32results about How to "Overcoming incompatibility" patented technology

The invention belongs to the field of biotechnical breeding, and concretely relates to a method for obtaining sesame wild species and cultivar distant hybrid progeny through adopting an immature embryo saving technology. The method concretely comprises the following steps: selecting a sesame cultivar (2n = 26) as a female parent and sesame wild species (2n=64) as a male parent; carrying out distant hybirdization on the male parent and the female parent, taking immature capsules 10-18d after hybridization pollination, and picking off ovules; inoculating the ovules into an immature embryo induction medium, and carrying out culture induction on germs to make the germs germinate in order to obtain immature embryos; inoculating the immature embryos into a differentiation medium, and culturing until the immature embryos develop into hybrid F1 seedlings; and carrying out propagation spreading cultivation on the hybrid F1 seedlings to obtain complete hybrid F1 plants. The method adopts the immature embryo saving technology to overcome the problem of easy immature embryo and no obtaining of the hybrid F1 plants in the distant hybirdization process of the sesame wild species and the sesame cultivar, and allows the disease and adverse stress resistance of the wild species to be transferred to the cultivar in order to effectively improve the germplasm of the sesame cultivar.

The invention relates to the technical field of hybrid breeding, in particular to a wheat hybridization method for increasing the setting rate. The method comprises the steps that when wheat grows to a flower bud differentiation period, a wheat growth promoter is sprayed to male parents and female parents; after female parent ears are completely headed, flag leaves are stripped off, one third of each spikelet is cut off, stamens are removed, a pistil growth promoter is sprayed, glassine bags are taken, the female parent ears are covered with the glassine bags, test tubes containing water are fixed in the glassine bags, male parent ears are cut out to be placed in the test tubes which are fixed in the glassine bags and contain water when the selected male parents hybridized with the female parents grow to a flowering period, the glassine bags are shaken in a hybridizing pollination period to complete natural pollination between the male parents and the female parents, and after pollination is completed, the glassine bags and the test tubes are taken down; when the pollinated female parents grow to a filling period, a filling period regulator is sprayed. According to the method, the pollination method is combined with nutrient supply of wheat in different periods, false hybridization is avoided, the hybrid breeding efficiency is improved, and the setting rate and the yield are increased.

The invention discloses a method for quickly identifying differential protein during interaction of chrysanthemum pollen and stigma, belonging to the field of chrysanthemum proteomics. The method comprises the following steps of: performing distant hybridization work of chrysanthemum by taking cultured chrysanthemum as a female parent and wild chrysanthemum as a male parent; cutting off pollinated inflorescences in 1 h and 24 h after pollination; removing petals and sepals of ligulate flowers in the obtained inflorescences to obtain pistils; extracting a protein sample from the obtained pistils by using a TCA (Trichloroacetic Acid) / acetone precipitation method; and quickly identifying the differential protein by applying iTRAQ (Isobaric Tags For Relative And Absolute Quantitation) and a mass spectrum technology and performing bioinformatic analysis. Aiming at the chrysanthemum and wild species of related genera thereof, the invention provides a method for quickly and accurately identifying key differential protein during the interaction of the chrysanthemum pollen and the stigma for providing research foundation for solving the problem of distant hybridization incompatibility of the chrysanthemum.

The invention belongs to the technical field of high polymer materials and relates to a resin material and a preparation method thereof. The resin material comprises the following components in parts by weight: 10 to 40 parts of crushing material of waste polyester packages, 35 to 80 parts of high polymer resin, 3 to 10 parts of compatilizer, 3 to 15 parts of toughening agent, 0.5 to 1 part of antioxidant aid and 0.2 to 0.5 part of processing aid. The resin material is prepared by using a double-screw extrusion process, the condition that a large amount of polyester is hydrolyzed is avoided in a production process, and the problem that the two main base materials are incompatible is solved. According to the resin material, the performance reaches the performance of the primary novel material which is produced in the petrochemical industry, recovered polyester packages can be converted into a high-quality plastic material, and compared with the method for degrading and drawing the recovered polyester package into short fibers or woven bags, the resin material has good economic benefits.

The invention relates to a method for obtaining, breeding and storing a peanut interspecies hybridization variety F1, and identifying molecular cytology. The method comprises the following steps of: taking cultispecies white sand 1016 as a female parent and taking a wild seed A.macedoi as a male parent to carry out artificial hybridization; carrying out isolated culture on an ovule and a young embryo to obtain the F1; in a breeding process, placing a seedling terminal bud region into a solid rooting culture medium and culturing to obtain a whole set of a plant; in an identifying process, respectively extracting gene groups DNA (Deoxyribonucleic Acid) on the female parent, the male parent and the hybrid F1; utilizing specific markers E66 of the female parent and the male parent to carry out PCR (Polymerase Chain Reaction) reaction; and carrying out gel electrophoresis analysis to identify the reality of the hybrid F1. According to the method disclosed by the invention, great breakthroughs on the aspects of developing and utilizing a peanut wild species gene resource are obtained and the incompatibility of the interspecies hybridization is overcome, so that the hybrid F1 is bred and stored for a long period and a convenience condition is provided for transferring and utilizing good genes of the peanut wild species; the specific makers are combined with a molecular cytology technology to successfully identify the hybrid F1; and the method has the important active effects on a technology of enriching a heredity basis of peanut varieties and optimizing breeding.

The invention discloses a method for overcoming distant hybridization obstruction of s poplar, in particular a method for processing a female parent pistil stigma in hybridization parents, which comprises the following steps of: (1) fumigating the pistil stigma of the female parent by volatile gas of normal hexane; and (2) coating aqueous extract solution of pollen of the female parent on the pistil stigma of the female parent processed through the step (1), wherein the female parent is a populus poplar, and the aqueous extract solution of the pollen is liquid which is obtained by extracting the pollen of the female parent through water and does not contain pollen grains. Due to the utilization of the method, incompatibility of hybridization between poplar sections or intro-sections can be effectively overcome, and particularly, the hybridization obstruction of poplar of white poplar section and populus aigeiros is effectively overcome. The experimental results show that the hybridization seeds obtained through the method has high maturing rate and good plumpness, the germination percentage of the seeds is more than 70 percent, and a new way is provided for obtaining an excellent hybridal clone for distant hybridization of the poplar.

The invention relates to an insulating quilt of a sunlight green house. The insulating quilt is characterized in that chemical fiber polyester wadding (2) with the grammage of 250-600g is used as a main body of the insulating quilt, wherein the lower surface of the chemical fiber polyester wadding (2) is formed into a matrix together with lower chemical fiber cloth (1) by a quilting method, and the upper surface of the chemical fiber polyester wadding (2) and the lower surface of upper chemical fiber cloth (4) are mutually bonded together by hot melting resin by a hot melting method; and fouredges of the chemical fiber polyester wadding (2) and the chemical fiber cloth are sewed to obtain the insulating quilt. A fluffy body mainly containing textile waste is used as the insulating body, thus the insulating quilt has light weight, convenient use and low cost, and the price of the insulating quilt is within 1.5 times that of a straw curtain. The insulating quilt has good insulating effect, because rain water is not easy to enter the insulating main body, the insulating quilt cannot be frozen in winter, thus the insulating effect is outstanding. The insulating quilt solves the problem of incompatibility of various fibers and plastics, finishes the manufactures of a splicing layer, a waterproof layer and a heat conduction resistant layer at a time and reduces the cost.

The invention belongs to the field of biotechnical breeding, and concretely relates to a method for obtaining sesame distant hybrid progeny through adopting immature embryo saving. The method concretely comprises the following steps: selecting a sesame cultivar (2n = 26) as a female parent and a sesame wild species S. prostratum (2n = 58) as a male parent, or selecting a sesame wild species S. schinzianum (2n = 64) as a female parent and a semi-wild species S. malabaricum (2n = 26) as a male parent; carrying out distant hybirdization on the male parent and the female parent, taking immature capsules 10-18d after hybridization pollination, and picking off ovules; inoculating the ovules into an immature embryo induction medium, and carrying out culture induction on germs to make the germs germinate in order to obtain immature embryos; inoculating the immature embryos into a differentiation medium, and culturing until the immature embryos develop into hybrid F1 seedlings; and carrying out propagation spreading cultivation on the hybrid F1 seedlings to obtain complete hybrid F1 plants. The method adopts an immature embryo saving technology to overcome the problem of easy immature embryo and no obtaining of the hybrid F1 plants in the distant hybirdization process of the sesame wild species and the sesame cultivar, and allows the disease and adverse stress resistance of the wild species to be transferred to the cultivar in order to effectively improve the germplasm of the sesame cultivar.

The invention discloses a carrier-free foaming masterbatch and a preparation method thereof. The carrier-free foaming masterbatch adopts a low molecular weight coupling agent as a bonding component, and mixes a foaming agent, a foaming nucleating agent, a processing aid and Components such as fillers are prepared by extrusion granulation. The extrusion granulation temperature of the product of the present invention is lower than 80°C, the foaming agent will not decompose during the masterbatch preparation process, the foaming agent has a high degree of dispersion, and the subsequent foaming molding can produce finer and more uniform micropores Foamed products have good compatibility with engineering plastics. In addition, the present invention can prepare a high-concentration foaming masterbatch product with a foaming agent content of 80%, the process is simple and convenient, and the production cost is greatly reduced.

The invention provides an identification and isolation method of through faults of a coal mine power supply system, and belongs to a relay protection method of the coal mine power supply system. The steps of the method include that (1) sub intelligent substations are built in substations on the ground and underground, a station level with an area centralized control center is built on the ground to serve as a common station level of all the sub intelligent substations, and the common station level coordinates and controls the sub intelligent substations; (2) a host in the centralized control center collects and comprehensively judges fault information sent from protection and measurement and control modules of the sub intelligent substations to achieve identification and isolation of the faults, displays fault sections and fault types on a displayer and sends out fault alarms; and (3) the sub intelligent substations collect or samples system voltage and current data of each branch circuit, and calculate and judge the various fault types and abnormal operation states, and the sub intelligent substations send out trip orders to breakers at the fault sections to achieve isolation of the various types of faults. The method has the advantages of being easy to carry out, precise in judge and high in reliability.