Method for quickly identifying differential protein during interaction of chrysanthemum pollen and stigma
A technology of differential protein and chrysanthemum pollen, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve problems such as low seed setting rate and inability to obtain hybrid seeds
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[0022] 1. Use the commercially available cultivated chrysanthemum 'Yuhua Xingchen' as the female parent, select well-developed flower buds, emasculate when the tongue-shaped flowers are just exposed, and remove all the bisexual tubular florets in the inner ring, and cut off the tongue-shaped flower petals at the same time Until the stigma is visible (the stigma cannot be hurt), and bagged strictly. The male parent is isolated by bagging before the flower heads open and loose powder. When the stigma of the female parent protrudes and splits to present acute angles and secretes mucus (3 to 5 days after emasculation), collect fresh pollen from the bagged male parent chrysanthemum brain, pollinate the female parent with a brush, and pollinate the female parent immediately after pollination. Ben continued to be bagged and quarantined. 1h and 24h after pollination, the pollinated inflorescences were cut respectively, immediately snap-frozen in liquid nitrogen and stored at -80°C fo...
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