countries. Among them, fermentation biohydrogen production...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P3/00C12R1/01
Inventor 任南琪谢天卉邢德峰
Owner HARBIN INST OF TECH
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