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32results about How to "Overcoming incompatibility" patented technology

Method for sterile seeding and rapid propagation of bougainvillea speetabilis

The invention discloses a method for sterile seeding and rapid propagation of bougainvillea speetabilis. The method comprises the steps of conducting sterile seeding and induction germination on seeds, wherein the seeds are sterilized, then a shoot induction medium is inoculated with the sterilized seeds, and shoot induction culture is conducted at proper temperature with proper illumination time and illumination intensity; conducting seedling breeding through bud expanding propagation and rooting reinduction, wherein a sprout enrichment culture medium is inoculated with germinated buds, and bud propagation subculture is conducted at proper temperature with proper illumination time and illumination intensity; inoculating a rooting medium with the bud propagation subcultured bud seedlings, and conducting rooting breeding of seedlings at proper temperature with proper illumination time and illumination intensity. According to the method, seeds are used as breeding explant, seed treatment is easy, pollution rate is low, the germination rate of seeds reaches 100%, bud propagation rate reaches 5.22 times within 30 days, rooting rate is 95.6%, and seedling breeding is not limited by time or season so that whole-year production can be conducted.
Owner:海南省农业科学院热带园艺研究所

Method for obtaining, breeding and storing peanut interspecies hybridization variety, and identifying molecular cytology

The invention relates to a method for obtaining, breeding and storing a peanut interspecies hybridization variety F1, and identifying molecular cytology. The method comprises the following steps of: taking cultispecies white sand 1016 as a female parent and taking a wild seed A.macedoi as a male parent to carry out artificial hybridization; carrying out isolated culture on an ovule and a young embryo to obtain the F1; in a breeding process, placing a seedling terminal bud region into a solid rooting culture medium and culturing to obtain a whole set of a plant; in an identifying process, respectively extracting gene groups DNA (Deoxyribonucleic Acid) on the female parent, the male parent and the hybrid F1; utilizing specific markers E66 of the female parent and the male parent to carry out PCR (Polymerase Chain Reaction) reaction; and carrying out gel electrophoresis analysis to identify the reality of the hybrid F1. According to the method disclosed by the invention, great breakthroughs on the aspects of developing and utilizing a peanut wild species gene resource are obtained and the incompatibility of the interspecies hybridization is overcome, so that the hybrid F1 is bred and stored for a long period and a convenience condition is provided for transferring and utilizing good genes of the peanut wild species; the specific makers are combined with a molecular cytology technology to successfully identify the hybrid F1; and the method has the important active effects on a technology of enriching a heredity basis of peanut varieties and optimizing breeding.
Owner:HENAN ACAD OF AGRI SCI

Wheat hybridization method for increasing setting rate

The invention relates to the technical field of hybrid breeding, in particular to a wheat hybridization method for increasing the setting rate. The method comprises the steps that when wheat grows to a flower bud differentiation period, a wheat growth promoter is sprayed to male parents and female parents; after female parent ears are completely headed, flag leaves are stripped off, one third of each spikelet is cut off, stamens are removed, a pistil growth promoter is sprayed, glassine bags are taken, the female parent ears are covered with the glassine bags, test tubes containing water are fixed in the glassine bags, male parent ears are cut out to be placed in the test tubes which are fixed in the glassine bags and contain water when the selected male parents hybridized with the female parents grow to a flowering period, the glassine bags are shaken in a hybridizing pollination period to complete natural pollination between the male parents and the female parents, and after pollination is completed, the glassine bags and the test tubes are taken down; when the pollinated female parents grow to a filling period, a filling period regulator is sprayed. According to the method, the pollination method is combined with nutrient supply of wheat in different periods, false hybridization is avoided, the hybrid breeding efficiency is improved, and the setting rate and the yield are increased.
Owner:漯河市农业科学院

Fast breeding method of butterfly orchid and high-quality variety tissue culture propagation method

The invention discloses a fast breeding method of a butterfly orchid. Crossbreeding is carried out by utilizing an embryo culture technology, particularly, early-stage embryo culture technology is utilized to overcome the incompatibility of distant hybridization, and by rigidly controlling various links, including environmental conditions and breeding technology, of the whole breeding process, a cultured new variety of plants grow robustly and have excellent characters and a shortened breeding period, so that the fast breeding of a new variety and the selection of an excellent variety are realized. The invention further discloses a high-quality variety tissue culture propagation method of the butterfly orchid, for the selected high-quality new variety, an advantage that a pedicel internodesegment can be used for maintaining the characters of a stock plant in the tissue culturing process is utilized, pedicels of an excellent new variety, of which the seedling has stable characters, theblooming characters are excellent and the plant grows healthily, serve as an explant to perform tissue culture, and particularly preferential culture media are adopted in different phases, so that the butterfly orchid, which is cultured in a conventional condition, is fast in seedling emergence, good in stability, excellent in character and high in ornamental value.
Owner:青州市亚泰农业科技有限公司 +2

Insulating quilt of sunlight green house

The invention relates to an insulating quilt of a sunlight green house. The insulating quilt is characterized in that chemical fiber polyester wadding (2) with the grammage of 250-600g is used as a main body of the insulating quilt, wherein the lower surface of the chemical fiber polyester wadding (2) is formed into a matrix together with lower chemical fiber cloth (1) by a quilting method, and the upper surface of the chemical fiber polyester wadding (2) and the lower surface of upper chemical fiber cloth (4) are mutually bonded together by hot melting resin by a hot melting method; and four edges of the chemical fiber polyester wadding (2) and the chemical fiber cloth are sewed to obtain the insulating quilt. A fluffy body mainly containing textile waste is used as the insulating body, thus the insulating quilt has light weight, convenient use and low cost, and the price of the insulating quilt is within 1.5 times that of a straw curtain. The insulating quilt has good insulating effect, because rain water is not easy to enter the insulating main body, the insulating quilt cannot be frozen in winter, thus the insulating effect is outstanding. The insulating quilt solves the problem of incompatibility of various fibers and plastics, finishes the manufactures of a splicing layer, a waterproof layer and a heat conduction resistant layer at a time and reduces the cost.
Owner:楼杰

Method for obtaining sesame distant hybrid progeny through adopting immature embryo saving

The invention belongs to the field of biotechnical breeding, and concretely relates to a method for obtaining sesame distant hybrid progeny through adopting immature embryo saving. The method concretely comprises the following steps: selecting a sesame cultivar (2n = 26) as a female parent and a sesame wild species S. prostratum (2n = 58) as a male parent, or selecting a sesame wild species S. schinzianum (2n = 64) as a female parent and a semi-wild species S. malabaricum (2n = 26) as a male parent; carrying out distant hybirdization on the male parent and the female parent, taking immature capsules 10-18d after hybridization pollination, and picking off ovules; inoculating the ovules into an immature embryo induction medium, and carrying out culture induction on germs to make the germs germinate in order to obtain immature embryos; inoculating the immature embryos into a differentiation medium, and culturing until the immature embryos develop into hybrid F1 seedlings; and carrying out propagation spreading cultivation on the hybrid F1 seedlings to obtain complete hybrid F1 plants. The method adopts an immature embryo saving technology to overcome the problem of easy immature embryo and no obtaining of the hybrid F1 plants in the distant hybirdization process of the sesame wild species and the sesame cultivar, and allows the disease and adverse stress resistance of the wild species to be transferred to the cultivar in order to effectively improve the germplasm of the sesame cultivar.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI

Method for inducing bougainvillea speetabilis polyploidy in vitro

PendingCN114667927AOvercoming incompatibilityOvercome the phenomenon of low hybrid seed setting ratePlant tissue cultureHorticulture methodsPolyploidHypocotyl
The invention provides a method for in-vitro induction of bougainvillea speetabilis polyploidy, which comprises the following steps: hybridizing a diploid bougainvillea speetabilis var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra and inoculating the pre-cultured hypocotyl into a differential culture medium, performing polyploidy induction culture for 30 days, and sequentially performing multiplication culture and rooting culture on survived differentiated buds in a multiplication culture medium and a rooting culture medium. Colchicine containing dimethyl sulfoxide is used for carrying out doubling induction on a bougainvillea speetabilis sterile material, so that bougainvillea speetabilis chromosomes are doubled, a polyploidy plant is obtained, and breeding ways of bougainvillea speetabilis are increased. The plant polyploid not only has the advantages of strong growth vigor, rapid growth, prolonged ornamental period, strong comprehensive resistance and the like, but also can improve the plant fertility, and can provide new germplasm for large-scale bougainvillea speetabilis crossbreeding.
Owner:TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI

Table top-fixing method and device and computer readable storage medium

The invention relates to a table top-fixing method. The table top-fixing method comprises the following steps: adding a first Stack component and a second Stack component to a view; adding a Container component to a first area of the upper layer of the first Stack component, and adding a transverse list component to a second area of the lower layer of the first Stack component, wherein the first area is an initial display area of the first cell of the table in the view, the table is pre-configured as first-row fixed logic, the second area is an initial display area of the first row of the table in the view; and adding a longitudinal list component to a third area of the upper layer of the second Stack component, and adding a StickyHeadersTable component to a fourth area of the lower layer of the second Stack component, wherein the transverse list component is used for synchronizing the monitored transverse rolling position to the second Stack component, and the StickyHeadersTable component is used for synchronizing the monitored transverse rolling position to the first Stack component. By using the scheme provided by the invention, the first-row top-fixing effect and the first-column top-fixing effect of the table can be realized. The invention further provides a table top-fixing device and a computer readable storage medium.
Owner:杭州时戳信息科技有限公司

Method for increasing mango high-branch grafting survival rate

The invention discloses a method for increasing mango high-branch grafting survival rate. The method includes the steps that a regulator I is sprayed to a scion tree body of a scion to be collected before collecting the scion; an annular ditch is dug around a rootstock tree body to be subjected to high-branch grafting before grafting, and a regulator II is applied in a burying mode; tree crowns are trimmed before grafting; during high branch grafting, after stock and scion incisions are cut, beta-1,3-1, and 4 glucanase solutions are dipped and smeared on the stock incisions and the scion incisions respectively, and then the scion incisions are connected with the stock incisions for grafting; and a regulator III is sprayed to the whole plant after grafting is completed. By the adoption of the method for increasing the mango high-branch grafting survival rate, the survival rate of mango high branch grafting is increased from 30-40% to 65-85% under the condition that the affinity degree between a stock variety and a scion variety is low, the basic healing speed of a grafting opening is increased by 10-15 days or above compared with a conventional method, new scion shoots do not need to be unbound after being drawn out, the surface of the grafting opening is smoother, and the probability of suffering from microbial diseases in a later period is reduced.
Owner:YUNNAN INST OF TROPICAL CROPS

A method for obtaining distant hybrids of sesame wild species and cultivated species by using immature embryo rescue technology

InactiveCN105104186BGood disease and stress resistanceImproved germplasmPlant tissue cultureHorticulture methodsDiseaseWild species
The invention belongs to the field of biotechnical breeding, and concretely relates to a method for obtaining sesame wild species and cultivar distant hybrid progeny through adopting an immature embryo saving technology. The method concretely comprises the following steps: selecting a sesame cultivar (2n = 26) as a female parent and sesame wild species (2n=64) as a male parent; carrying out distant hybirdization on the male parent and the female parent, taking immature capsules 10-18d after hybridization pollination, and picking off ovules; inoculating the ovules into an immature embryo induction medium, and carrying out culture induction on germs to make the germs germinate in order to obtain immature embryos; inoculating the immature embryos into a differentiation medium, and culturing until the immature embryos develop into hybrid F1 seedlings; and carrying out propagation spreading cultivation on the hybrid F1 seedlings to obtain complete hybrid F1 plants. The method adopts the immature embryo saving technology to overcome the problem of easy immature embryo and no obtaining of the hybrid F1 plants in the distant hybirdization process of the sesame wild species and the sesame cultivar, and allows the disease and adverse stress resistance of the wild species to be transferred to the cultivar in order to effectively improve the germplasm of the sesame cultivar.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI

A Wheat Hybrid Method for Improving Seed Setting Rate

The invention relates to the technical field of hybrid breeding, in particular to a wheat hybridization method for increasing the setting rate. The method comprises the steps that when wheat grows to a flower bud differentiation period, a wheat growth promoter is sprayed to male parents and female parents; after female parent ears are completely headed, flag leaves are stripped off, one third of each spikelet is cut off, stamens are removed, a pistil growth promoter is sprayed, glassine bags are taken, the female parent ears are covered with the glassine bags, test tubes containing water are fixed in the glassine bags, male parent ears are cut out to be placed in the test tubes which are fixed in the glassine bags and contain water when the selected male parents hybridized with the female parents grow to a flowering period, the glassine bags are shaken in a hybridizing pollination period to complete natural pollination between the male parents and the female parents, and after pollination is completed, the glassine bags and the test tubes are taken down; when the pollinated female parents grow to a filling period, a filling period regulator is sprayed. According to the method, the pollination method is combined with nutrient supply of wheat in different periods, false hybridization is avoided, the hybrid breeding efficiency is improved, and the setting rate and the yield are increased.
Owner:漯河市农业科学院

Method for obtaining, breeding and storing peanut interspecies hybridization variety, and identifying molecular cytology

ActiveCN102960237BOvercoming Distant Hybrid SterilityOvercoming infertilityMicrobiological testing/measurementHorticulture methodsWild speciesAnimal science
The invention relates to a method for obtaining, breeding and storing a peanut interspecies hybridization variety F1, and identifying molecular cytology. The method comprises the following steps of: taking cultispecies white sand 1016 as a female parent and taking a wild seed A.macedoi as a male parent to carry out artificial hybridization; carrying out isolated culture on an ovule and a young embryo to obtain the F1; in a breeding process, placing a seedling terminal bud region into a solid rooting culture medium and culturing to obtain a whole set of a plant; in an identifying process, respectively extracting gene groups DNA (Deoxyribonucleic Acid) on the female parent, the male parent and the hybrid F1; utilizing specific markers E66 of the female parent and the male parent to carry out PCR (Polymerase Chain Reaction) reaction; and carrying out gel electrophoresis analysis to identify the reality of the hybrid F1. According to the method disclosed by the invention, great breakthroughs on the aspects of developing and utilizing a peanut wild species gene resource are obtained and the incompatibility of the interspecies hybridization is overcome, so that the hybrid F1 is bred and stored for a long period and a convenience condition is provided for transferring and utilizing good genes of the peanut wild species; the specific makers are combined with a molecular cytology technology to successfully identify the hybrid F1; and the method has the important active effects on a technology of enriching a heredity basis of peanut varieties and optimizing breeding.
Owner:HENAN ACAD OF AGRI SCI

Method for obtaining interspecific filial generation of dianthus superbus and dianthus caryophyllus through embryo rescue

The invention belongs to the field of biotechnology breeding, and particularly relates to a method for obtaining interspecific filial generation of dianthus superbus and dianthus caryophyllus through embryo rescue, and the method specifically comprises the following steps: selecting dianthus superbus L as a female parent, selecting dianthus caryophyllus L as a male parent, selecting dianthus caryophyllus L as a male parent, selecting dianthus caryophyllus L as a female parent, selecting dianthus caryophyllus L as a male parent, selecting dianthus caryophyllus L as a female parent, and selecting dianthus caryophyllus L as a male parent; performing artificial pollination for interspecific hybridization; carrying out in-vitro culture on ovules; and culturing young embryos and transplanting seedlings. According to the method, the obstacle that hybrid plants cannot be obtained due to abortion of immature embryos during interspecific hybridization of the dianthus superbus and the dianthus caryophyllus is overcome by utilizing an embryo rescue technology, a large number of filial generations are obtained, and the hybrid plants inherit excellent characters of parents, are low and robust, are high in stress resistance, are large in flowers, bright in color and rich in flower fragrance and have extremely high ornamental value. According to the method, the interspecific cross breeding efficiency of the dianthus plants can be effectively improved, and the new variety breeding process of the dianthus flowers is accelerated.
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI

Insulating quilt of sunlight green house

The invention relates to an insulating quilt of a sunlight green house. The insulating quilt is characterized in that chemical fiber polyester wadding (2) with the grammage of 250-600g is used as a main body of the insulating quilt, wherein the lower surface of the chemical fiber polyester wadding (2) is formed into a matrix together with lower chemical fiber cloth (1) by a quilting method, and the upper surface of the chemical fiber polyester wadding (2) and the lower surface of upper chemical fiber cloth (4) are mutually bonded together by hot melting resin by a hot melting method; and fouredges of the chemical fiber polyester wadding (2) and the chemical fiber cloth are sewed to obtain the insulating quilt. A fluffy body mainly containing textile waste is used as the insulating body, thus the insulating quilt has light weight, convenient use and low cost, and the price of the insulating quilt is within 1.5 times that of a straw curtain. The insulating quilt has good insulating effect, because rain water is not easy to enter the insulating main body, the insulating quilt cannot be frozen in winter, thus the insulating effect is outstanding. The insulating quilt solves the problem of incompatibility of various fibers and plastics, finishes the manufactures of a splicing layer, a waterproof layer and a heat conduction resistant layer at a time and reduces the cost.
Owner:楼杰

A method of rescuing immature embryos to obtain the offspring of sesame distant hybrids

The invention belongs to the field of biotechnical breeding, and concretely relates to a method for obtaining sesame distant hybrid progeny through adopting immature embryo saving. The method concretely comprises the following steps: selecting a sesame cultivar (2n = 26) as a female parent and a sesame wild species S. prostratum (2n = 58) as a male parent, or selecting a sesame wild species S. schinzianum (2n = 64) as a female parent and a semi-wild species S. malabaricum (2n = 26) as a male parent; carrying out distant hybirdization on the male parent and the female parent, taking immature capsules 10-18d after hybridization pollination, and picking off ovules; inoculating the ovules into an immature embryo induction medium, and carrying out culture induction on germs to make the germs germinate in order to obtain immature embryos; inoculating the immature embryos into a differentiation medium, and culturing until the immature embryos develop into hybrid F1 seedlings; and carrying out propagation spreading cultivation on the hybrid F1 seedlings to obtain complete hybrid F1 plants. The method adopts an immature embryo saving technology to overcome the problem of easy immature embryo and no obtaining of the hybrid F1 plants in the distant hybirdization process of the sesame wild species and the sesame cultivar, and allows the disease and adverse stress resistance of the wild species to be transferred to the cultivar in order to effectively improve the germplasm of the sesame cultivar.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI

Identification and isolation method of through faults of coal mine power supply system

InactiveCN103094987BReduce the scope of outagesSolve the problem of non-selectivityCircuit arrangementsSmart substationDisplay device
The invention provides an identification and isolation method of through faults of a coal mine power supply system, and belongs to a relay protection method of the coal mine power supply system. The steps of the method include that (1) sub intelligent substations are built in substations on the ground and underground, a station level with an area centralized control center is built on the ground to serve as a common station level of all the sub intelligent substations, and the common station level coordinates and controls the sub intelligent substations; (2) a host in the centralized control center collects and comprehensively judges fault information sent from protection and measurement and control modules of the sub intelligent substations to achieve identification and isolation of the faults, displays fault sections and fault types on a displayer and sends out fault alarms; and (3) the sub intelligent substations collect or samples system voltage and current data of each branch circuit, and calculate and judge the various fault types and abnormal operation states, and the sub intelligent substations send out trip orders to breakers at the fault sections to achieve isolation of the various types of faults. The method has the advantages of being easy to carry out, precise in judge and high in reliability.
Owner:CHINA UNIV OF MINING & TECH
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