Method for obtaining yellow-seed cabbage type rape

A technology of Brassica napus and its acquisition method, which is applied in the field of obtaining new germplasm resources of yellow seeds of Brassica napus, can solve problems such as genetic instability and impure seed color, achieve the expansion of genetic variation, realize inter-genus transfer, overcome Effects of outcross incompatibility

Inactive Publication Date: 2017-05-10
重庆泰升生态农业发展有限公司
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Problems solved by technology

The yellow-seeded Brassica napus bred so far is all derived from interspecific hybridization within the genus Brassica, and there have been no reports of obtaining yellow-seeded Brassica napus through inter-genus hybridization, and the yellow-seeded traits of Brassica napus derived from within the genus are currently obtained There are problems of impure seed color and genetic instability

Method used

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  • Method for obtaining yellow-seed cabbage type rape
  • Method for obtaining yellow-seed cabbage type rape

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Embodiment Construction

[0016] 1. The steps for obtaining germplasm resources of yellow-seeded Brassica napus, such as figure 1 Shown:

[0017] 1. Protoplast fusion and plant regeneration of wild species of S. white mustard and Brassica napus:

[0018] Sterilize wild seeds of S. alba and Brassica oleracea by 70% (v / v) alcohol for 1 min, 2% (v / v) sodium hypochlorite for 15 min, rinse with sterile water three times, and inoculate In MS medium, cultured for 16 hours at 25°C under light conditions.

[0019] The leaves of 3-4 weeks old were taken respectively, and the protoplasts of S. sinensis and Brassica oleracea were isolated respectively.

[0020] Mix the protoplasts of S. sinensis and Brassica oleracea in a volume ratio of 1:2 to a final concentration of ≥5×10 5 cells / mL, electrofusion was performed.

[0021] The electrofused original plastids were cultured in the dark at 25°C in CR medium, and the callus formed after 2 to 3 weeks was transferred to solid medium (MS+10g / l sucrose+20g / l mannitol+...

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Abstract

The invention relates to a method for obtaining yellow-seed cabbage type rape, belonging to the technical field of agriculture. The method comprises the following steps: subjecting Sinapsis white mustard wild species and cabbage type rape to protoplast fusion so as to obtain intergeneric hybrid F1, subjecting parental cabbage type rape to backcrossing so as to obtain BC2, selecting tawny-seed single plants from self-crossed progenies of BC2, and subjecting the single plants to further self-crossing so as to obtain genetically-stable and different-level yellow-seed phenotype strains, wherein cytological and molecular biological identification results show that yellow-seed materials are translocation line materials with white mustard yellow-seed genes. According to the invention, a near infrared spectrometer TR-3700 of a Denmark FOSS company is used to determine the oil content of yellow-seed strains, and the determination results show that the oil content of yellow-seed single plants obtained by using the method provided by the invention is significantly higher than the oil content of the Sinapsis white mustard plants, and is increased by 1.7 to 6% compared with the oil content of Brassica cabbage type plants.

Description

technical field [0001] The invention belongs to the field of agricultural technology, in particular to the acquisition technology and identification method of new germplasm resources of Brassica napus yellow seeds. Background technique [0002] Yellow-seed rape has the advantages of relatively high oil content, good quality rapeseed oil, and high nutritional value of rapeseed meal. Breeding yellow-seed rape has become one of the main goals of current rapeseed breeding, and has become a way to increase seed oil content and improve rapeseed quality. important way. Both cabbage-type rape and mustard-type rape have many yellow seed varieties, and the yellow seed traits are stable. However, there is no natural yellow seed germplasm in Brassica napus, and yellow seed coat gene resources are scarce. The yellow-seeded Brassica napus bred so far is all derived from interspecific hybridization within the genus Brassica, and there have been no reports of obtaining yellow-seeded Brass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001
Inventor 黄文君夏天雨
Owner 重庆泰升生态农业发展有限公司
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